37 results about "AIM antigen" patented technology

A method of identifying an antigen-specific regulatory T cell (Treg) from a subject is discussed wherein the method comprises quantitatively or qualitatively detecting co-expression of each of cell markers CD4, CD25 and CD134, or alternatively, N each of cell markers CD8, CD25 and CD137, as well as one or more cell markers selected from the group of Treg cell markers consisting of CD39, CD73, CD127, CTLA-4 and Foxp3 on a cell in a suitable lymphocyte-containing sample from the subject in response to exposure to a target antigen. Also discussed are methods of isolating and expanding the identified antigen-specific Treg population, which may permit antigen-specific Treg cell therapy.

The present invention is directed to novel artificial T helper cell epitopes (Th epitopes) designed to provide optimum immunogenicity when used in peptide immunogens comprising B cell epitopes or peptide haptens, a target antigenic site of a target antigen for eliciting antibodies thereto. The artificial Th epitopes are covalently linked to the target antigenic site and optionally an immunostimulatory sequence to provide effective and safe peptide immunogens.

The invention belongs to the biological field, and specifically relates to a bio-sensitive chip as well as a preparation method and use thereof. The problem to be solved by the invention is that the invention provides the bio-sensitive chip which comprises a silver nano chip, 11-sulfydryl hendecanoic acid fixed on the silver nano chip and an antibody connected with the 11-sulfydryl hendecanoic acid. The invention further provides a preparation method of the bio-sensitive chip. The preparation method comprises the following steps: fixing the 11-sulfydryl hendecanoic acid on the silver nano chip; activating the 11-sulfydryl hendecanoic acid; and connecting the 11-sulfydryl hendecanoic acid with the antibody. The invention further provides use of the bio-sensitive chip. A target antigen protein is detected through specific reaction of the antigen of the antibody by means of the antibody on the bio-sensitive chip.

The invention provides a method for identifying heterophilic antibody interference in an antibody microarray system, which is mainly characterized in that: a heterophilic antibody identification point is increased during manufacturing an antibody microarray chip. The antibody of the identification point consists of analogues of solid phase antibodies of various indexes. The method comprises the following steps of: after samples with statistical significance quantity are detected, establishing judgment criteria of heterophilic antibody positive interference; and judging whether the later clinical samples of the same indexes have the heterophilic antibody positive interference according to the judgment criteria. The method identifies the heterophilic antibodies without increasing the conventional workload and reagents; the method does not need to add a blocking agent into each sample so as to save the consumption of many blocking agents, reduce the cost and reduce unknown interference; and meanwhile, automatic identification and objective judgment can be performed through a biochip reader, and analysis on the clinical symptoms of patients is not needed.

The present invention is directed to novel artificial T helper cell epitopes (Th epitopes) designed to provide optimum immunogenicity when used in peptide immunogens comprising B cell epitopes or peptide haptens, a target antigenic site of a target antigen for eliciting antibodies thereto. The artificial Th epitopes are covalently linked to the target antigenic site and optionally an immunostimulatory sequence to provide effective and safe peptide immunogens.

The present invention relates to method of amplifying a population of antigen-specific memory CD4+ T cells using artificial presenting cells expressing HLA class II molecules. In particular, the present invention relates to a method of amplifying a population of antigen-specific memory CD4+ T cells comprising the steps of i) providing a population of artificial antigen presenting cells consisting host cells that are genetically modified to stably express at least one MHC class II molecule along with at least one accessory molecule ii) loading the population of artificial antigen presenting cells of step i) with an amount of at least one antigen of interest and iii) coculturing the suitable population of a T cells with the population of artificial antigen presenting cells of step ii).

