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Molecular marker KB2 for identifying brassica rapa clubroot resistance and primer and application thereof

An anti-clubroot, molecular marker technology, applied in the determination/inspection of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc. The results are easy to confuse and other problems, so as to achieve the effect of simple and quick detection, accurate distinction and good specificity.

Active Publication Date: 2018-04-20
TIANJIN RES INST OF VEGETABLE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although some molecular markers linked to this site have been published, their linkage is not high enough, and there may be some false positives, or when testing, the difference between resistant and susceptible materials is not obvious, and the results are easy to confuse

Method used

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  • Molecular marker KB2 for identifying brassica rapa clubroot resistance and primer and application thereof
  • Molecular marker KB2 for identifying brassica rapa clubroot resistance and primer and application thereof
  • Molecular marker KB2 for identifying brassica rapa clubroot resistance and primer and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Obtaining F2 Segregation Population of Chinese Cabbage Clubroot Disease-resistant Materials

[0033] The Chinese cabbage clubroot disease-resistant material G6 was used as the female parent, and the susceptible material G4 was used as the male parent to obtain the F1 generation. The plants of the F1 generation were identified as disease-resistant materials by field disease resistance identification. The segregation population of F2 generation obtained by selfing of F1 generation was used for phenotype identification and molecular marker detection of Chinese cabbage clubroot.

Embodiment 2

[0035] Phenotypic Identification and Molecular Marker Detection of Clubroot Disease in F2 Generation Population of Chinese Cabbage

[0036] 1. Phenotypic identification of clubroot resistance in F2 population of Chinese cabbage

[0037] Taking the F2 population obtained in Example 1 as material, inoculate Physiological race No. 4 of Plasmodium phylloxera after sowing, the inoculation and investigation methods refer to the literature "Wang Weihong et al., Identification of Physiological Races of Cruciferous Vegetable Physiological Races and Resistance Source Screening, Chinese Vegetables, 2013 Vol. 1(12): 55-60". The results showed that among the 64 F2 generation Chinese cabbage plants, 47 were resistant and 17 were susceptible ( figure 1 ).

[0038] 2. Molecular marker detection of clubroot resistance in F2 population of Chinese cabbage

[0039]The CTAB method was used to extract the leaf genomic DNA of the clubroot-resistant material G6, the susceptible material G4, and t...

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Abstract

The invention discloses an InDel molecular marker KB2 co-separated with a brassica rapa clubroot resistance gene Crrla, a primer and application thereof. The invention further provides a method for identifying or auxiliarily identifying whether a brassica rapa contains the clubroot resistance gene Crrla or not, and whether the brassica rapa is capable of resisting a clubroot disease or not. The method comprises the steps that a to-be-detected brassica rapa genome serves as a template, the primer provided by the invention is subjected to PCR amplification, and whether the to-be-detected brassica rapa contains the clubroot resistance gene Crrla or not and whether the brassica rapa can be capable of resisting the clubroot disease or not are judged according to the size of an amplification product. The molecular marker is utilized to conduct auxiliary selection on the clubroot resistance brassica rapa, the accuracy is high, the cost is low, and both time and labor are saved.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a molecular marker KB2 for identifying the resistance to clubroot of Chinese cabbage, a primer and an application thereof. Background technique [0002] Chinese cabbage ( Brassica rapa L. ssp. pekinensis ) belongs to the Brassicaceae genus, and is one of the vegetable crops with the largest planting area and output in my country. Clubroot is caused by the clubroot fungus ( Plasmodiophora brassicae Woronin), which can cause abnormal swelling and nodules in the roots of cruciferous plants such as Chinese cabbage, is a serious worldwide root disease of cruciferous plants, which can cause global The 10%-15% reduction in production, in areas with severe disease, even leads to complete production, which seriously threatens the production of cruciferous vegetables. The disease was first discovered in Europe. In recent years, there have been reports on the occurrence of clubroot in mo...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 张斌张红闻凤英刘晓晖温娟娟华德平
Owner TIANJIN RES INST OF VEGETABLE
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