Method for identifying heterophilic antibody interference in antibody microarray system and antibody microarray chip using same for detecting target antigen

Abstract

The invention provides a method for identifying heterophilic antibody interference in an antibody microarray system, which is mainly characterized in that: a heterophilic antibody identification point is increased during manufacturing an antibody microarray chip. The antibody of the identification point consists of analogues of solid phase antibodies of various indexes. The method comprises the following steps of: after samples with statistical significance quantity are detected, establishing judgment criteria of heterophilic antibody positive interference; and judging whether the later clinical samples of the same indexes have the heterophilic antibody positive interference according to the judgment criteria. The method identifies the heterophilic antibodies without increasing the conventional workload and reagents; the method does not need to add a blocking agent into each sample so as to save the consumption of many blocking agents, reduce the cost and reduce unknown interference; and meanwhile, automatic identification and objective judgment can be performed through a biochip reader, and analysis on the clinical symptoms of patients is not needed.

Description

technical field [0001] The invention relates to a method for identifying heterophilic antibody interference in an antibody microarray system and an antibody microarray chip using the same to detect target antigens. Background technique [0002] Analysis of multiple proteins in patient serum to guide diagnosis, treatment, and prognosis has promoted the development of parallel protein analysis methods (Expert Rev Mol Diag 2007; 7:87-98.). This method of parallel analysis of multiple proteins is called protein chip, protein microarray chip, etc. The current multi-index analysis method is mainly based on the traditional immunological principle, immobilizing the antigen or antibody on the solid phase surface in the form of an array, specifically binding to the corresponding target substance in the serum, and combining with the labeled antibody, The markers can be photographed or scanned by the protein chip reader, and the computer software analyzes the scanned or photographed re...

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Problems solved by technology

[0006] An object of the present invention is to overcome the defects that the above-mentioned existing methods cannot completely eliminate the influence of heterophilic antibodies or cannot effectively identify heterophilic antibodies, and provide a method for identifying the interference of heterophilic antibodies in an antibody microarray system

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