The invention discloses a
virus-free
tissue culture and rapid propagation method of Olea europaea. The method comprises the following steps: 1, removing seed shells: removing seed shells through a sanding technology; 2, making explants: immersing the shelled seeds in a
sucrose solution for 3-5h, taking out the immersed seeds,
drying the immersed seeds in a dark place, and disinfecting the dried seeds; 3, carrying out inductive culture: inoculating the explants to an inductive culture medium to form
callus tissues, transferring the
callus tissues to a differentiation culture medium, and inducing clumpy buds to form rootless tissue seedlings; 4, carrying out propagation culture: inoculating the rootless tissue seedlings to a propagation medium, and carrying out subculture; 5, carrying out rooting culture: inoculating the rootless
tissue culture seedlings to a rooting culture medium, and carrying out rooting culture; 6, carrying out
seedling strengthening culture: carrying out
seedling strengthening culture on the rooted
tissue culture seedlings in a
seedling strengthening culture medium for 10d; and 7,
transplanting: tansplanting the seedlings after the
virus-free tissue culture seedlings grow to 3-5cm, and culturing the transplanted seedlings to form young plants. The method is a new method for growing Olea europaea seedlings, accelerates the seedling growing speed, and meets scale production requirements.