18 results about "Ectomycorrhiza" patented technology

The invention provides a slope ecological greening construction method based on soil improvement and a monitoring system, and is suitable for the technical field of slope greening construction. The method comprises the steps of plant-fungus combination selection, slope protection structure construction, hypha system construction, unmanned aerial vehicle sowing, earth surface film mulching and dynamic maintenance regulation and control. A mycorrhizal network is constructed by taking arbuscular mycorrhizal fungi and ectomycorrhizal fungi as cores, a mycelium fiber network is formed, and the soil fixation capability is enhanced by the cohesive force between the fiber network and soil and the strength of fibers; the stability of soil aggregates is improved through glomalin secreted by hyphae, and the corrosion resistance of the soil is further improved; a water-retaining agent is added, so that the water-retaining property of the backfill soil is improved while early-stage growth of green vegetation and a hypha network is guaranteed; finally, the unmanned aerial vehicle carries a multispectral camera and a radar for cooperative scanning, rapidly and accurately monitors the soil moisture content, sets a soil moisture content warning value, reminds maintenance personnel to irrigate and maintains long-term survival of vegetation.

The invention relates to methods of cultivating ectomycorrhizal fungi, the method comprising incubating ectomycorrhizal MAT1-1 mycelia, ectomycorrhizal MAT1-2 mycelia, and ectomycorrhizal male mycelia in a culture medium for about 1 day to about 50 days, thereby cultivating ectomycorrhizal fungi to produce an ectomycorrhizal fungal biomass comprising MAT1-1 ectomycorrhizal mycelia, MAT1-2 ectomycorrhizal mycelia, and male ectomycorrhizal mycelia. Also provided are methods of use of the ectomycorrhizal mycelial biomass.

This invention discloses a cultivation experimental device for studying plant ectomycorrhizal fungal interactions. It includes a base with a petri dish on it. A protective shell is installed around the petri dish on the top of the base. A circular plate is rotatably mounted inside the protective shell. Both the circular plate and the top surface of the protective shell have two symmetrical arc-shaped ventilation openings. An adjustment component connects the circular plate and the protective shell, including a splined sleeve rotatably connected to the top of the protective shell. This invention provides a cultivation experimental device for studying plant ectomycorrhizal fungal interactions that offers high adjustment precision, effortless operation, and stability. The precise design of the ventilation opening adjustment component allows for accurate control of the opening size, meeting the oxygen requirements of different fungal species and plants. Furthermore, the threaded connection of the protective shell enhances the stability and practicality of the device, providing strong support for scientific research.

The present application belongs to the technical field of truffle planting, and particularly relates to a truffle planting formula and a planting method for shortening the initial yield period of truffles. In view of the problems of poor growth and development of mycorrhizal roots, poor mycorrhizal root vitality, small number of mycorrhizal roots, high contamination rate of ectomycorrhizal miscellaneous bacteria, long initial yield period of truffles, and low yield, the present application provides a truffle planting formula and a planting method. The formula can effectively promote the growth of truffle seedlings, the development of truffle mycorrhizal roots, reduce the contamination rate of ectomycorrhizal miscellaneous bacteria, accelerate the formation of fire rings, shorten the initial yield period of truffles by 2-5 years, and improve the yield of truffle planting.

The invention discloses a method for culturing ectomycorrhiza of brilliant lactarius deliciosus, which comprises the following steps: S1, culturing brilliant lactarius deliciosus on a culture medium A to obtain a brilliant lactarius deliciosus colony; s2, disinfecting the seeds of the pinus massoniana; s3, the disinfected seeds are inoculated to a culture medium B to be cultured until the seeds germinate to form masson pine seedlings, after the masson pine seedlings grow lateral roots, the masson pine seedlings are moved to a culture medium C, the brilliant lactarius deliciosus colonies are inoculated to the edge of the roots, and ectomycorrhiza of the brilliant lactarius deliciosus on the masson pine root system is obtained after culture. Through the construction of a sterile in-vitro culture system, four defects of a traditional soil culture method are systematically solved, the research on a brilliant lactarius deliciosus-masson pine symbiosis mechanism can be accelerated, and efficient technical support is provided for mycorrhizal seedling production, forest ecological restoration and artificial domestication of edible mushrooms.