The invention discloses a lung cancer DNA plasmid vaccine which comprises a plasmid vector containing a fusion gene MAGE-NY-ESO of four epitopes of lung cancer antigens MAGE-3 and NY-ESO-1. The fusion gene MAGE-NY-ESO has a nucleotide sequence shown as SEQ ID NO.1; the invention also discloses a preparation method of the DNA plasmid vaccine, wherein, a DNA plasmid containing a coding sequence of a target antigen is used as a new generation of therapeutic vaccines. The vaccine induces and generates an immunoreaction with specificity aiming at the relevant coding antigen in the body and effectively restrains the development and transfer of lung cancer. Therefore, the vaccine is the DNA plasmid vaccine with simple preparation, low cost, safety and reliability as well as the effective treatment of lung cancer.

The invention discloses a method and a device for predicting binding affinity based on antigen and antibody sequences. The method comprises the following steps: acquiring a first amino acid sequence of a target antigen and a second amino acid sequence of a target antibody, determining initial features of M dimensions according to the first amino acid sequence of the target antigen and the second amino acid sequence of the target antibody, and inputting the initial features of the M dimensions into a target feature fusion model to obtain M fusion features, and inputting the M fusion features into the target prediction neural network model to obtain predicted binding affinity parameters. The method can be at least applied to the field of intelligent medical treatment, and solves the technical problems of complex prediction mode and low prediction efficiency of the binding affinity of the antigen and the antibody in the prior art.

The invention discloses an ultra-high-sensitivity fluorescence quenching immunosensor of using human troponins I (cTnI) in serum as target proteins and a detection method. The immunosensor is mainly characterized in that the established immunosensor realizes ultra-high-sensitivity detection on the human cTnI in the human serum. On the basis of a lateral-flow immunity chromatography platform, the fluorescence quenching immunosensor of using the human cTnI as model targets is established in the invention, a detection limit on human IgG is determined as 16.26 fg / mL through calculation, results can be obtained averagely after 6 minutes, and ultra-high-sensitivity POCT fast-immunoassay on the above-mentioned target antigens is realized. The lateral-flow immunity chromatography platform disclosed by the invention realizes preparation of lower cost and a shorter process on the premise of ensuring stability and ultra-high sensitivity.

The invention relates to the technical field of immunity, in particular to a cBIN1 antibody and application thereof. Three CDR regions of a heavy chain of the cBIN1 antibody provided by the invention respectively have amino acid sequences as shown in SEQ ID NO: 1, SEQ ID NO: 2 and SEQ ID NO: 3; and three CDR regions of a light chain respectively have amino acid sequences as shown in SEQ ID NO: 4, SEQ ID NO: 5 and SEQ ID NO: 6. The antibody has good binding capacity with an antigen, and accurate detection of a target antigen can be realized by using the antibody to prepare a reagent.

One aspect of the present disclosure relates to an immunochromatographic detection method for a target antigen in a biological sample, comprising the step of joining a linker having a first antibody and a quantum dot bead having a second antibody, with respect to the target antigen. By using the quantum dot beads and the linker, the method can successfully amplify detection strength and significantly increase detection sensitivity through a simple process without causing a loss of antigens participating in detection when using only quantum dot beads. Furthermore, the present disclosure can significantly amplify detection strength without an additional washing step, thus enabling excellent detection and identification of a physiological substance in a biological sample, even in an actual product, and can be used to provide a product with a competitive price.

There are provided a cartridge for detecting a target antigen and a method for detecting a target antigen existing in a biological sample using the cartridge. In the cartridge, multiple detections of various antigens can be rapidly and conveniently performed, and a plurality of target antigens can be quantitatively analyzed using one cartridge, thereby reducing time and cost.

The invention belongs to the field of analytical techniques and methods, and relates to the preparation of a spectral type, near-infrared electrochemiluminescence (~915nm) immunosensor, which mainly includes (1) using methionine as a stabilizer to prepare a water-soluble Au‑Ag bimetallic nanoclusters, (2) gold-silver bimetallic nanoclusters labeled secondary antibody (Au‑Ag|Ab 2 ), (3) preparation of spectral near-infrared electrochemiluminescence sensor with gold-silver bimetallic nano-gold-silver clusters as markers based on the principle of sandwich immunity, and (4) drawing of the working curve. The detection sensitivity of the prepared spectral electrochemiluminescence immunosensor is high, and other antigenic proteins do not interfere with the sensing and detection of the target antigen of the invention.

