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Tissue culture bud propagation method formertensia maritime

A technology of oyster leaves and tissue culture, applied in the field of cultivation and reproduction of oyster leaves, can solve the problems of artificial planting, difficulty in planting and reproduction of oyster leaves, and low germination rate, and achieves rapid seedling raising, overcoming difficulty in reproduction and long-term vegetative growth of plants. Aging, to achieve the effect of annual cyclical production

Pending Publication Date: 2021-10-26
FUJIAN SANAN SINO SCI PHOTOBIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] For this reason, need to provide a kind of oyster leaf tissue culture bud propagation method, solve existing domestic oyster leaf planting and propagation difficulty, artificial planting, the problem of planting germination rate low

Method used

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  • Tissue culture bud propagation method formertensia maritime
  • Tissue culture bud propagation method formertensia maritime
  • Tissue culture bud propagation method formertensia maritime

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1: the culture of oyster leaf aseptic bud, and explant is carried out optimization,

[0022] (1) Orthogonal experiment with four factors and three levels was carried out on explants, culture medium, 6-BA and NAA.

[0023] Four factors: explant, medium, 6-BA, NAA.

[0024] Three levels: explant selection 1 (tiller bud), 2 (leaf), 3 (seed) 3 different parts; medium selection 1 (MS), 2 (improved MS), 3 (B5) 3 different parts Medium; 6-BA chooses 1 (0.5mg / L), 2 (1.0mg / L), 3 (1.5mg / L) 3 different concentrations; NAA chooses 1 (0.1mg / L), 2 (0.2mg / L) L), 3 (0.5mg / L) 3 different concentrations. Preliminary screening of suitable explants, medium, 6-BA, NAA was carried out through the above orthogonal design. One treatment of 3 bottles, repeated 3 times.

[0025] (2) Rinse the oyster leaf seeds, leaves, and tiller buds, place them in 75% alcohol for 45 seconds, soak in 0.1% mercuric solution for 45 seconds, and in sterile water for 60 seconds, then inoculate them i...

Embodiment 2

[0030] Embodiment 2: Bud Proliferation Culture

[0031] (1) Get the aseptic buds of oyster leaves obtained by test group 7 in Example 1, carry out branching processing, and then inoculate in different medium formulas+7g / L agar+30g / L sucrose respectively, and 1 tissue culture bottle is connected with 1 See Table 3 for the specific experimental design, cultivate in a culture room at 18°C, light intensity 2000lx, and photoperiod 12h / d, and observe the growth situation regularly.

[0032] Table 2, Bud Proliferation Culture Conditions

[0033] test group Nutrient solution 6-BAmg / L NAAmg / L Culture temperature ℃ YB1 B5 0.5 0.05 24 YB2 B5 0.5 0.1 24 YB3 B5 0.5 0 24 YB4 B5 0.5 0.05 18

[0034] (2) Experimental results

[0035] After 30 days of inoculation, count the number of proliferating buds in each test group, and carry out division processing, and count the multiplication multiple and effective bud number of each test gro...

Embodiment 3

[0039] Embodiment 3: rooting culture

[0040] Table 4, rooting culture treatment table.

[0041] test group Nutrient solution IBA(mg / L) 1 1 / 2B5 0.2 2 1 / 2B5 0.5 3 1 / 2B5 1.0 4 1 / 4B5 0.2 5 1 / 4B5 0.5 6 1 / 4B5 1.0 7 B5 0.2 8 B5 0.5 9 B5 1.0

[0042] Get the aseptic seedling that YB4 test group obtains among the embodiment 2, carry out branching processing in ultra-clean workbench, then inoculate respectively in different culture medium formula+7g / L agar+30g / L sucrose, 1 tissue culture bottle connects 1 bud, the specific experimental design is shown in Table 4, cultivated in a culture room with a light intensity of 2000lx, a photoperiod of 12h / d, and a temperature of 18°C, and the growth conditions were observed regularly.

[0043] The experimental results are shown in Table 5. In terms of inducing the rooting of oyster leaf buds, considering the excessive growth of buds, root length and rooting rate, a better m...

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Abstract

The invention relates to the culture and reproduction capability of mertensia maritime and discloses a tissue culture bud reproduction method for mertensia maritime, which comprises the following steps: sequentially carrying out vibration rinsing on an explant in 75% alcohol, soaking and vibrating in a 0.1% mercury bichloride solution and vibrating and soaking in aseptic water, and then inoculating the explant into an aseptic bud culture medium for culture to obtain mertensia maritime aseptic buds; (2) inoculating a proliferation culture medium with the cultured mertensia maritime aseptic buds in an ultra-clean workbench to obtain subcultured aseptic seedlings; (3) taking out the subcultured aseptic seedlings from the ultra-clean workbench, carrying out plant division treatment, and then respectively inoculating a rooting culture medium with the aseptic seedlings to obtain mertensia maritime seedlings with roots; and (4) taking out the mertensia maritime seedlings with the roots for root washing, transplanting the mertensia maritime seedlings to a cultivation plate for seedling hardening and seedling raising, and carrying out separate planting culture to obtain mertensia maritime products. The high-quality mertensia maritime can be rapidly propagated by the method, and the mertensia maritime varieties and the excellent characteristics can be preserved.

Description

technical field [0001] The invention relates to the technical field of cultivation and propagation of oyster leaves, and discloses a method for tissue culture bud propagation of oyster leaves. Background technique [0002] Oyster leaf (Mertensia maritime), also known as oyster leaf (oyster leaf), is a genus of seagrass in the family Boraginaceae. It is native to temperate to cold regions of the northern hemisphere such as Northern Europe, Norway and Iceland, Northeast Asia such as Russia and Hokkaido, Japan, and other coastal gravel or pebble beaches. A very unique species of alpine perennials on the golden sandy pebble beaches of the sea. It has succulent, glaucous, bluish-green leaves and small, pointed, bell-shaped, pink to blue flowers with a prostrate growth habit, blooming from mid-June to August. Because oyster leaves have an oyster-like flavor, they can be used as a substitute for oysters, and are often used in French salads or appetizers. However, in addition to i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/002
Inventor 龚化勤林河也郑娜娜
Owner FUJIAN SANAN SINO SCI PHOTOBIOTECH CO LTD
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