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Polygonatum sibiricum seedling raising method

A sealwort and seed technology, applied in the field of sealwort seedlings, can solve the problems of long seedling time, low seedling efficiency, high cost, etc., and achieve the effect of increasing germination rate, improving cell viability, and low cost

Inactive Publication Date: 2019-12-20
怀化市九天界生态农业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, most of the existing sealwort adopts slicing breeding, and the cost is high. Most of the existing seed breeding is to select mature and plump seeds for sand storage immediately after the seeds mature in August, and to transplant after 1 year, which also needs to meet the growth requirements of sealwort. Shade conditions, long seedling time, low seedling efficiency

Method used

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  • Polygonatum sibiricum seedling raising method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] see figure 1 , a Polygonatum seedling raising method, is characterized in that, comprises the following steps:

[0031] (1) Seed disinfection: collect mature Polygonatum fruit, soak for 12-24 hours, take out the seeds, soak in ethanol for 30s, then take out, then soak in HgCl, soak in HgCl for 25-30min, wash 1-3 times, and then place Soak in salicylic acid solution for 25 minutes, then wash with distilled water for 3-5 times, place in a sterilized environment, and dry in the air; soak the seeds in 70% ethanol for 30±5 seconds, the treatment pollution rate is 30%, and does not affect the seeds The natural growth of natural growth requires high concentration and time, and it needs to be accurately grasped, otherwise the higher the concentration or the longer the time, the pollution rate will plummet, but it will affect the seedling cultivation and cause the necrosis of the seedlings; soak the seeds with 0.1% HgCl The pollution rate of 25-30min treatment is 10%, and it do...

Embodiment 2

[0034] see figure 1 , a Polygonatum seedling raising method, the steps are as follows:

[0035]a. After soaking the seeds for 12 hours, first soak them in ethanol for 25 seconds, and then soak them in HgCl for 25 minutes; b. Then rinse them with sterile water for 3 times, soak them in salicylic acid solution for 25 minutes, put them in a sterilized environment, and dry them in the air; c. 1. The sterile material obtained is inoculated on the Ms medium under aseptic conditions. After the seeds germinate, they are transferred to 1 / 2Ms medium after 6 days to simulate the living conditions of Rhizoma Polygonatum, control the temperature difference between day and night, and control the light time at 10 hours , to induce callus formation, and then form seedlings in the tissue culture bottle; d, train the seedlings in step c, and add exogenous additives; e, when the seedlings grow to 2-3cm, transplant them, and The roots are sprayed with a microfertilizer solution; the total time r...

Embodiment 3

[0037] see figure 1 , a Polygonatum seedling raising method, the steps are as follows:

[0038] a. After soaking the seeds for 24 hours, first soak them in ethanol for 35 seconds, and then soak them in HgCl for 25 minutes; b. Then rinse them with sterile water for 5 times, soak them in salicylic acid solution for 25 minutes, put them in a sterilized environment, and dry them in the air; c. 1. The aseptic material obtained is inoculated on the Ms medium under aseptic conditions. After the seeds germinate, they are transferred to 1 / 2Ms medium after 8 days to simulate the living conditions of Polygonatum, control the temperature difference between day and night, and control the light time at 10 hours , to induce callus formation, and then form seedlings in the tissue culture bottle; d, train the seedlings in step c, and add exogenous additives; e, when the seedlings grow to 2-3cm, transplant them, and The roots are sprayed with micro-fertilizer solvent; the total time required i...

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Abstract

The invention discloses a polygonatum sibiricum seedling raising method. The method comprises the following steps of seed collection and disinfection, sterile culture, seedling hardening and transplanting. According to the method, by adopting seeds of mature capsules for direct breeding and regulating the temperature, light, and humidity of a culture chamber, the seeds are induced for seedling raising through sterile culture, the cost is low, seedling raising is quick, the hereditary is good, the polygonatum sibiricum seedling raising efficiency is improved; after seedling hardening treatment,the survival rate of seedlings is increased, and the reproduction is vigorous.

Description

technical field [0001] The invention relates to the technical field of methods for cultivating rhizoma rhizomes, in particular to a method for cultivating rhizoma rhizomes. Background technique [0002] Polygonatum, also known as: Chicken head Polygonatum, Yellow Chicken Vegetable, Pen Tube Vegetable, Claw Ginseng, Tiger Ginger, Chicken Claw Ginseng, is a plant of the genus Polygonatum, with horizontal rhizomes, cylindrical, enlarged nodules, whorled leaves, sessile, medicine Plants can nourish the spleen, moisten the lungs and promote body fluids. Choose a moist and well-shaded plot. The soil should be loamy or sandy loam with loose texture and good water retention. Before planting, turn it deep once, and combine it with site preparation every day. Apply 2,000 kg of farmyard manure per mu, turn it into the soil as base fertilizer, and then rake and level it to make a furrow with a width of 1.2 meters. [0003] However, most of the existing sealwort adopts slicing breeding,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G22/25A01C1/00A01C1/08A01G13/00A01H4/00C05G3/00C05G3/02
CPCA01G22/25A01C1/00A01C1/08A01G13/00A01H4/008C05B7/00C05G3/00
Inventor 向建华
Owner 怀化市九天界生态农业科技有限公司
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