The invention discloses a detection method of brexpiprazole starting material related substances. The detection method comprises steps as follows: a, preparation of a reference substance solution; b, measurement of the reference substance solution; c, preparation of a test solution; d, measurement of the test solution. With the adoption of the detection method of brexpiprazole starting material related substances, through screening with multiple processes, impurities A1-1, A1-2, A1-3 and A1-4 in a brexpiprazole starting material 7-hydroxy-2-quinolone can be effectively separated and detected, the specificity is high, detection results are accurate and reliable, and the method has multiple advantages of being high in precision, good in stability, good in repeatability, convenient to operate, shorter in time and the like. Compared with existing methods, the method has the advantages that various impurities of samples can be measured more comprehensively, the detection time is appropriate, the results are more reliable, and the method is suitable for popularization and application.