Method for rapidly distinguishing production places of jasmine-fragrance grapes based on GC-IMS fingerprint spectrums

A GC-IMS and fingerprint technology, which is applied in the field of quickly distinguishing the origin of jasmine grapes, can solve the problems of time-consuming and complicated processing, and achieve the effect of high sensitivity, good stability, and accurate distinction of origin

Inactive Publication Date: 2022-01-11
SHANGHAI INSTITUTE OF TECHNOLOGY
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

[0003] The technical problem to be solved by the present invention is: the origin traceability methods of Jasmine grapes are mostly analyzed according to elements, and the pretreatment of such methods is complicated and time-consuming

Method used

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  • Method for rapidly distinguishing production places of jasmine-fragrance grapes based on GC-IMS fingerprint spectrums
  • Method for rapidly distinguishing production places of jasmine-fragrance grapes based on GC-IMS fingerprint spectrums
  • Method for rapidly distinguishing production places of jasmine-fragrance grapes based on GC-IMS fingerprint spectrums

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Embodiment 1

[0031] A method for quickly distinguishing the place of origin of jasmine grapes based on GC-IMS fingerprints, comprising the following steps:

[0032] Step 1, get three kinds of jasmine grape samples and carry out HS-GC-IMS determination, and concrete determination conditions are as follows:

[0033] HS-GC: 3.5g sample (peeled jasmine grapes, 3.5g pulp) was placed in a 20mL headspace sampling bottle, incubated at 40°C for 15min, and 100μL was injected into the headspace for testing with a gas-phase ion mobility spectrometer, carrier gas N 2 (purity≥99.999%), injection needle temperature 85°C, chromatographic column FS-SE-54-CB-1 (15m, ID: 0.53mm), stationary liquid film thickness: 1μm, carrier gas flow rate 0~2min, 2m L / min; 2~20min, 2~100mL / min.

[0034] IMS: drift tube length: 98mm; linear voltage inside the tube: 500V / cm; drift tube temperature: 45°C; drift gas N 2 (purity≥99.999%); drift gas flow rate: 150mL / min; radioactive source: β-ray (tritium, 3H); ionization mode...

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Abstract

The invention discloses a method for rapidly distinguishing production places of jasmine-fragrance grapes based on GC-IMS fingerprint spectrums. According to the method, a headspace gas chromatography-ion mobility spectrometry combined instrument is adopted to detect the jasmine-fragrance grapes from different producing areas to obtain GC-IMS sample data, the GC-IMS sample data is subjected to statistical analysis through LAV analysis software and GC * IMS Library Search Software qualitative software of the instrument, and the GC-IMS fingerprint spectrums of the volatile aroma components of the jasmine-fragrance grapes from various producing areas are acquired so as to distinguish the producing areas of the jasmine-fragrance grapes. According to the invention, the GC-IMS technology is adopted to perform collection and trace analysis on the complex aroma substances in the jasmine-fragrance grapes in different production places, so comprehensive fingerprint information is provided for production place distinguishing of the jasmine-fragrance grapes, the rapid and accurate distinguishing of the jasmine-fragrance grapes in different production places is achieved, and the theoretical basis and the data support are provided for the production place identification and the source tracing of the jasmine-fragrance grapes.

Description

technical field [0001] The invention relates to a method for quickly distinguishing the origin of jasmine grapes based on GC-IMS fingerprints, and belongs to the technical field of food analysis and detection. Background technique [0002] Jasmine grapes are produced in many places, and the flavors of jasmine grapes from different places are not the same due to the difference in growth environment. In recent years, the cultivation area and output have developed rapidly, but there is a phenomenon of uneven quality. Fruit aroma can objectively reflect the flavor characteristics and maturity of the fruit, and at the same time directly determine the quality of the fruit and its processed products, significantly affecting its market influence. Consumers' preferences for different flavors are directly related to their sales. The difference in the flavor of different origins is also the reason for the mixed praise and criticism of its flavor. The identification of volatile flavo...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/86G01N27/622
CPCG01N30/02G01N30/06G01N30/8686G01N27/622
Inventor 冯涛孙嘉卿姚凌云宋诗清孙敏王化田侯飞娜
Owner SHANGHAI INSTITUTE OF TECHNOLOGY
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