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Device and method for fast synthesizing ectotrophic mycorrhiza

An ectomycorrhizal and rapid technology, applied in the fields of botany equipment and methods, horticulture, plant cultivation, etc., can solve the problems of cumbersome methods and long time, and achieve the effect of increasing the infection rate, reducing the dosage and reducing the cost.

Pending Publication Date: 2019-10-08
NANJING JINPU LANDSCAPE
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Problems solved by technology

This method increases the infection rate of ectomycorrhizal fungi by increasing the number of lateral roots of seedlings, but this method is relatively cumbersome and takes a long time, requiring more than 3 months

Method used

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  • Device and method for fast synthesizing ectotrophic mycorrhiza

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Embodiment 1

[0028] A device for rapidly synthesizing ectomycorrhizae, comprising a seedling-growing room, the outside of the seedling-growing room 1 is a square petri dish, and two small openings of 2 cm × 1 cm are opened on one side thereof; Stretching the bacterial agent carrying film 2, on which the ectomycorrhizal fungal inoculum 3 is attached; the mycorrhizal fungal inoculum 3 is in contact with the root system of the seedling stock 4.

[0029] Use device among the present invention to cultivate ectomycorrhizal black pine, comprises the steps:

[0030] 1) Cultivate black pine seedlings: buy new seeds collected in the autumn of the year, soak and disinfect with 10% (volume ratio) hydrogen peroxide for 30 minutes. Soak in deionized water for 30 minutes, and place in a dark incubator at 35°C for 24 hours to accelerate germination. The seeds were sown in a square sieve (40 cm×25 cm×10 cm) filled with sterilized perlite, and an impermeable turnover box (40 cm×25 cm×15 cm) was placed unde...

Embodiment 2

[0039] Effect of different strains on infection rate.

[0040] Using the device of the present invention to synthesize ectomycorrhizae, the infection rates of different bacterial strains were compared, see Table 2 for details.

[0041] Table 2

[0042] strain type normal method The device of the present invention colored bean puffball 55% 72% Amethyst wax mushroom 59% 84% Delicious Porcini Mushrooms 48% 69% Rhizopus flavonoides 43% 57%

[0043] It can be seen that the use of the mycorrhizal synthesis device of the present invention to cultivate mycorrhizal seedlings is superior to conventional fungus through the infection tests of four different bacteria such as colored bean puffball, amethyst wax mushroom, delicious boletus, and rhizophyllum. The method of mixing soil with blocks, and the time is shortened, and the cost is relatively reduced.

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Abstract

The invention belongs to the technical field of seedling breeding and discloses a device for fast synthesizing ectotrophic mycorrhiza. The device comprises a seedling culture chamber of a square culture dish structure and is characterized in that one side of the square culture dish is provided with a small opening, and a microbial agent bearing film bestrewed with ectotrophic mycorrhiza fungus mycelia is placed inside the seedling culture chamber. By the device, mycorrhizal plants with high mycorrhization rate can be synthesized fast, mycorrhiza synthesizing time is shortened greatly, the useamount of a seedling culture medium is reduced, and cost is lowered.

Description

technical field [0001] The invention belongs to the technical field of nursery stock breeding, in particular to a device and method for rapidly synthesizing ectomycorrhizae. Background technique [0002] Ectomycorrhizal seedlings refer to the addition of ectomycorrhizal agents to the seedling substrate when cultivating seedlings, so that ectomycorrhizal fungi can infect the roots of seedlings and form ectomycorrhizal symbionts. The seedlings successfully infected by the fungus are ectomycorrhizal seedlings. The symbiosis of ectomycorrhizal fungi with seedlings can promote the utilization of water and nutrients by seedlings, improve the adaptability and stress resistance of seedlings, and the survival rate of seedlings will also increase. Therefore, this technology has great application prospects in seedling planting. Big. But there is certain defective in the method for synthesizing ectomycorrhiza seedlings at present, as synthetic ectomycorrhizae time is too long, general...

Claims

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Application Information

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IPC IPC(8): A01G18/10A01G31/00A01G31/02
CPCA01G18/10A01G31/00A01G31/02Y02P60/21
Inventor 汤阳泽刘雁丽庄凯顾亚兰沈秋菊王春浩
Owner NANJING JINPU LANDSCAPE
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