The invention provides a carbon sink type tailing soil conditioner as well as a preparation method and a soil treatment method thereof. The invention relates to a carbon sink type tailing soil conditioner, which comprises porous biochar loaded with an alkali-producing microbial agent and a multifunctional composite microbial agent with heavy metal toxicity reduction and efficient carbon sequestration effects; the alcaligenes microbial agent comprises alcaligenes; the multifunctional complex microbial inoculant comprises heavy metal passivating bacteria, carbonic anhydrase secreting bacteria and mycorrhizal fungi; the mycorrhizal fungi comprise at least one of arbuscular mycorrhizal fungi and ectotrophic mycorrhizal fungi; the porous biochar is prepared from the following raw materials: sodium carbonate and organic waste powder. According to the method, the rapid weathering of the tailings, the reconstruction of the soil structure and the efficient storage of CO2 are realized, the soil period is shortened from more than 5 years to 8-12 months, the carbon sink strength is improved to 2.1-3.4 t CO2 / ha.a, and an innovative solution is provided for the goals of ecological restoration and carbon neutralization of mines.

The invention discloses an application of an ectomycorrhizal fungus inoculant in promoting growth of loblolly pine. The invention finds that ectomycorrhizal fungi lactarius deliciosus fungi can promote the growth of loblolly pine, by inoculating the lactarius deliciosus fungi on loblolly pine at a specific month age and regulating and controlling the lactarius deliciosus ectomycorrhizal fungi, the growth environment and nutritional status of the loblolly pine are improved, and the absorption of the loblolly pine on nutrients is promoted, so that the growth of the loblolly pine is effectively promoted; the mycorrhiza infection rate of the loblolly pine can be obviously improved, and the growth amount and the chlorophyll content of the loblolly pine can be obviously increased, so that a remarkable growth promoting effect on the loblolly pine is achieved.

The utility model relates to a visual ectomycorrhizal seedling raising cup, and belongs to the field of seedling raising cups. Comprising an inner cup layer made of a transparent material, an anti-scattering ring and an outer cup layer made of a non-transparent material, the anti-scattering ring is of an annular structure, the anti-scattering ring is slidably arranged on the outer wall of the inner cup layer in a sleeving mode in the axial direction of the inner cup layer, the inner cup layer is sleeved with the outer cup layer during seedling raising, and the bottom end of the anti-scattering ring abuts against the top end of the outer cup layer; the inner cup layer comprises a plurality of inner cup layer cup walls and an inner cup layer base, the inner cup layer cup walls are wound to form an annular structure, and the bottom ends of the inner cup layer cup walls are arranged in the circumferential direction of the inner cup layer base and hinged to the circumferential side edge of the inner cup layer base. According to the utility model, the root system of the sample plant is protected from being influenced by the external environment, convenient conditions are provided for scientific research personnel to intuitively observe the growth condition of the root system and sample, and the container or foundation soil and even the sample plant are prevented from being damaged.

This invention discloses a combined substrate, device, and method for enhancing ectomycorrhizal symbiosis. The combined substrate comprises a fertilizer-attracting substrate, a fertilizer-isolating substrate, and a fungal-attracting substrate. The fertilizer-attracting substrate layer is composed of river sand and vermiculite; the fertilizer-isolating substrate layer is quartz sand; and the fungal-attracting substrate layer is vermiculite. The particle size of both the fertilizer-attracting and fungal-attracting substrate layers is smaller than that of the fertilizer-isolating substrate layer. The volume ratio of the fertilizer-attracting, fertilizer-isolating, and fungal-attracting substrate layers is (5-7):(1-2):(2-3). This invention addresses the lack of strictness in the symbiosis between ectomycorrhizal fungi and host trees. Based on the symbiotic characteristic that ectomycorrhizal fungi primarily assist host trees in absorbing mineral nutrients, it provides a simple, efficient, and highly feasible method that allows fungi to quickly adapt to the actual forest environment in a short period and maintain a long-term, non-degrading symbiotic relationship with the host trees. Later, only routine forest management of the ectomycorrhizae is required.