The invention discloses a method for obtaining antigen-specific T cells, which comprises the following steps: step 1, collecting peripheral blood, and separating to obtain mononuclear cells PBMC; step 2, obtaining immature DC cells; step 3, stimulating and inducing immature DC cells to be mature; 4, separating and purifying the initial T cells; 5, mixing the mature DC cells and the initial T cells according to the cell quantity ratio of 1: (5-120), adding a target antigen for co-stimulation culture for 1-3 rounds, and continuing to culture for 5-128 hours to obtain a cell suspension; 6, separating and purifying antigen-specific T cells; by adopting the method, the proportion of the antigen T cells in the co-culture cell suspension can be effectively increased.

A binary vector and the provision of expression of a target antigen-dependent regulatory cytokine using the synNotch technology, so as to partially release the cytokine to enhance the function of T cell and reduce the non-specific toxic and side effect of the cytokine. A synthetic notch receptor is used to construct plasmid vectors to achieve local cytokine secretion. One plasmid vector uses a specific antibody as an extracellular domain for identifying a target antigen, a notch core comprises a transmembrane domain and a specific enzyme cutting site on a notch receptor, and transcription factor GAL4VP64 is used as an intracellular domain. When the target antigen is identified, the enzyme cutting site on the notch will be identified by a corresponding enzyme to hydrolyze and cut the intercellular transcription factor GAL4VP64. Another plasmid vector carries DNA identification / binding domain GAL4UAS of transcription factor GAL4 and correspondingly activates a minimal promoter of a target gene, and after being hydrolyzed and cut, GAL4VP64 will be bound to the binding domain to activate transcription of subsequent genes so as to express IL12 and secrete same out of the cell.

The invention relates to the technical field of immunization and particularly relates to an antibody cBIN1 and application thereof. Three CDR regions of a heavy chain of the antibody cBIN1 provided bythe invention separately have amino acid sequences represented by SEQ ID NO: 1, 2 and 3; and three CDR regions of a light chain of the antibody cBIN1 separately have amino acid sequences representedby SEQ ID NO: 4, 5 and 6. The antibody has good binding ability with an antigen, and reagents prepared from the antibody can achieve accurate detection on target antigens.

In one aspect, the present disclosure relates to a quantum dot bead comprising a multifunctional ligand having a first conjugated material and a second antibody, and an immunochromatographic detectionmethod for a target antigen in a biological sample, comprising forming multiple bonds with a quantum dot having a second conjugated material. In addition, the present disclosure has the effect of remarkably amplifying the detection intensity and significantly improving the detection sensitivity without a separate washing step, and thus enables the detection and diagnosis of physiological substances in a biological sample even in an actual product, and may be used to provide a product with excellent price competitiveness.

A chimeric antigen receptor containing a target antigen binding domain, a transmembrane domain and a T cell activation signal transduction domain, wherein the target antigen is ganglioside GM2.

The invention provides a data processing method and related equipment, and the method comprises the steps: querying data stored in a database according to the input data of a client, determining an abnormal problem of the client, carrying out the corresponding operation according to the antibody information stored in the database, and avoiding the continuous impact on the client from the same abnormal data. The method comprises the following steps: monitoring target input data corresponding to a target client; when the target antigen data matched with the target input data is stored in the database, obtaining target antibody information corresponding to the target antigen data, wherein multiple pieces of antigen data including target antigen data and multiple pieces of antibody informationincluding target antibody information are stored in the database, the target antigen data are input data causing abnormality of the target client, and the target antibody information comprises a source, a protocol number, data content and strategy configuration of the target input data; and executing a corresponding operation based on the strategy configuration of the target antibody information.

