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Method for improving rooting ability of pinus massoniana based on endogenous hormones

A technology of endogenous hormones and rooting ability, applied in horticultural methods, botany equipment and methods, plant regeneration, etc., can solve problems such as difficulty in rooting of subculture shoots, large differences in plant regeneration efficiency, and poor physiological activity

Active Publication Date: 2020-09-15
GUANGXI FORESTRY RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, a large number of studies have shown that in the long-term subculture of plants, the vegetative body degenerates, the physiological activity becomes worse, and the regenerative reproduction ability is weakened.
Since 2014, the applicant has successively established 257 aseptic propagation systems of masson pine genotypes. During continuous research and observation, it was found that after 15-20 subcultures (1.5-2 years), the reproduction efficiency of subcultured buds High, the rooting effect is stable, but when the subculture time reaches about 4 years, it is difficult for the subculture buds to take root, the rooting rate and the number of roots are significantly reduced, and the plant regeneration efficiency is low, which hinders the industrialization of masson pine clone seedling cultivation
According to previous studies, the number of subcultures and plant hormones are important factors that lead to large differences in plant regeneration efficiency in subculture

Method used

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  • Method for improving rooting ability of pinus massoniana based on endogenous hormones
  • Method for improving rooting ability of pinus massoniana based on endogenous hormones
  • Method for improving rooting ability of pinus massoniana based on endogenous hormones

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Present embodiment is the method that the endogenous hormone of Pinus massoniana is researched, specifically as follows:

[0024] 1. Experimental materials:

[0025] Taking Tongmian pine as the research object, the national excellent masson pine geographic provenance, the 5 dominant tree method was used to select the best stand in the 15-30-year-old Tongmian pine stand in the state-owned Paiyangshan Forest Farm in Guangxi. The new twigs of the selected excellent trees in the same year were used as spikes, and the half-year-old masson pine tissue culture seedlings were used as rootstocks, and were grafted according to conventional methods. After the grafting survived, remove the top shoots of the grafted seedlings, and use 500mg·L -1 GA3 was sprayed every other week to promote germination, and sprayed 3 to 4 times in a row. When the grafted seedling sprouts grow to 1cm, spray once a week with 0.5% urea, and when the sprouts reach 3-5cm in length, pick vigorous sprouts ...

Embodiment 2

[0036] According to the statistical analysis of Example 1, the applicant found that the ratio of IAA / GAs has a certain influence on the rooting of subcultured shoots. Selecting the rooting agent according to the above ratio can accurately match the content of the rooting agent, greatly improving the rooting ability of the subcultured buds. Application In the long-term study of human beings, it was found that for the explant of the secondary shoot of Pinus massoniana:

[0037] The endogenous hormone 7≤IAA / GAs≤8 of the subcultured shoots of Pinus massoniana subcultured for 1-10 times;

[0038] The endogenous hormone IAA / GAs of the subcultured shoots of Pinus massoniana subcultured for 11-20 times>8;

[0039] The endogenous hormone IAA / GAs of the subcultured shoots of Pine massoniana subcultured 21-40 times<7;

[0040] Among them, the number of subcultures with the most significant differences in rooting rate, root number, and endogenous hormone levels was (1 time: 35-40d, 20 ti...

Embodiment 3

[0065] In the long-term research work, the applicant found that, in addition to the above-mentioned rooting agents, the rooting agents abscisic acid (ABA), forchlorfenuron (CPPU) and isopentenyl adenine (iP) also have an effect on the secondary buds of Pinus massoniana. Great impact; there is a significant difference in the concentration of the first, second and third generation buds, so the applicant carries out further research on the concentration of ABA, CPPU and iP respectively on the first, second and third generation buds, For details, see Table 6-11:

[0066] Table 6 rooting agent is to the orthogonal experiment factor level of first subgeneration bud

[0067]

[0068]

[0069] Table 7 Orthogonal test results

[0070] Number of trials Factor A Factor B Factor C Rooting rate (%) Number of roots 1 1 1 1 92.5±0.8a 8.3±1.0a 2 1 2 2 95.3±1.2a 8.1±0.4a 3 1 3 3 75.6±1.7c 5.3±0.5c 4 2 1 2 97.6±0.3a 7.9±0.4a 5 ...

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Abstract

The present invention relates to the technical field of tissue culture biology and particularly relates to a method for improving rooting ability of pinus massoniana based on endogenous hormones. By setting a ratio of IAA / Gas, successor buds of the pinus massoniana are classified, by typing-selecting different rooting agents of the successor buds, precise addition of the rooting agents is realized. The method effectively improves the rooting ability of the successor buds of the pinus massoniana. The method can rapidly screen out the rooting agents that meet growth conditions of the successor buds, effectively improves the rooting ability of the successor buds of the pinus massoniana, and provides strong scientific and technological supports for accelerating breeding and utilization of excellent pinus massoniana clones.

Description

【Technical field】 [0001] The invention relates to the technical field of tissue culture, in particular to a method for improving the rooting ability of Pinus massoniana based on endogenous hormones. 【Background technique】 [0002] Masson pine (Pinus massoniana) is a native afforestation dual-purpose tree species in subtropical regions of my country. As a tree species with high comprehensive utilization value and broad promotion prospects, masson pine can not only be used to produce three-board, papermaking and chemical fiber industrial manufacturing, but also can be used to produce industrial raw materials such as rosin and turpentine. Due to the large differences in the provenance and family genetic differentiation of Pine masson, the productivity of Pine masson plantations in the south is generally not high, which seriously restricts the rapid development of Pine masson industry. The clonal cultivation through clonal rapid propagation technology is an important way to rea...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 姚瑞玲王胤
Owner GUANGXI FORESTRY RES INST
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