The invention provides a method for improving the survival rate of a young poplar man-made forest in a sandy and arid region and the soil quality of forest land based on mycorrhizal symbiosis, and belongs to the technical field of drought-resistant afforestation. The water-retaining agent provided by the invention is prepared from the following components in parts by mass: 60 to 80 parts of acrylic acid, 20 to 30 parts of humic acid, 10 to 15 parts of chitosan, 50 to 70 parts of a pH value regulator, 0.1 to 1 part of N, N-methylene bisacrylamide and 0.5 to 5 parts of potassium persulfate. The water-retaining agent prepared by the invention is high in repeated water absorption capacity and strong in fertilizer-retaining capacity, and has wide development prospects in the aspects of agriculture and forestry water retention, soil fertility increasing, soil physicochemical property improvement and the like. The prepared water-retaining agent is mixed with biochar, organic fertilizer, ectomycorrhiza liquid and soil according to a certain proportion, then the mixture is buried in a fertilization ditch, ectomycorrhiza and poplar seedlings are symbiotic, the absorption area of root systems is increased, the soil environment is improved, the survival rate of the poplar seedlings can be effectively increased, and the physicochemical property of the soil is improved.

The invention discloses an application of an ectomycorrhizal fungus inoculant in promoting the growth of slash pines. It is found that ectomycorrhizal fungi lactarius deliciosus fungi can promote growth of slash pines, the lactarius deliciosus fungi are inoculated on the slash pines at the specific month age, the growth environment and nutritional conditions of the slash pines are improved through regulation and control of the ectomycorrhizal fungi of the lactarius deliciosus, nutrient absorption of the slash pines is promoted, and then growth of the slash pines is effectively promoted; the mycorrhiza formation rate of the slash pines can be remarkably improved, and the growth amount and chlorophyll content of the slash pines are remarkably increased, so that a remarkable growth promoting effect on the slash pines is achieved.

This invention relates to a method for promoting ectomycorrhizal fungal infection based on electrically driven earthworms, thereby improving the survival rate of afforestation in difficult sites. The method includes the following steps: (1) selecting mycorrhizal fungi and earthworms that can form a stable symbiotic relationship to construct a mycorrhizal-earthworm coupling system; (2) constructing an electric power system: setting up an electric power system around the tree trunk, installing the mycorrhizal-earthworm coupling system described in step (1) into the electric power system, and energizing it. Compared with existing technologies, this invention can increase the infection rate of ectomycorrhizal fungi at the root tips of trees, promote the growth, health, and survival rate of trees in difficult sites, thereby achieving the purpose of soil carbon sequestration and enhancement.

The invention discloses a cultivation method of a cyclobalanopsis gilgii and phoebe zhejiangensis mixed forest. The cultivation method comprises the following steps: selecting slightly acidic red soil with a gentle slope at the altitude of 0-250m; cyclobalanopsis rubra, phoebe zhejiangensis and other Lauraceae woody plants are planted, the plant row spacing is 1.6 m * 1.6 m, strip-shaped mixed planting is carried out, and the same-age forest is cultivated into a stable and high-quality precious wood tree species mixed forest through periodic tending management. According to the method, the mycorrhizal tree species of cyclobalanopsis gilgiana and the non-mycorrhizal tree species of phoebe chekiangensis are planted in a mixed mode, the abundance of endophytic mycorrhizal fungi in soil is increased by means of the symbiotic relationship of the cyclobalanopsis gilgiangensis and the ectomycorrhizal fungi, the nutrient absorption efficiency of the root system of phoebe chekiangensis is improved, meanwhile, the advantage that the phoebe chekiangensis trees grow fast is utilized, light resources are reasonably configured, and the yield is increased. Growth of lateral branches of cyclobalanopsis gilgii is slowed down, the growth rate of trunks is increased, and the yield and quality of dry wood are improved. The method has the advantages of being accurate in cultivation technology, vigorous in forest tree growth, low in production cost and easy and convenient to operate, and has wide application prospects.

The invention discloses a Guilin acinetobacter A59 and an application thereof. The collection number of the Guilin acinetobacter A59 is CCTCC (China Center For Type Culture Collection) NO: M 20252694. The acinetobacter Guilin A59 which is exogenously added can increase the hypha growth of pure culture lactarius deliciosus fungi, promote the symbiosis of pine-lactarius deliciosus mycorrhiza, namely increase the number of mycorrhizal tips, improve the plant height and biomass of mycorrhizal seedlings, increase the nitrogen, phosphorus, potassium and iron contents of overground parts of the mycorrhizal seedlings and the nitrogen content of root systems, and improve the growth of the pure culture lactarius deliciosus fungi. Therefore, the strain can be applied to promoting synthesis and / or growth of a pinus plant-lactarius deliciosus symbiotic system and increasing the mycorrhizal degree of mycorrhizal seedlings, so that growth and nutrient absorption of the pinus plant mycorrhizal seedlings are promoted, and the quality of ectotrophic mycorrhizal seedlings is improved.

