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Acer truncatum bunge rapid seedling method

A technology of Yuanbaofeng and sterile seedlings, applied in horticultural methods, botanical equipment and methods, planting substrates, etc., can solve the problems of low survival rate, long cycle, and low rooting rate of Yuanbaofeng seedlings, so as to simplify the breeding process, The effect of high rooting rate and high promotion and application value

Pending Publication Date: 2020-09-15
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the problems of low rooting rate, low survival rate and long period of seedling cultivation of Maple indica existing in the prior art, the present invention provides a method for rapidly growing seedlings in one step in a test tube of twigs and stems of maple ingot

Method used

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  • Acer truncatum bunge rapid seedling method
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  • Acer truncatum bunge rapid seedling method

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Effect test

Embodiment 1

[0028] A method for one-step rapid seedling growth in a test tube of the twig stem section of the Yuanbao maple, comprising the following steps:

[0029] 1. Obtaining sterile vaccines:

[0030] 1) Material selection: Collect the branches of the maple in the same year as the source of explants, cut them into 2-3cm budded stem segments, and use them as explants for later use;

[0031] 2) Explant treatment: Wash the surface of the explant with washing powder solution for 2 minutes, the concentration of the washing powder solution is 0.05%, and wash with sterilized water until the washing powder solution is eluted completely. On the ultra-clean workbench, add 75% alcohol to disinfect the surface for 30 seconds, remove the alcohol, and add HgCl with a mass-volume ratio of 0.1% 2 , sterilize for 2-4min, shake to make HgCl 2 The solution is in full contact with the explants to ensure thorough disinfection, remove the disinfectant, wash 4-5 times with sterile water, place it on ster...

Embodiment 2

[0040] A method for one-step rapid seedling growth in a test tube of the twig stem section of the Yuanbao maple, comprising the following steps:

[0041] 1. Obtaining sterile vaccines:

[0042] 1) Material selection: Collect the branches of the maple in the same year as the source of explants, cut them into 2-3cm budded stem segments, and use them as explants for later use;

[0043] 2) Explant treatment: firstly, the surface of the explant was washed with washing powder solution for 2 minutes. The concentration of the washing powder solution was 0.05%, and washed with sterilized water until the washing powder solution was eluted completely. On the ultra-clean workbench, add 75% alcohol to disinfect the surface for 30 seconds, remove the alcohol, and add HgCl with a mass-volume ratio of 0.1% 2 , sterilize for 2-4min, shake to make HgCl 2 The solution is in full contact with the explants to ensure thorough disinfection, remove the disinfectant, wash 4-5 times with sterile wate...

Embodiment 3

[0056] A method for one-step rapid seedling growth in a test tube of the twig stem section of the Yuanbao maple, comprising the following steps:

[0057] 1. Obtaining sterile vaccines:

[0058] 1) Material selection: Collect the branches of the maple in the same year as the source of explants, cut them into 2-3cm budded stem segments, and use them as explants for later use;

[0059] 2) Explant treatment: firstly, the surface of the explant was washed with washing powder solution for 2 minutes. The concentration of the washing powder solution was 0.05%, and washed with sterilized water until the washing powder solution was eluted completely. On the ultra-clean workbench, add 75% alcohol to disinfect the surface for 30 seconds, remove the alcohol, and add HgCl with a mass-volume ratio of 0.1% 2 , sterilize for 2-4min, shake to make HgCl 2 The solution is in full contact with the explants to ensure thorough disinfection, remove the disinfectant, wash 4-5 times with sterile wate...

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Abstract

The invention relates to an acer truncatum bunge rapid seedling method, in particular to an in-vitro one-step rapid seedling method of acer truncatum bunge epicormic branch stem sections, and belongsto the technical field of plant rapid propagation. The method provided by the invention comprises the steps of collecting acer truncatum bunge annual epicormic branches; cutting stem sections with buds to be used as explants; after sterilization, inoculating the explants onto an explant germination culture medium so that leaves spread from bud bodies to obtain aseptic seedlings; putting new stem sections of the aseptic seedlings onto a rooting culture medium for directly rooting and seedling; and then, performing transplantation. Acer truncatum bunge tissue culture seedlings obtained by the method provided by the invention are healthy, strong and has a high rooting rate. The rooting rate can reach 85 percent; the plant root systems are exuberant; the transplanting survival rate is as highas 98 percent or higher; in addition, the operation is simple; the cost is low; and the method belongs to an efficient, simple and convenient acer truncatum bunge in vitro propagation technical methodwith a very high popularization and application value.

Description

technical field [0001] The invention belongs to the technical field of plant rapid propagation, and more specifically relates to a one-step rapid seedling growth method in a test tube of a young twig stem section of the Chinese maple ingot and an improved medium involved therein. Background technique [0002] Acer truncatum Bunge, also known as Acer truncatum, Acer flat base, and Yuanbao tree, is a small deciduous tree of the genus Acer in the family Aceraceae, with a height of 8-13m. It is mainly distributed in the Yellow River Basin, Northeast my country, Inner Mongolia, Jiangsu and Anhui in China. As one of the main tree species for landscaping in my country, besides its important ornamental value, it is also a multi-purpose woody oil tree species. The seed kernel contains about 48% oil, 25%-27% protein, and a single seed coat. Ning can reach 60%, and the leaves contain flavonoids, chlorogenic acid, cardiac glycosides and other active substances; more importantly, the fat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G24/28A01G24/15A01G31/00
CPCA01H4/008A01H4/001A01G24/28A01G24/15A01G31/00
Inventor 马秋月李倩中李淑顺闻婧朱璐颜坤元李淑娴
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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