Active chemiluminescent protein chip

Abstract

The invention discloses an active chemiluminescent protein chip which comprises a reaction cup, a locking ring, a base and a porous carrier, the porous carrier is coated with an antigen or antibody microarray, an annular tightening part is coaxially arranged at the bottom of the reaction cup, and a plurality of elastic tightening pieces are uniformly distributed on the circumference of the bottom of the annular tightening part; the cup body of the reaction cup is sleeved with the locking ring, and an annular clamping groove is formed in the lower portion of the inner side of the locking ring; the base is of a hollow structure and is vertically through, and the opening end of the bottom of the base is connected with an external pump structure. According to the invention, after a common percolation type color development type protein chip is improved into the active chemiluminescence protein chip, the infiltration time and rate of liquid can be controlled autonomously and accurately, the sensitivity of product detection is improved, and the design and manufacturing requirements of products with higher sensitivity requirements are met.

Description

technical field [0001] The invention belongs to the technical field of immune biology, and in particular relates to an active chemiluminescence protein chip. Background technique [0002] Microarray protein chip technology is a technology that immobilizes specific antigens and antibodies on the surface of a flat carrier in the form of a microarray, and uses the immunological reaction of antigens and antibodies for detection. The existing common technical scheme is to coat the antigen or antibody microarray on the planar carrier, place one or more layers of water-absorbing materials (paper fiberboard, cotton fiber, etc.) under the planar carrier, and then pack it into the chip housing; Add the sample to be tested on the surface of the chip, add the washing solution after the sample penetrates, add the enzyme-labeled antibody after the washing solution penetrates, add the washing solution after the enzyme-labeled antibody penetrates, and finally add the chromogenic substrate, ...

AI Technical Summary

Problems solved by technology

The filter-type protein chip improves the sensitivity and specificity of detection, but there are still some deficiencies in use. Specifically, it controls the movement of the piston through the pump and then controls the flow direction of the liquid. The infiltration time is still in an uncontrollable state, or the control accuracy is still not ideal, and the liquid cannot be effectively removed during the reciprocating movement of the piston. The sample needs to be repeatedly filtered through the chip in the chip chamber, so draining is a relatively difficult task. Obvious problems, which will affect the orderly conduct of subsequent tests

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