This invention relates to the field of microbial inoculant technology, and discloses *Agrobacterium pusa* F32 with ectomycorrhizal-promoting activity and its applications. *Agrobacterium pusa* F32 is deposited at the Guangdong Provincial Microbial Culture Collection Center, located at 5th Floor, Building 59, No. 100 Xianlie Middle Road, Guangzhou, on January 9, 2026, with accession number GDMCC No:67611, and classified as follows: Agrobacterium pusense In this invention, *Pseudomonas aeruginosa* F32, isolated from the fruiting body of *Lycoperdon perlatum*, an ectomycorrhizal fungus of *Pinus kesiya*, exhibits excellent solubility for insoluble organic and inorganic phosphorus, enhancing the absorption and accumulation of mycorrhizal phosphorus in *Pinus kesiya*. It not only promotes mycorrhizalization of plant roots and increases mycorrhizal infection rate, but also promotes the growth of *Pinus kesiya* under conditions of inoculation alone or co-inoculation with ectomycorrhizal fungi, showing a synergistic effect with ectomycorrhizal fungi. Therefore, it can be used to prepare biological agents that promote the growth of ectomycorrhizal seedlings such as *Pinus kesiya*.

The invention discloses a pinus kesiya young forest ternary biological synergistic soil fertility improving method, which comprises ectomycorrhizal fungus inoculation treatment, local nitrogen fixation leguminous plant intercropping treatment and cutting and returning treatment, mycorrhizal inoculation adopts colored puffball or Yunnan puffball bacterial suspension to be applied to base soil of pinus kesiya, intercropping treatment adopts interplanting of flemingia macrophylla, and interplanting treatment adopts interplanting of flemingia macrophylla. The step of cutting and returning to the field refers to regular cutting, turning and pressing, ternary biological synergy enables the soil organic matter to be increased by 0.3%-0.5% annually, the growth amount of the pinus khasys is increased by 15%-25%, and zero chemical fertilizer input is achieved.

The invention relates to the technical field of masson pine monitoring, and discloses an observation device for monitoring symbiosis of masson pine root systems and ectomycorrhizal fungi in real time, the observation device comprises a light-transmitting outer cylinder and a light-shielding ring fixed at the upper end of the light-transmitting outer cylinder, the inner side of the light-transmitting outer cylinder is provided with a light-transmitting middle cylinder, the inner side of the light-transmitting middle cylinder is provided with a light-transmitting inner cylinder, and the light-transmitting inner cylinder is provided with a light-shielding ring fixed at the upper end of the light-transmitting outer cylinder. And an adjustable monitoring mechanism is mounted at the upper end of the interior of the light-transmitting inner cylinder in a penetrating manner. The shading liquid in the annular water tank is injected into the first cavity to form a shading layer, and light can be effectively prevented from interfering with natural growth of masson pine roots; a second cavity used for storing water and fertilizer is formed between the light-transmitting middle cylinder and the light-transmitting inner cylinder. Temperature and humidity at different heights in soil are collected through the temperature and humidity sensor, pH values at different heights in the soil are collected through the pH value sensor, and then the roots of the pinus massoniana are accurately watered and fertilized through the seepage pipe, so that the growth conditions of the roots of the pinus massoniana under different water and fertilizer conditions can be conveniently observed.

The present application discloses a method for estimating the contribution of ectomycorrhizal fungi to nitrogen acquisition by Abies minjiangensis and Betula sclerosa, which relates to the technical field of estimating the contribution of plants to nitrogen acquisition. The method comprises collecting leaf, stem and root samples of ectomycorrhizal trees in a target area and soil samples directly below the ectomycorrhizal trees; measuring the leaf, stem and root samples to determine the nitrogen stable isotope values ​​and nitrogen contents of the leaf, stem and root samples; measuring the soil sample to determine the nitrogen stable isotope value of the total dissolved nitrogen of the soil sample; and calculating the contribution rate of ectomycorrhizal fungi to nitrogen acquisition by ectomycorrhizal trees using an isotope mass balance model and an isotope mixing effect model based on the nitrogen stable isotope values ​​and nitrogen contents of the leaf, stem and root samples and the nitrogen stable isotope value of the total dissolved nitrogen of the soil sample. The present application can improve the accuracy of estimating the contribution of ectomycorrhizal fungi to nitrogen acquisition by Abies minjiangensis and Betula sclerosa.