The invention discloses a label-free ratio fluorescence type antigen detection method. The method comprises the following steps: designing a single-stranded DNA which comprises a recognition DNA sequence for CRISPR and an aptamer of a target antigen, and constructing a component A by spacing the recognition DNA sequence and the aptamer through a plurality of continuous T basic groups; by taking a target antigen as a target, modifying an antibody of the target antigen on a 96-well plate to construct a component B; connecting the surface-carboxylated silicon dioxide microspheres with a DNA chain of which the tail end is modified with amino groups, and complementarily pairing auxiliary DNA with DNA with amino groups to form double chains, so as to construct a component C; adding the component A, the component B, the component C, copper ions, ascorbic acid, DAPI dye and exonuclease III into an object to be detected; and detecting red fluorescence and blue fluorescence of an object to be detected, and detecting the target antigen by a ratio fluorescence method. According to the ratio fluorescence method, the intensities of two kinds of fluorescence are compared to serve as signal parameters, the accuracy is higher, the sensitivity is higher, and the selectivity is better; and moreover, the sensitivity is improved, the defect that a traditional method needs to label an antibody is overcome, and the detection cost is saved.

The invention provides a bispecific antibody and application thereof in cancer treatment, the bispecific antibody comprises a first antigen binding domain specifically bound with a first antigen and a second antigen binding domain specifically bound with a second antigen, the first antigen is CD20, the second antigen is CD3, the first antigen binding domain targeting CD20 can be bound with a target antigen in a high affinity manner, and the second antigen binding domain targeting CD20 can be bound with the target antigen in a high affinity manner. A CD3-targeting second antigen binding domain selects a single-domain antibody structure with medium affinity, so that T cell immune response can be effectively mediated, and the situation that bispecific antibodies are gathered in T cell-enriched tissues such as spleen and lymph nodes in vivo to affect the anti-tumor effect can be prevented; a traditional antibody structural domain with a light chain and heavy chain structure is selected and combined with a single-domain antibody structural domain, so that the in-vivo half-life period of the bispecific antibody can be prolonged, and the anti-tumor activity is improved; in addition, the bispecific antibody provided by the invention can also reduce excessive immune factor secretion in the treatment process, and is helpful for reducing toxic and side effects.

The invention provides a cleaning-free universal ELISA fluorescence immunoassay probe as well as a preparation method and application thereof. The probe is characterized in that through an HBTU / DIEA coupling reaction, carboxyphenylboronic acid is modified onto g-C3N4, after ultrasonic exfoliation, a weak-fluorescence boric acid modified graphite phase carbon nitride nanosheet (BCNNS) can be obtained; an antibody (Ab) is labeled on the BCNNS to obtain a strong-fluorescence Ab-BCNNS composite material (ON), the fluorescence of the Ab-BCNNS composite material is quenched (OFF) along with additionof a target antigen, and the specific detection of the target antigen can be realized based on the ON-OFF phenomenon. And moreover, by changing the labeled antibody in the Ab-BCNNS, the selective detection of different target antigens can be realized.

The invention relates to the technical field of immunity, and particularly relates to a cBIN1 antibody and application thereof. Three CDR regions of a heavy chain of the cBIN1 antibody provided by the invention respectively have amino acid sequences shown as SEQ ID NO: 1, 2 and 3; and three CDR regions of a light chain respectively have amino acid sequences shown as SEQ ID NO: 4, 5 and 6. The antibody has good binding capability with an antigen; a reagent prepared from the antibody can realize the accurate detection on a target antigen.

The embodiment of the invention discloses a recombinant hansenula polymorpha cell disruption buffer solution for expressing a hand-foot-and-mouth disease vaccine antigen as well as a preparation method and application of the recombinant hansenula polymorpha cell disruption buffer solution. The cell disruption buffer solution is prepared from the following components: 1.00 g / L to 100.00 g / L of tromethamine, 10.00 g / L to 100.00 g / L of medicinal sodium chloride and 1.00 g / L to 100.00 g / L of glycerinum. By implementing the method disclosed by the invention, the breakage rate, the target protein content and the target antigen content of the recombinant hansenula polymorpha cell breakage product can be obviously improved.

