Active chemiluminescent protein chip

A technology of chemiluminescence and protein chips, applied in chemiluminescence/bioluminescence, analysis through chemical reactions of materials, scientific instruments, etc. problem, to achieve the effect of improving sensitivity and simple structure

Active Publication Date: 2022-04-05
TAIZHOU SYNO GENE DIGITAL TECH CO LTD
View PDF11 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The filter-type protein chip improves the sensitivity and specificity of detection, but there are still some deficiencies in use. Specifically, it controls the movement of the piston through the pump and then controls the flow direction of the liquid. The infiltration time is still in an uncontrollable state, or the contro

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Active chemiluminescent protein chip
  • Active chemiluminescent protein chip
  • Active chemiluminescent protein chip

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] (1) Fix the nitrocellulose membrane 4 coated with the rubella virus antigen microarray on the base 3, place the cuvette 1 on the base 3 to press the nitrocellulose membrane 4 in advance, and place the locking ring 2 along the The outer wall of the cuvette 1 slides down, and the elastic tightening piece 6 contracts inwardly under the extrusion force from the clamping groove 7. After the top surface is in contact, the elastic tightening piece 6 is tightly attached to the outer wall of the base 3 to grasp the base 3, and the locking ring 2 completes the clamping and fixing work of the cuvette 1 and the base 3;

[0038] (2) Connect the small opening at the bottom of the base 3 to the peristaltic pump;

[0039] (3) Add 200 μL of the sample to be tested into cuvette 1 at room temperature (the sample has been diluted at a ratio of 1:10);

[0040] (4) Turn on the peristaltic pump, set the reaction speed of the peristaltic pump to 0.1 rpm, and the sample to be tested in the cuv...

Embodiment 2

[0047] The difference between embodiment 2 and embodiment 1 is only in step (3), and all the other steps are the same:

[0048] (3) Add 200 μL of the sample to be tested into cuvette 1 at room temperature (the sample has been diluted at a ratio of 1:100);

Embodiment 3

[0050] The difference between embodiment 3 and embodiment 1 is only in step (3), and all the other steps are the same:

[0051] (3) Add 200 μL of the sample to be tested into cuvette 1 at room temperature (the sample has been diluted at a ratio of 1:1000);

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses an active chemiluminescent protein chip which comprises a reaction cup, a locking ring, a base and a porous carrier, the porous carrier is coated with an antigen or antibody microarray, an annular tightening part is coaxially arranged at the bottom of the reaction cup, and a plurality of elastic tightening pieces are uniformly distributed on the circumference of the bottom of the annular tightening part; the cup body of the reaction cup is sleeved with the locking ring, and an annular clamping groove is formed in the lower portion of the inner side of the locking ring; the base is of a hollow structure and is vertically through, and the opening end of the bottom of the base is connected with an external pump structure. According to the invention, after a common percolation type color development type protein chip is improved into the active chemiluminescence protein chip, the infiltration time and rate of liquid can be controlled autonomously and accurately, the sensitivity of product detection is improved, and the design and manufacturing requirements of products with higher sensitivity requirements are met.

Description

technical field [0001] The invention belongs to the technical field of immune biology, and in particular relates to an active chemiluminescence protein chip. Background technique [0002] Microarray protein chip technology is a technology that immobilizes specific antigens and antibodies on the surface of a flat carrier in the form of a microarray, and uses the immunological reaction of antigens and antibodies for detection. The existing common technical scheme is to coat the antigen or antibody microarray on the planar carrier, place one or more layers of water-absorbing materials (paper fiberboard, cotton fiber, etc.) under the planar carrier, and then pack it into the chip housing; Add the sample to be tested on the surface of the chip, add the washing solution after the sample penetrates, add the enzyme-labeled antibody after the washing solution penetrates, add the washing solution after the enzyme-labeled antibody penetrates, and finally add the chromogenic substrate, ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N21/76G01N21/01
Inventor 闫小君王小明潘宝华曾德隆阎平希
Owner TAIZHOU SYNO GENE DIGITAL TECH CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap