33 results about "Ectomycorrhiza" patented technology

The invention discloses a method for optimizing nursery-grown plant cultivation by using ectomycorrhizal fungi. The method comprises the main process including the following steps: production of an ectomycorrhizal fungi agent, cultivation of tree seedlings, inoculation with the ectomycorrhizal fungi, and nutrition management of the tree seedlings after inoculation. In actual use, a pre-experiment can be performed according to different tree species, and a best ectomycorrhizal fungi strain is selected. 6 kinds of ectomycorrhizal fungi are adopted as materials to inoculate root systems of the tree seedlings, and the growth amount and the transplanting survival rate of the tree seedlings are significantly improved.

The invention provides russula vinosa Lindbl yield increasing fungi and a using method thereof, which belong to the technical field of russula vinosa Lindbl yield increase and solve the problems of low yield, high price, low quality and the like of wild russula vinosa Lindbl of the prior art. The russula vinosa Lindbl yield increasing fungi of the invention are a mixed flora of locally common ectomycorrhizal fungi and comprise Boletus brunneissimus Chiu, R. albida Peck, Boletus luridus and amanita yellow corner. Mycelia of various fungi of the russula vinosa Lindbl yield increasing fungi are cultured by a shaker culture method respectively; the mycelia are separate and used to prepare mycelium mixtures at a concentration of 50mg/L respectively; the mixtures are mixed uniformed in the same amount before inoculation to form the russula vinosa Lindbl yield increasing fungi; and on a woodland wherein russula vinosa Lindbl grows, the russula vinosa Lindbl yield increasing fungi are inoculated in dent pits by a root cutting and mycorrhization technique. In the method, ectomycorrhizal fungi flora is used to increase the biomass of thin roots of arbor, so the fruiting capacity, yield and quality of wild russula vinosa Lindbl are improved obviously and remarkable economic benefits are created. Thus, the method is suitable to be promoted and used in large areas.

The invention discloses a brevibacillusreuszeri which is categorized and named as brevibacillusreuszeri MPt17. The brevibacillusreuszeri is collected in China Center for Type Culture Collection on December 1st, 2011 with a collection number of CCTCC NO:M2011433. The brevibacillusreuszeri MPt17 is advantaged in that: the brevibacillusreuszeri MPt17 can substantially promote the growth of ectomycorrhiza fungus such as pisolithustinctorius and xerocamuschrysenteron. Also, as a result of greenhouse pot culture experiments, the double-inoculated mycorrhiza auxiliary bacterium MPt17 and pisolithustinctorius or xerocamuschrysenteron can substantially promote the growth and development of pinus massoniana seedlings. Therefore, the brevibacillusreuszeri MPt17 provided by the invention assists in providing excellent strain resources for the development of pinus massoniana growth-promoting microbial manure, and has a good application and development prospect.

The present invention relates to a method for colonizing a root-organ from a plant with a mycorrhizal fungus that is representative of the naturally process of colonization of plant roots by mycorrhizal fungi. The present invention also relates to the use of this method for studying in vitro the colonization of plant roots with a mycorrhizal fungus and to an in vitro model for studying the colonization of plant roots with a mycorrhizal fungus.

The invention provides an efficient and practical castanea mollissima blume ectomycorrhiza soil pot inoculation system and an application thereof, and relates to the technical field of ectomycorrhizaculture. The inoculation system comprises a culture soil pot, a matrix, a solid culture medium with fungal hyphae and castanea mollissima blume seedlings, wherein the matrix is sterilized vermiculite;the castanea mollissima blume seedlings are planted in the culture soil pot with the vermiculite as the matrix; the solid culture medium with the fungal hyphae is placed around the root systems of the castanea mollissima blume seedlings for a circle and is placed at the position 3 cm away from the soil surface as much as possible; and the fungal hyphae on the solid culture medium make contact with the root systems of the castanea mollissima blume seedlings. By means of the inoculation system, the infection rate of the root systems of the castanea mollissima blume seedlings and fungi can be effectively increased, the generation time of ectomycorrhizae is shortened, the formation efficiency of the ectomycorrhizae of the castanea mollissima blume can be improved to 100% in the culture soil pot; and mycorrhizae of different plants are consistent.

Disclosed is a method of preparing Tricholoma matsutake-infected young pine trees by coculturing aseptic seedlings of a pine tree with a T. matsutake isolate, comprising the steps of liquid-culturing fruit bodies of naturally occurring matsutake mushrooms in PDB (Potatto Dextrose Broth, Difco) and inoculating T. matsutake KBFERI 20T05 isolated from the cultured fruit bodies; placing mixed soil of perlite and sphagnum peatmoss and K-liquid medium onto the inoculated fungus; and planting aseptic seedlings obtained by aseptically germinating pine seeds into the mixed soil, and then culturing the pine seedlings with the T. matsutake isolate to allow ectomycorrhiza formation on the fine roots of the pine seedlings. The method of the present invention is advantageous in terms of giving objectivity for the fungal inoculum by employing as an inoculum a fungus having DNA homology with the conventionally known T. matsutake, and of making it possible to mass produce T. matsutake-infected pine trees throughout the Year.

