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Gastric cancer detection kit and application using hoxb9 and pbx1 as biomarkers

A technology of HOXB9 and detection reagents, applied in the field of biomedicine, can solve the problems of inability to judge the correlation of markers, low accuracy, large tissue consumption, etc., and achieves good clinical application prospects, high specificity, and good sensitivity. Effect

Active Publication Date: 2018-03-09
SHANGHAI CITY JIADING DISTRICT CENT HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Due to the unknown pathogenesis of gastric cancer, the existing gastric cancer-related markers are not effective in clinically judging the growth and prognosis of gastric cancer
The detection of each gastric cancer marker requires at least one slice, which consumes a lot of tissue, and the correlation between markers cannot be judged, and the accuracy is not high

Method used

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  • Gastric cancer detection kit and application using hoxb9 and pbx1 as biomarkers
  • Gastric cancer detection kit and application using hoxb9 and pbx1 as biomarkers
  • Gastric cancer detection kit and application using hoxb9 and pbx1 as biomarkers

Examples

Experimental program
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Effect test

Embodiment 1

[0045] Example 1. Detection of the expression level and location of HOXB9 and PBX1 in gastric cancer tissue by immunofluorescence double staining

[0046] 1. In this example, the expression level and location of HOXB9 and PBX1 in gastric cancer tissue were detected by immunofluorescence double staining, and the steps were as follows:

[0047] (1) Gastric cancer tissues were collected by endoscopy or surgically removed, fixed in 10% formalin, embedded in paraffin, and sectioned in 5 mm.

[0048] (2) Baked slices: 60 degrees Celsius, 1 hour.

[0049] (3) Dewaxing: 15 minutes in xylene solution I, 15 minutes in xylene solution II, 5 minutes in absolute ethanol I, 5 minutes in absolute ethanol II, 5 minutes in 90% ethanol I, 90% ethanol II 5 minutes in 70% ethanol, 5 minutes in double distilled water, and washed twice.

[0050] (4) Antigen retrieval: high-pressure retrieval method, adding 0.01mol / L sodium citrate (Ph 6.0), heating for 10 minutes and then slowly cooling to room t...

Embodiment 2

[0064] Example 2. Construction and application of a gastric cancer detection kit using HOXB9 and PBX1 as biomarkers

[0065] In this example, a gastric cancer detection kit using HOXB9 and PBX1 as biomarkers was constructed, and the kit included: a reagent for detecting HOXB9 and a reagent for detecting PBX1. A reagent for detecting HOXB9 includes a primary antibody for detecting HOXB9 and a secondary antibody for detecting the primary antibody. A reagent for detecting PBX1 includes a primary antibody for detecting PBX1 and a secondary antibody for detecting the primary antibody.

[0066] Specifically, the gastric cancer detection kit of this embodiment detects the expression levels of HOXB9 and PBX1 in gastric cancer tissue by immunofluorescence double staining. The primary antibody used to detect HOXB9 was a mouse-derived HOXB9 antibody, and the second antibody used to detect the first antibody was a FITC-labeled rabbit-derived antibody. The primary antibody used to detect...

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Abstract

The invention belongs to the technical field of biomedicine, and particularly relates to a gastric cancer detection kit adopting HOXB9 and PBX1 as biomarkers and an application. With the adoption of the detection kit, HOXB9 and PBX1 in a tissue sample are detected jointly, the gastric cancer is diagnosed and detected, and detection results have good sensitivity and high specificity. The kit can be effectively used for early screening and / or prognosis evaluation of the gastric cancer and has good clinical application prospect.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a gastric cancer detection kit and application using HOXB9 and PBX1 as biomarkers. Background technique [0002] Gastric cancer is a common malignant tumor of the digestive tract. Its incidence rate accounts for the fourth of new tumors every year in the world, and its mortality rate ranks the second. The formulation of diagnosis and treatment plan for gastric cancer depends on the results of pathological and molecular pathological diagnosis. Molecular pathology can provide evidence for the diagnosis, differential diagnosis, classification and staging, chemotherapy, targeted therapy and prognosis of gastric cancer with the help of immunohistochemical detection technology. Immunohistochemistry is a technique for in situ detection of the expression and distribution of tumor-associated antigens in tissue cells using labeled specific antibodies. At present, more tha...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/574G01N33/533
CPCG01N33/533G01N33/57446
Inventor 常庆朱正纲陈跃宇何昌玉章立史配东万伯顺梁冬雨晁叶青
Owner SHANGHAI CITY JIADING DISTRICT CENT HOSPITAL
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