Determination method of various components in Yupingfeng preparation

Abstract

The invention relates to a content determination method for multiple components in a Yupingfeng preparation. The method comprises the steps of: (1) taking the Yupingfeng preparation, crushing or not crushing it, then performing precise weighing, adding methanol to conduct reflux extraction for 1-3h, carrying out filtration and concentration, then adding methanol to a constant volume, thus obtaining a test solution; (2) precisely weighing the reference substance prim-o-glucosylcimifugin, calycosin-7-glucoside, cimifugin, 5-O-methylvisammioside, sec-o-glucosylhamaudol, calycosin, formononetin, atractylenolide III, atractylenolide I and atractylenolide II respectively, and adding methanol to perform dissolving to a constant volume, thus obtaining a mixed reference solution; (3) conducting determination: precisely sucking the test solution and the mixed reference solution respectively, injecting them into a high performance liquid chromatograph, carrying out gradient elution under certain mobile phase condition, and performing multi-wavelength simultaneous monitoring, thus obtaining the content. The method provided by the invention can accurately determine the content of 10 components in the Yupingeng preparation, and can objectively, comprehensively and sensitively reflect the quality condition of the Yupingfeng preparation.

Description

technical field [0001] The invention relates to the field of medicinal material detection, in particular to a method for determining the content of various components in a Yupingfeng preparation. Background technique [0002] The classic prescription Yupingfeng preparation is a representative prescription for strengthening the body and eliminating evils. It is derived from Zhang Song's "Researching the Original Prescription" in the sixth year of Jiading in the Southern Song Dynasty (1213), and it is composed of Astragalus, Atractylodes macrocephala and Fangfeng. Among them, Astragalus membranaceus is sweet and warm, nourishes the qi of the spleen and lungs inside, and can strengthen the surface and stop sweating externally. It is the monarch drug; Scatter wind evil. This party is like a barrier against external evils to the human body, so it is called Yupingfeng. It has the effects of replenishing qi and solidifying the surface, strengthening the spleen and antiperspirant....

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Problems solved by technology

[0003] However, at present, the quality control method of Yupingfeng preparations still has relatively large defects. For example, the Chinese Pharmacopoeia only characterizes its quality by the content of astragaloside IV obtained through ammonia conversion (the content of astragaloside IV in medicinal materials is relatively low), Other literatures mainly focus on the components of Radix Astragali and Fangfeng in the preparation, while the control on the ministerial drug Atractylodes Rhizoma Atractylodes Rhizome is relatively rare, and content determination is one of the key contents in the quality standards of traditional Chinese medicine. For compound preparations, only the preparation It is difficult to objectively, comprehensively and sensitively reflect the quality of Yupingfeng preparations, that is, it is difficult to fully control the quality of traditional Chinese medicine preparations.

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