The invention discloses an anti-blocking zero-valent iron coupling response type slow-release persulfate two-stage permeable reactive barrier device and application thereof, and belongs to the technical field of groundwater pollution remediation. According to the technical scheme, two stages of reactive barriers are arranged at the downstream of a pollution plume; a first-stage reaction region anda second-stage reaction region are respectively formed in the two stages of reactive barriers; the first-stage reaction region is positioned at the upstream of the second-stage reaction region; the first-stage reaction region is filled with a mixed filler; the secondary reaction region is filled with a slow-release filler; perennial deep-rooted plants are planted at the top end of the first-stagereaction region; and iron-proof ectotrophic mycorrhiza is inoculated around the roots of the perennial deep-rooted plants. The two reaction regions cooperate with each other to degrade the chloro-hydrocarbon organic pollutants in groundwater, the groundwater is efficiently repaired, secondary pollution is prevented, and the device has the advantages of being more economical, efficient, ecologicaland environmentally friendly.

The invention provides a seedling mycorrhization method with a mycorrhizal fungus mycelium culture medium as a seedling culture medium and application of the seedling mycorrhization method, and belongs to the technical field of seedling mycorrhization. The method specifically comprises the following steps: inoculating ectomycorrhizal fungus liquid into a pretreated mycelium culture medium for culturing, carrying out crushing and uniform mixing, and then filling a seedling culture hole tray with the mixture for continuously culturing, so as to obtain a seedling culture medium containing active mycelia; and putting pretreated seeds into the seedling culture medium containing the active mycelia for culturing. The mycorrhization method provided by the invention is simpler, quicker and more efficient, and has important significance in popularization of application of mycorrhizal fungi and realization of mycorrhizal industrialized production of improved varieties of forest and flowers.

The invention discloses preparation of an ectomycorrhizal fungus granule strain. The preparation comprises the following steps: (1) stirring and smashing ectomycorrhizal fungi to obtain mycelium, so as to obtain ectomycorrhizal fungus liquid; (2) adding a nutrient solution into the ectomycorrhizal fungus liquid obtained in the step (1), so as to obtain a mixed solution; and (3) slowly adding the mixed solution obtained in the step (2) into polyacrylamide, continuing to stir until the mixed solution is completely adsorbed, then adding wheat bran powder, and continuing to stir, so as to obtain the ectomycorrhizal fungus granule strain. A sowing method of the ectomycorrhizal fungus granule strain is carried out by adopting inclined-plane root breaking or cave-shaped root breaking. The ectomycorrhizal fungus granule strain disclosed by the invention has the advantages that the problems that the prior art is limited by distribution of target mycorrhizal fungi, the labor intensity of sowingis high and the influence of weather is larger are improved; and the used raw materials are simple and easy to obtain, the preparation method is simple, the operation is simple and convenient, the production cost is reduced, the economic benefit is increased, and the preparation is suitable for large-area popularization and application.

The invention provides a forming material with storage and capacity expansion functions and a preparation method and application thereof, and belongs to the technical field of preparation of microorganisms and water-retaining materials. The forming material with the storage and capacity expansion functions is obtained by inoculating ectomycorrhizal fungi to a high-water-absorption culture medium and culturing the ectomycorrhizal fungi. According to the invention, the mycorrhizal fungus mycelium is used as a binder, various organic water-absorbing materials are formed, the forming material with the storage and capacity expansion functions is prepared, a new technical scheme is provided for fully utilizing water resources and improving the water utilization efficiency, and the forming material has good practical application value.

The invention discloses a pinus massoniana ectomycorrhizal strain coating agent which comprises 5-30 parts of ectomycorrhizal liquid and 5-30 parts of a water-retaining agent, 5-20 parts of an adhesive; 4-8 parts of humus soil; 20 to 50 parts of a growth regulator, and 0.005 to 0.03 part of a growth regulator; and 30-55 parts of water. The invention discloses a preparation method of a pinus massoniana ectomycorrhizal strain coating. The preparation method comprises the following steps: uniformly mixing hypha liquid containing boletus mucilaginosus, colored puffball and lactarius deliciosus, humus soil, gibberellin and an adhesive, slowly adding purified water, continuously stirring, stopping adding water when the mixture is stirred to be sticky, and standing the mixture until the mixture is in a solidified state. The use method comprises the following steps: seed disinfection: preparing 2L of 600-fold liquid from 75% chlorothalonil wettable powder with good adhesion, broad-spectrum sterilization and low toxicity, soaking seeds for 1 hour, and washing the seeds with distilled water for 3 times for later use; seed coating: coating the disinfected seeds for later use and the mixed seed coating agent in a seed coating machine, wherein the mass ratio of the seed coating agent to the seeds is 2: 1. The method has the characteristics of improving seed germination rate, promoting seedling growth and improving seedling resistance.