Homogeneous luminescent immunoassay method for simultaneous quantitative analysis of multiple components and kit used therefor
A quantitative analysis and homogeneous luminescence technology, which is applied in the field of clinical examination and diagnosis, can solve the problems of high detection cost, complex and unsuitable biochip technology, and achieve the effects of simple operation, saving specimen consumption, and high precision
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[0026] Taking the simultaneous quantitative analysis of serum alpha-fetoprotein (AFP), carcinoembryonic antigen (CEA), sugar chain antigen 125 (CA-125), and ferritin (Ferr) as an example, the homogeneous luminescence immunoassay for simultaneous determination of multiple components is described in detail. Analytical methods (such as image 3 ): There are four receptor microspheres in the detection system, and the four receptor microspheres all contain the same chemiluminescence agent and different fluoresceins, among which 1# receptor microspheres contain FITC, and 2# receptor microspheres contain PE, 3# receptor microspheres contain EDC, 4# receptor microspheres contain PE-Cy5), each receptor microsphere is coated with a polyclonal antibody, 1# receptor microspheres are anti-AFP, 2 # acceptor microspheres are anti-CEA, 3# acceptor microspheres are anti-CA-125, and 4# acceptor microspheres are anti-Ferr. In addition, there are four biotinylated monoclonal antibodies in the sy...
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