33 results about "Ectomycorrhiza" patented technology

Disclosed is a method of preparing Tricholoma matsutake-infected young pine trees by coculturing aseptic seedlings of a pine tree with a T. matsutake isolate, comprising the steps of liquid-culturing fruit bodies of naturally occurring matsutake mushrooms in PDB (Potatto Dextrose Broth, Difco) and inoculating T. matsutake KBFERI 20T05 isolated from the cultured fruit bodies; placing mixed soil of perlite and sphagnum peatmoss and K-liquid medium onto the inoculated fungus; and planting aseptic seedlings obtained by aseptically germinating pine seeds into the mixed soil, and then culturing the pine seedlings with the T. matsutake isolate to allow ectomycorrhiza formation on the fine roots of the pine seedlings. The method of the present invention is advantageous in terms of giving objectivity for the fungal inoculum by employing as an inoculum a fungus having DNA homology with the conventionally known T. matsutake, and of making it possible to mass produce T. matsutake-infected pine trees throughout the Year.

The invention discloses a method for optimizing nursery-grown plant cultivation by using ectomycorrhizal fungi. The method comprises the main process including the following steps: production of an ectomycorrhizal fungi agent, cultivation of tree seedlings, inoculation with the ectomycorrhizal fungi, and nutrition management of the tree seedlings after inoculation. In actual use, a pre-experiment can be performed according to different tree species, and a best ectomycorrhizal fungi strain is selected. 6 kinds of ectomycorrhizal fungi are adopted as materials to inoculate root systems of the tree seedlings, and the growth amount and the transplanting survival rate of the tree seedlings are significantly improved.

The invention relates to a method for forming mycorrhizae after two types of ectotrophic mycorrhiza fungi, namely sparassis crispa and tricholoma matsutake infect seedlings of several kinds of pinus plants and belongs to the fields of artificial ectotrophic mycorrhiza fungus infection seedling-growing application technology and agricultural cultivation technology. The method comprises the steps of 1, acquisition and cultivation of sterile seeds of the pinus plants; 2, selection and treatment of a culture substrate; 3, selection and treatment of seedling-growing hole trays; 4, preparation before infection treatment; 5, infection treatment of the sparassis crispa and the tricholoma matsutake; 6, seedling stage management after infection treatment. The seedlings with the mycorrhizae successfully formed through infection grow stronger than the seedlings not subjected to infection, the leaf color is thick green, euphylla grows out in advance, disease resistance is enhanced, the symbiotic relationship between the sparassis crispa, the tricholoma matsutake and the several kinds of pinus plants under controllable conditions is creatively and successfully created, a study case can be provided for the symbiotic relationship between plants and fungi in the study of the ectotrophic mycorrhiza fungi, and a solid scientific foundation is laid for realizing artificial cultivation of the ectotrophic mycorrhiza fungi.

The invention relates to the field of biology, in particular to a method for promoting the formation of ectomycorrhizae by plants, comprising the following steps: (1) preparing bacterial liquid: breaking the bacterial strain and soaking it in an enzyme liquid capable of decomposing the cell wall of the rhizosphere cell of the host plant (2) infecting the host: soaking the rhizosphere of the host plant in the bacterial solution and then planting, and the bacterial strain and the host have an ectomycorrhizal symbiotic relationship. The invention can significantly increase the quantity of ectomycorrhizae formed after the bacterial strain infects the host.

The invention discloses a brevibacillusreuszeri which is categorized and named as brevibacillusreuszeri MPt17. The brevibacillusreuszeri is collected in China Center for Type Culture Collection on December 1st, 2011 with a collection number of CCTCC NO:M2011433. The brevibacillusreuszeri MPt17 is advantaged in that: the brevibacillusreuszeri MPt17 can substantially promote the growth of ectomycorrhiza fungus such as pisolithustinctorius and xerocamuschrysenteron. Also, as a result of greenhouse pot culture experiments, the double-inoculated mycorrhiza auxiliary bacterium MPt17 and pisolithustinctorius or xerocamuschrysenteron can substantially promote the growth and development of pinus massoniana seedlings. Therefore, the brevibacillusreuszeri MPt17 provided by the invention assists in providing excellent strain resources for the development of pinus massoniana growth-promoting microbial manure, and has a good application and development prospect.