The invention belongs to the technical field of biomedicine, and discloses a preparation method of an HA (hyaluronic acid) blocking agent and an antibody detection system. The preparation method comprises the following steps of purifying an antibody by ammonium sulfate with concentration of 33%-50%; adding glutaraldehyde into the obtained purified antibody, and crosslinking; after dialysis is completed, recycling the antibody, and storing; mixing a sample, a detection microsphere, a heterotropic antibody interference indication microsphere and a second antibody for marking a detectable signal,so as to form a reaction system; and detecting the detectable signal on the microsphere in a second antibody-antigen-first antibody-microsphere quaternary compound in the reaction system, comparing with a standard value or a standard curve, and determining whether a target antigen exists and / or the number of target antigens in the reaction system. The prepared HA blocking agent has the advantagesthat the anti-interference ability is strong, the HA interference is completely eliminated, the interference to immunity detection is avoided, the occurrence rate of false positive is effectively reduced, and the accuracy and precision rate of immunity detection are improved; the preparation method is simple, the control is easy, and the cost is low.

The present invention relates to the field of immunization technology, in particular to cBIN1 antibody and application thereof. The three CDR regions of the heavy chain of the cBIN1 antibody provided by the present invention have the amino acid sequences shown in SEQ ID NOs: 1, 2 and 3 respectively; the three CDR regions of the light chain respectively have the amino acid sequences shown in SEQ ID NOs: 4, 5 and 3 The amino acid sequence shown in 6. The antibody has good binding ability with the antigen, and the reagent prepared by the antibody can accurately detect the target antigen.

The invention discloses a kit for quantitatively detecting the content of pregnancy-related protein A. The kit comprises a kit body, a sealing cover, a protective cover and a reagent, wherein a solidphase carrier hole, a sample hole, a detection antibody hole, a fluorescein hole, a washing hole and a reading hole are sequentially formed in the kit body; the kit contains all reagent components required for completing immunodetection; the end part of the solid phase carrier loaded in the kit is coated with a capture antibody, and the solid phase carrier is immersed into each hole in the kit sothat the combination of a target antigen in a detected sample and subsequent detection steps can be completed, and the manufacturing and maintenance costs are greatly reduced; the kit is high in detection result accuracy, good in linear relation and high in specificity, and reliable clinical reference value can be provided for early screening of Down's syndrome and early prediction of atherosclerotic plaque rupture.

The invention discloses an antigen-antibody co-display method for screening an antibody library of a membrane antigen. According to the method, a target antigen is membrane protein, is expressed on a cell membrane, and is coupled with an effector structural domain on the inner side of the cell membrane; a specific antibody library aiming at the target antigen is expressed on the surface of a cell membrane through transmembrane peptide, and is coupled with an intracellular effect protein structural domain through the transmembrane peptide; and if the antibody and the antigen on the cell surface can act, the effector structural domain expressed by fusion of the antibody and the antigen can be reassembled into a functional effector, and successful binding of the antibody and the antigen is reported through transcription of a reporter gene. By adopting the technical scheme provided by the invention, the specific antibody aiming at the target antigen can be quickly and effectively screened out.

The invention discloses a method for high-throughput synthesis of a monoclonal antibody stably transfected expression cell strain. The method comprises the following steps: S1, preparing a target antigen and immunizing an animal by adopting the target antigen; S2, after the immunization is completed, taking the animal spleen, screening out memory B cells and plasma cells by adopting a flow cytometry, and preparing a single-cell suspension; S3, carrying out amplification reverse transcription on mRNA of the single cell to synthesize cDNA, amplifying variable regions of a heavy chain and a light chain of an antibody, and respectively connecting the variable regions to a carrier; and S4, carrying out cell transfection to obtain the monoclonal antibody stably transfected expression cell strain. Compared with a traditional hybridoma cell strain, the cell strain prepared by the method disclosed by the invention is stable, the batch-to-batch production of secreted antibodies is stable, the yield is 10-50 times higher than that of the hybridoma cell strain, and the cell strain can be widely applied to industrial production.