Cell electrochemical sensor for analyzing joint toxicity of fungaltoxin

A cell sensor and mycotoxin technology, which is applied in the fields of electrochemical variables of materials, scientific instruments, analytical materials, etc., can solve the problems of inability to effectively simulate the actual state of cells, cumbersome electrode modification process, and inability to mass-produce at the same time. Low production cost and quick response

Active Publication Date: 2017-09-29
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although there have been some related reports on the use of physical adsorption or chemical interaction to fix cells on the electrode surface, most of these studies use traditional glassy carbon electrodes, which are costly (not less than 700 yuan per electrode), The electrode modification process is cumbersome and cannot be mass-produced at the same time
In addition, these reported methods cannot effectively simulate the actual state of cells growing in situ in the human body

Method used

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  • Cell electrochemical sensor for analyzing joint toxicity of fungaltoxin
  • Cell electrochemical sensor for analyzing joint toxicity of fungaltoxin
  • Cell electrochemical sensor for analyzing joint toxicity of fungaltoxin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Example 1 Preparation of cell electrochemical sensor

[0045] (1) Using cyclic voltammetry at 2.5mM Fe(CN) 6 3- / 4- Scan ten cycles from -0.2V to 0.6V in the electrolyte to remove impurities on the electrode surface, then clean the screen-printed carbon electrode with ultrapure water, and dry it with nitrogen gas for later use;

[0046] (2) Electroplating and depositing gold nanoparticles on the surface of the working screen printing electrode, electroplating method: the working electrode is placed in 1mmol L -1 Chlorauric acid and 0.5mol·L -1 In the sulfuric acid solution, under the potential of -0.25V, the electroplating time was 120s to complete the electroplating. After electroplating, it was cleaned with ultrapure water and dried with nitrogen to obtain a gold nano-modified screen-printed electrode;

[0047] (3) Dip the gold nano / screen-printed electrode into 18mmol L -1 The mercaptoethylamine solution was reacted at room temperature in the dark for 4 hours, so ...

Embodiment 2

[0051] Example 2 Application of cell electrochemical sensor

[0052] 1. Mycotoxin-stimulated cellular sensors

[0053] Take DON at 0.01-20μg·mL respectively -1 , ZEN at 0.1-50μg·mL -1 、AFB 1 At 0.1–3.5 μg·mL -1 range of true toxins, and DON+ZEN (0.01+5,0.5+10,1+20,10+40, 20+50μg·mL -1 ),DON+AFB 1 (0.01+2.5,0.5+2.8,1+2.9,10+3,20+3.1μg·mL -1 ),ZEN+AFB 1 (5+2.5,0+2.8,20+2.9,40+3,50+3.1μg·mL -1 ),DON+ZEN+AFB 1 (0.01+5+2.5,0.5+10+2.8,1+20+2.9,10+40+3,20+50+3.1μg·mL -1 ) to stimulate the sensor and measure the corresponding impedance value.

[0054] 2. Analytical conditions and methods

[0055] Under the condition of the frequency of 1Hz ~ 100kHz, the reaction medium liquid is 2.5mmol L -1 Fe(CN)6 3- / 4- The measurement was carried out in the solution, using the EIS method, and the impedance value was fitted by Zview software and calculated by the best equivalent circuit.

[0056] Inhibition rate (%) = 100 [(R 鼠尾胶原 -R 真菌毒素毒素 ) / (R 细胞 -R 层粘连蛋白 )]

[0057] R 层粘连蛋白 : ...

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Abstract

The invention discloses a cell electrochemical sensor for analyzing joint toxicity of fungaltoxin, belonging to the fields of analysis and detection of food quality. According to the cell electrochemical sensor, the sensitivity of the sensor is increased by virtue of electroplating gold nanoparticles, an in-vivo physiological living environment of cells is simulated by virtue of laminin, and a three-dimensional model is constructed by virtue of rat tail collagens so as to relatively truly reappear cell morphology; and the constructed cell electrochemical sensor can rapidly and sensitively respond to the influences caused by the fungaltoxin to human hepatoma carcinoma cells, equipment is convenient and rapid and low in production cost, and by combining with an electrochemical resistance value and a joint index method, the type and degree of a joint action of the fungaltoxin can be rapidly judged. The constructed portable cell electrochemical sensor is successfully applied to the toxicity analysis of the fungaltoxin in foods and has good application prospects in the analysis of the joint toxicity of the fungaltoxin.

Description

technical field [0001] The invention relates to a cell electrochemical sensor for analyzing combined toxicity of mycotoxins, which belongs to the field of food quality analysis and detection. Background technique [0002] Mycotoxins are a group of secondary metabolites with different chemical properties produced by some toxin-producing fungi during grain field growth, harvesting and storage, or during feed processing and storage, which can easily cause pathological changes and physiological lesions in humans and animals. At present, food and feed are seriously polluted by mycotoxins. The annual loss of food and feed due to fungal contamination accounts for 20%-30% of the total production in the world, and 25% of grains are polluted by mycotoxins every year, causing huge economic losses. At present, there are more than 200 kinds of mycotoxins that can cause human and animal poisoning, mainly Fusarium, Aspergillus and Penicillium. Deoxynivalenol (DON), zearalenone (ZEN) and a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/27G01N27/327G01N27/30
CPCG01N27/27G01N27/308G01N27/3277G01N27/3278
Inventor 孙秀兰皮付伟纪剑张银志夏爽孙嘉笛
Owner JIANGNAN UNIV
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