The invention provides a nutritional cucumber seedling raising medium and a preparation method thereof. The nutritional cucumber seedling raising medium is prepared from, by weight, 20-50 parts of turf, 10-30 parts of deep-layer soil medium, 10-20 parts of plant ash, 10-20 parts of animal excrement, 5-10 parts of vermiculite, 10-20 parts of urea, 10-20 parts of weather coal, 10-20 parts of acrylate-acrylamide crosslinking copolymer, 2-8 parts of superphosphate, 1-3 parts of potassium sulphate, 1-3 parts of rooting powder, 5-10 parts of perlite, 5-10 parts of papermaking black liquor alkali lignin fertilizer and 3-8 parts of wheat stubbles. The nutritional cucumber seedling raising medium is low in production cost, safe in seedling raising, fast and easy to manage, convenient to operate, simple and easy to understand and wide in application prospect.