The invention provides an ectomycorrhizal fungus with Cr(VI) tolerance and reducing ability, which is classified as Pisolithus sp. and has a preservation number of CCTCC No: M2018087. The application of hexavalent chromium in contaminated soil, and the application of plant-microbe joint remediation of chromium-contaminated soil. The invention also provides a method for using the fungus to jointly restore chromium-contaminated soil by plants and microbes. The ectomycorrhizal fungus Pisolithus sp. obtained by the separation and screening of the present invention is resistant to Cr(VI) and has the ability to remove it, which is of great significance to the removal of hexavalent chromium in chromium-contaminated soil. On this basis, a plant-microbial joint The new remediation system for remediation can give full play to the advantages of both, and greatly improve the remediation efficiency of heavy metal Cr(VI) contaminated soil.

The invention provides russula vinosa Lindbl yield increasing fungi and a using method thereof, which belong to the technical field of russula vinosa Lindbl yield increase and solve the problems of low yield, high price, low quality and the like of wild russula vinosa Lindbl of the prior art. The russula vinosa Lindbl yield increasing fungi of the invention are a mixed flora of locally common ectomycorrhizal fungi and comprise Boletus brunneissimus Chiu, R. albida Peck, Boletus luridus and amanita yellow corner. Mycelia of various fungi of the russula vinosa Lindbl yield increasing fungi are cultured by a shaker culture method respectively; the mycelia are separate and used to prepare mycelium mixtures at a concentration of 50mg / L respectively; the mixtures are mixed uniformed in the sameamount before inoculation to form the russula vinosa Lindbl yield increasing fungi; and on a woodland wherein russula vinosa Lindbl grows, the russula vinosa Lindbl yield increasing fungi are inoculated in dent pits by a root cutting and mycorrhization technique. In the method, ectomycorrhizal fungi flora is used to increase the biomass of thin roots of arbor, so the fruiting capacity, yield and quality of wild russula vinosa Lindbl are improved obviously and remarkable economic benefits are created. Thus, the method is suitable to be promoted and used in large areas.

The invention discloses a mycorrhiza helper bacterium Bacillus spp and its application in populus growth promotion. The above mycorrhiza helper bacterium is preserved in the China Center for Type Culture Collection in Wuhan University in Wuhan, China on April 6, 2013 as Bacillus spp. MY-DZ18, and has a preservation number of CCTCC NO:M2013125. The Bacillus spp. MY-DZ18 is a mycorrhiza helper bacterium, can promote the mycelia growth of two ectomycorrhizal fungi comprising Pisolithus tinctorius and Lactarius insulsus; pot experiment results show that the respective double inoculation of the MY-DZ18 strain with the two ectomycorrhizal fungi substantially improves the mycorrhiza formation rate of the root of the populus, and promotes the growth of the populus; and the MY-DZ18 strain has an antagonistic effect on Botryosphaeria dothidea Phomopsis sp., Cytospora chrysosperma and seedling damping-off pathogen Fusarium oxysporum.

The invention provides a multi-mycorrhizal inoculation method for woody plants, which overcomes the limitation of a single strain and achieves the effect that the same woody plant can be infected by multiple ectomycorrhizal fungi, which is better than single strain inoculation Promote plant growth, colonization and improve plant stress resistance. At the same time, this method can be applied to the vegetation restoration of abandoned land, and multi-inoculated seedlings can better promote the diversity of microbial communities in abandoned land, which has a positive effect on the improvement of abandoned land. After the waste land is improved by inoculating more seedlings, it is conducive to the improvement of biodiversity, and has a positive effect on land fertility, soil physical and chemical properties and other indicators. The inoculation of the present invention uses the successfully infected mother seedling mycorrhizae to inoculate the seedlings, and the pollution rate is greatly reduced. At the same time, the soil matrix is ​​used to cultivate the plants to be inoculated, and the plants grow vigorously.

The invention discloses a preparation of ectomycorrhizal fungus granules, which comprises the following steps: (1) stirring and smashing the mycelium of ectomycorrhizal fungi to obtain ectomycorrhizal fungus liquid; Add the liquid into the ectomycorrhizal liquid of step (1) to obtain a mixed liquid; (3) slowly add the mixed liquid in step (2) into polyacrylamide, continue stirring until the mixed liquid is completely absorbed, and then add wheat bran powder and continue stirring to obtain the ectomycorrhizal fungus granule strain; the sowing method adopts root cutting on an inclined plane or root cutting in a hole shape. The ectomycorrhizal fungus granules strains of the present application have improved the problems of the prior art, which are limited by the distribution of the target mycorrhizal fungi, and the labor intensity of sowing is high, and the problems of being greatly affected by the weather, and the raw materials used are simple and easy to obtain, the preparation method is simple, and the operation is easy. The method is simple and convenient, reduces the production cost, improves the economic benefit, and is suitable for popularization and application in a large area.