Chip and bacterium analysis instrument for food safety detection

A food safety and chip technology, applied in analytical materials, instruments, measuring devices, etc., can solve the problems of low specificity, low sensitivity, low sensitivity, etc., and achieve the effects of enhanced adhesion, high detection sensitivity, and improved adsorption capacity.

Inactive Publication Date: 2012-07-04
THE HONG KONG POLYTECHNIC UNIV
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Problems solved by technology

However, the traditional electrochemical detection device lacks the specificity of bacterial detection, and the sensitivity is low, which greatly hinders the popularization and use of this method. It has the following disadvantages: 1. Low sensitivity: when the size of the metal electrode is reduced, the electrode The electrode impedance caused by polarization increases in the low frequency range. If the impe

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  • Chip and bacterium analysis instrument for food safety detection
  • Chip and bacterium analysis instrument for food safety detection
  • Chip and bacterium analysis instrument for food safety detection

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Embodiment Construction

[0035] The present invention will be described in detail below in conjunction with the manufacturing method of the chip of the present invention.

[0036] figure 1 It is a flowchart of the manufacturing method of the chip of this invention. Such as figure 1 as shown,

[0037] Step 1: Silicon Nitride (Si 3 N 4 ) silicon wafer as the initial material, with one side of the silicon nitride layer as the top of the silicon wafer, and the other side as the bottom.

[0038] Step 2: Etching the silicon nitride layer under the silicon wafer to form openings for the cells.

[0039] Step 3: The silicon layer penetrating the silicon wafer is then etched using potassium oxide (KOH) solution as a wet etchant. Preferably, KOH etches the crystal plane , forming a "V" shaped groove.

[0040] Step 4: A layer of aluminum is sputtered on the silicon nitride layer above the silicon wafer.

[0041] Step 5: Under the condition of direct current and constant voltage, the nanoporous aluminum ox...

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Abstract

The invention discloses a chip and a bacterium analysis instrument for food safety detection by using impedance spectroscopy. The chip is based on a nanometer hole alumina film, modules with different bacterium antibody microarrays are formed on the chip after trusted platform module (TPM) modification, polyethylene glycol (PEG) layer formation and bacterium antibody microarray module formation, and the modules can be used for fast and sensitively detecting various kinds of bacteria in food at the same time. The bacterium analysis instrument disclosed by the invention has the characteristics that convenience and high speed are realized, the portability is good, the operation is simple, and the like, and the bacterium analysis instrument can be used for the field detection of the food safety.

Description

technical field [0001] The invention relates to the technical field of food safety, in particular to a chip and a bacteria analyzer for food safety detection. Background technique [0002] Recent incidents of food contamination have aroused great concern about food safety. The food industry has increasingly stringent regulations on acceptable levels of pathogens. However, traditional food pathogen detection techniques such as enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR) are time-consuming and labor-intensive. In order to solve these problems, people have used indirect inspection methods, such as electrochemical methods, for the rapid detection of pathogens by monitoring the signal changes output by the sensor, and there have been many reports about its application in rapid bacterial detection in the literature. However, the traditional electrochemical detection device lacks the specificity of bacterial detection, and the sensitivity is low,...

Claims

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Application Information

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IPC IPC(8): G01N27/04G01N33/569
Inventor 杨莫梁杏媚于进江
Owner THE HONG KONG POLYTECHNIC UNIV
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