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A method for high-efficiency in vitro plant regeneration of a single adult "Little Joe" cherry of Oshima cherry

A mature and excellent technology for cherry blossoms, applied in the directions of plant regeneration, botanical equipment and methods, horticultural methods, etc., can solve the problems of difficulty in establishing a sterile line of reproduction, complicated rooting culture process, long subgeneration cycle, etc., and achieve high rooting rate. , the effect of short generation cycle and fast induction speed

Active Publication Date: 2017-12-29
SOUTH CHINA AGRI UNIV +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to solve the existing tissue culture technology of 'Xiao Qiao' cherry in the existing tissue culture technology of adult excellent tree tissue culture and propagation aseptic line establishment difficulty, the bud elongation of multiplication culture is slow, the quality is poor, the vitrification phenomenon is serious, the subculture cycle Long, complicated rooting culture process, low rooting rate, technical instability and other technical problems, and provide a high-efficiency in vitro plant regeneration method for a single plant of Oshima cherry adult fine 'Xiaoqiao' cherry, which has the advantages of fast adventitious bud induction, High multiplication rate, strong buds and fast elongation, high rooting rate, developed and robust root system of rooted seedlings, transplanting survival rate, etc.

Method used

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  • A method for high-efficiency in vitro plant regeneration of a single adult "Little Joe" cherry of Oshima cherry
  • A method for high-efficiency in vitro plant regeneration of a single adult "Little Joe" cherry of Oshima cherry
  • A method for high-efficiency in vitro plant regeneration of a single adult "Little Joe" cherry of Oshima cherry

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Experimental program
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Effect test

Embodiment 1

[0031] A high-efficiency in vitro plant regeneration method for an adult and excellent single 'Little Joe' cherry of Oshima cherry, comprising the following steps:

[0032] (1) Collection of explants: At about 10 am when the weather is fine from March to May, cut half-grown, healthy and pest-free cherry trees from the mother plant of 'Xiao Qiao' (adult excellent tree over ten years old). The lignified shoots were taken back to the laboratory after moisturizing treatment, and the collected semi-lignified shoots were removed from the leaves, washed with pure water and stored as explants in a refrigerator at 4°C for later use. Before collecting the explants, the shoots were alternately sprayed with carbendazim and chlorothalonil for 4-5 times every 10 days.

[0033] (2) Surface disinfection of explants: cut branches (explants) into about 4cm long axillary stem segments with leaves, wash them with sterile water on the ultra-clean workbench, and then use 0.5% Xinjier with mass frac...

Embodiment 2

[0043] A high-efficiency in vitro plant regeneration method for an adult and excellent single 'Little Joe' cherry of Oshima cherry, comprising the following steps:

[0044] (1) Collection of explants: From March to May, at about 10 am when the weather is fine, cut semi-woody semi-woody trees that are healthy and free from diseases and insect pests from the mother plant of 'Xiaoqiao' cherry tree (an excellent tree more than ten years old). The new shoots were transformed, and after moisturizing treatment, they were taken back to the laboratory. The collected semi-lignified shoots were removed from the leaves, cleaned with pure water, and then used as explants and stored in a refrigerator at 4°C for later use. Before collecting the explants, the shoots were alternately sprayed with carbendazim and chlorothalonil for 4-5 times every 10 days.

[0045] (2) Surface disinfection of explants: cut the branches (explants) into about 4cm long axillary stem segments with leaves, wash them...

Embodiment 3

[0055] A high-efficiency in vitro plant regeneration method for an adult and excellent single 'Little Joe' cherry of Oshima cherry, comprising the following steps:

[0056] (1) Collection of explants: From March to May, at about 10 am when the weather is fine, cut semi-woody semi-woody trees that are healthy and free from diseases and insect pests from the mother plant of 'Xiaoqiao' cherry tree (an excellent tree more than ten years old). The new shoots were transformed, and after moisturizing treatment, they were taken back to the laboratory. The collected semi-lignified shoots were removed from the leaves, cleaned with pure water, and then used as explants and stored in a refrigerator at 4°C for later use. Before collecting the explants, the shoots were alternately sprayed with carbendazim and chlorothalonil for 4-5 times every 10 days.

[0057] (2) Surface disinfection of explants: cut branches (explants) into about 4cm long axillary stem segments with leaves, wash them wit...

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Abstract

The invention discloses an efficient in-vitro plant regeneration method of an adult high-quality single-plant Xiaoqiao oriental cherry of cerasus lannesiana var. speciosa. According to the efficient in-vitro plant regeneration method, a basic culture medium is set, and different hormone components and concentration level ratios are optimized; annual young sprouts of the adult plant are used as explants, and complete plants are formed by axillary bud induction, axillary bud multiplication and rooting culture; and after the complete plants are transplanted to a base material, healthy and strong nursery stocks which grow orderly and have consistent appearance are obtained. The efficient in-vitro plant regeneration method has the advantages that the growth speed of axillary buds of the explants is high; the induction rate is high and is up to be 100%; the multiplication coefficient is great and is up to be more than 4.5; the elongation growth of multiplied buds is rapid and the buds are 3cm-4cm high after being cultured for 15 days; the rooting rate can be up to be 100% and each plant has more than 5 roots; and tissue culture rooting seedlings are healthy and strong and have high transplanting survival rate. In actual production and application, annual large-scale rapid seedling growing can be carried out to produce young Xiaoqiao oriental cherry seedlings which are healthy and strong, and ordered and consistent, so that the efficient in-vitro plant regeneration method has a wide application prospect.

Description

Technical field: [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a high-efficiency isolated plant regeneration method of a single adult cherry 'Little Joe' cherry. Background technique: [0002] Oshima Sakura (Cerasus lannesiana var. speciosa) belongs to the Rosaceae (Rosaceae) Sakura (Cerasus), native to Japan, mainly distributed in the Izu Peninsula. Oshima cherry is not only a good ornamental plant, but also a traditional food. Its leaves have a special aroma and are a traditional food material loved by the Japanese. The market is in short supply, and it is hoped that the imported cherry leaves can meet the needs of the domestic market. In view of the economic value of Oshima cherry, Foshan City Forestry Research Institute and Dujiangyan City, Guangdong have successively introduced and cultivated it from Japan, and used it for garden viewing and harvested leaves to be processed into semi-finished products and sold...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/005A01H4/008
Inventor 邓小梅叶小玲胡晓敏奚如春朱军郑珂媛张晓明
Owner SOUTH CHINA AGRI UNIV
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