Kit for detecting phosphorylated epidermal growth factor receptor and preparation method thereof

A technology of epidermal growth factor and kit, applied in the field of biomedicine, can solve the problems of cumbersome operation, low sensitivity, expensive instruments, etc., and achieve the effect of overcoming cumbersome operation and less specimen consumption
CN101858909BActive Publication Date: 2013-07-03RAYBIOTECH INC GUANGZHOU
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
RAYBIOTECH INC GUANGZHOU
Publication Date
2013-07-03

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Abstract

The invention discloses a kit for detecting phosphorylated epidermal growth factor receptor and the kit adopts the protein chip technology. The kit comprises a base film (1) fixed with several kinds of specific antibodies and reactant and detection agent (2), wherein specific antibodies and phosphorylated epidermal growth factor receptor can perform antibody-antigen reaction, each kind of specific antibodies are fixed on a plurality of independent recognition sites which are fixed on the base film; and the reactant and detection agent (2) are used to detect whether the substance which can perform antibody-antigen reaction with specific antibodies exists in a sample to be detected through the array-ELISA method. The invention also discloses a preparation method of the kit. By adopting the kit of the invention, 17 phosphorylated epidermal growth factor receptors in a plurality of samples can be detected, lots of defects of the prior art can be overcome and the kit has the advantages that the kit is cheap, convenient, sensitive and accurate, has high flux and less amount of sample and can be popularized in the ordinary laboratory and scaled, etc.
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Description

technical field

[0001] The invention relates to the technical field of biomedicine, in particular to a protein chip kit for detecting phosphorylated epidermal growth factor receptor and a preparation method thereof. technical background

[0002] Cancer, also known as malignant neoplasm in medical terms, is a disease caused by abnormalities in the mechanism of controlling cell growth and proliferation. In addition to growing uncontrollably, cancer cells also partially invade surrounding normal tissues (invasion) and even transfer to other parts of the body through the internal circulatory system or lymphatic system, which is called cancer cell invasion in the pathology community. Cancer is a multifactorial disease. Its etiology may be related to heredity, gene mutation, aging, or environmental factors. Due to the high morbidity and mortality, it seriously threatens the health of the public. Cancer has become one of the leading causes of death in developed countries. [00...

Claims

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