A kind of in vitro plant regeneration method of adult excellent single plant of Milaopai

Abstract

The invention discloses a vitro-plant regeneration method of adult quality single plants of mytilaria laosensis lecomte, belonging to the technical field of tissue culture of plants. The method is that a basic culture medium is provided; different hormone components and concentration level and ratio are optimized; the sprouts at the base parts of the adult quality plants are used as explants and are processed by axillary bud induction, axillary bud proliferation and rooting culture so as to obtain complete plants; after transplanting to the medium, the plants can grow into strong nursery stocks which grow uniformly and have the consistent appearance and shape; the afforesting survival rate is high, and the forest form is uniform. With the adoption of the method, the problems of high pollution rate, slow elongation growth in proliferation culture, low proliferation rate and low rooting rate during sterile construction of mytilaria laosensis lecomte explants can be solved; the seedlings can be quickly cultured in a large scale, and the produced mytilaria laosensis lecomte seedlings grow strongly and uniformly; the production cost is low; the technological support and quality seed guarantee are provided to achieve cloning popularization of the adult quality single plants of mytilaria laosensis lecomte and increase the economic benefits of artificial forests of mytilaria laosensis lecomte; therefore, the vitro-plant regeneration method has good application prospect.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a method for regenerating an isolated plant of an adult fine rice plant. Background technique [0002] Mytilaria laosensis Lecomte belongs to the genus Mytilaria of the family Hamamelidaceae. The adult tree can reach 30m in height and 80cm in diameter at breast height. Rice Lao Pai is naturally distributed in Guangdong, Guangxi, Yunnan, Vietnam, Laos and other places in my country, and it is also artificially cultivated in Fujian and Jiangxi in China. [0003] Milaopai wood is reddish-brown, fine wood structure, straight grain, resistant to dry and wet changes, easy wood processing, good color, beautiful pattern, good brightness after painting, easy to glue, suitable for making furniture, plywood, etc. Its dry shape is straight, not susceptible to insects, corrosion-resistant, and can be used as building materials; Milaopai's wood fiber length is 2112.8u...

AI Technical Summary

Problems solved by technology

[0008] There are few reports on the tissue culture techniques of Milaopai, and there are the following deficiencies: ①The female parent material used for tissue culture is a single plant in a young forest that is 3 to 5 years old, not an adult tree that has been selected for a long time

②The contamination rate is high during explant induction culture, and the establishment efficiency of the sterile line is low; ③The proliferation rate is low (the multiplication rate is only 1.6), the growth of the proliferation buds is slow, the quality of the buds is poor, and the height of each cluster of proliferation buds reaches 1.0cm Buds up to 2.6 plants

④ The rooting rate is not high (less than 90%), the quality of rooted seedlings is poor, the height of the seedlings is only 1.2cm, and it is difficult to transplant and manage

⑤Using this technology for tissue culture and rapid propagation, the speed is slow, the cost is high, and it is difficult to achieve large-scale

It has been verified that using this tissue culture program to tissue culture the shoots at the base of the adult Milaopai tree, the buds cultured by proliferation slow elongation, poor quality, low multiplication rate, low rooting rate, and unstable technology

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