Antibody chip kit for simultaneous quantitative detection of multiple receptors

Abstract

The invention discloses an antibody chip kit for simultaneous quantitative detection of multiple receptors. The kit comprises antibody chips, a receptor standards mixture, a biotin labeled detection antibodies mixture and Cy3 fluorochrome labeled streptavidin. A standard tissue slide which is coated by reactive amino groups is chosen as a surface carrier of the antibody chips, and various antigen specific antibodies are adsorbed firmly on the surface of the slide by a non-contact spotting robot and sixteen microarrays are formed. The sixteen microarrays are then separated in non-interfering small areas by the use of a plastic frame with 2*8 holes and a latex closed frame which match each other and are demountable. Each capture antibody has four repeated spottings in each antibody microarray. The antibody chip kit can realize simultaneous quantitative detection of multiple receptors. Dynamic detection range of receptors is wider. Quadruple ELISA repeated data can be obtained from a single sample experiment. The antibody chip kit has advantages of high sensitivity, high throughput, small required use amount of samples, low cost, easy popularization and the like.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to an antibody chip kit for quantitatively detecting multiple receptors at the same time. technical background [0002] A receptor is a protein that can bind to a specific extracellular signaling molecule (ligand) to trigger a cellular response. [0003] Receptors are divided into cell surface receptors and intracellular receptors. When the receptor binds to the ligand, the molecular conformation changes, thereby causing cellular responses, such as mediating cellular processes such as intercellular signal transduction, intercellular adhesion, and cell endocytosis. Receptors can recognize bioactive molecules on the cell membrane or in the cell and bind to them, amplify and transmit the recognized and received signals to the inside of the cell, thereby activating or starting a series of biochemical reactions, and finally lead to the specificity of the signal substance. biologica...

AI Technical Summary

Problems solved by technology

However, one test can only detect a single indicator, with low throughput and high cost. In the detection of receptors with multi-indicator characteristics, this method has obvious shortcomings; although radioimmunoassay (RIA) has high sensitivity, it is due to radioactive contamination. It is rarely used now; immunoblotting can measure the size of molecules, and there is no non-specific reaction, but the operation is cumbersome, the sensitivity is low, and it can only detect a single indicator, which is not suitable for the detection of receptors; flow cytometry can be used in the detection of receptors. Detect the level of receptors at the cellular level, but there are disadvantages such as low sensitivity, low throughput, and high cost

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