The invention discloses a
molecular marker, kit and method for distinguishing Heilongjiang
megalobrama terminalis and
megalobrama amblycephala and
hybrid of Heilongjiang
megalobrama terminalis and megalobrama amblycephala, and relates to a
molecular marker and a kit for distinguishing Heilongjiang megalobrama terminalis and megalobrama amblycephala and their
hybrid and an application thereof. According to the invention, primer pairs for distinguishing comprise an HLJFLf2 primer pair and an HLJFLr2 primer pair. The kit disclosed by the invention comprises the
molecular marker primer pair HLJFLf2 and the molecular marker primer pair HLJFLr2. The method comprises the following steps: 1, extracting
genome DNA to be detected, 2, carrying out PCR amplification by taking the extracted
genome DNAas a template and taking HLJFLf2 and HLJFLr2 as primers to obtain a PCR product, and 3, carrying out
electrophoresis detection on the PCR product, wherein the individual corresponding to the
electrophoresis result that only the 249bp band appears is the pure Heilongjiang megalobrama terminalis, the individual only the 335bp band appears is the pure megalobrama amblycephala, and the individual withthe 249bp band and the 335bp band appears is the
hybrid of the Heilongjiang megalobrama terminalis and megalobrama amblycephala.