Tissue culture and rapid propagation method of Donghong actinidia fruits

Abstract

The invention discloses a tissue culture and rapid propagation method of Donghong actinidia fruits, and relates to a actinidia fruit variety tissue culture and rapid propagation method. The method includes the steps: (1) aseptic seedling acquisition; (2) adventitious bud regeneration induction; (3) adventitious bud proliferation and strong seedling culture; (4) rooting culture; (5) transplanting.The method is characterized by a germination culture medium, a regeneration culture medium, a proliferated strong seedling culture medium and a rooting culture medium. The four targeted culture mediaare used for tissue culture of the Donghong actinidia fruits, a large number of Donghong actinidia fruit seeds and seedlings can be rapidly propagated in a short time, seed and seedling quality can bemaximally ensured by tissue culture, seed and seedling propagation speed is increased, industrial production of the seeds and seedlings is realized, and the requirements of production for the Donghong actinidia fruit seeds and seedlings are met. Variety seeds and seedlings are directly subjected to tissue culture, a variety cutting orchard is built, the bottleneck of scion grafting in traditionalgrafting propagation is broken, and production requirements are sufficiently met.

Description

technical field [0001] The invention relates to a tissue culture and rapid propagation method of kiwifruit varieties, in particular to a tissue culture and rapid propagation method of kiwifruit Donghong. Background technique [0002] The fruit of Donghong kiwifruit is long and cylindrical, with an average fruit weight of 70-75 grams. The flesh is golden yellow, and the heart is bright red; the flesh is fresh and tender, the juice is medium, the flavor is sweet, the aroma is rich, and the mineral nutrients are rich, especially potassium (2600mg / kg) and calcium (446mg / kg), and the calcium content of the fruit is high. Good for storage. With the acceleration of the selection and breeding of new kiwifruit varieties in China, in order to quickly popularize the new varieties and retain the good quality of the new varieties, the use of kiwifruit tissue culture technology for propagation is one of the fastest and most effective methods. At present, the industrialization of Donghong...

AI Technical Summary

Problems solved by technology

With the acceleration of the breeding of new varieties of kiwifruit in China, in order to quickly promote new varieties and retain the good quality of new varieties, the use of kiwifruit tissue culture technology for reproduction is one of the fastest and most effective methods. At present, the industrialization promotion of Donghong kiwifruit Planting is in the stage of trial implementation. The source of seedlings for large-scale planting is mainly grafted seedlings. The method of tissue culture Donghong seedlings has not been reported yet. The way to obtain seedlings by grafting is the degree of affinity between scion and rootstock and the healing of grafting joints. The degree of grafting directly affects the survival rate of grafting and the growth vigor and high yield of later varieties of scions. In addition, the limitation of the quality and quantity of scions has also become a bottleneck for the large-scale promotion and industrial production of Donghong.