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A method for promoting the formation of ectomycorrhizae in plants

A technology of ectomycorrhizae and plants, applied in the biological field, can solve the problems of low breeding rate of artificial cultivation and achieve the effect of increasing the number

Active Publication Date: 2021-11-02
YUNNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide a method for promoting the formation of ectomycorrhizae in plants, so as to solve the problem of low breeding rate of many existing strains of artificial cultivation

Method used

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  • A method for promoting the formation of ectomycorrhizae in plants
  • A method for promoting the formation of ectomycorrhizae in plants
  • A method for promoting the formation of ectomycorrhizae in plants

Examples

Experimental program
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Effect test

Embodiment 1

[0021] A method for promoting plants to form ectomycorrhizae, comprising the steps of:

[0022] (1) Preparation of bacterial liquid: after crushing the dry bacteria strain, soak it in the enzyme liquid that can decompose the cell wall of the rhizosphere cells of pine seedlings to obtain the bacterial liquid, the soaking temperature is 6°C, and the soaking time is 6 hours; the enzyme liquid is fiber Sulfase, water, the concentration of described cellulase is 300U / ml.

[0023] (2) Infecting the host: Soak the rhizosphere of the pine seedlings in the bacterial solution before planting, and replenish 40 ml of the original enzyme solution to the rhizosphere of the pine seedlings every 20 days.

Embodiment 2

[0025] A method for promoting plants to form ectomycorrhizae, comprising the steps of:

[0026] (1) Preparation of bacterial liquid: after crushing the dry bacteria strain, soak it in the enzyme liquid that can decompose the cell wall of the rhizosphere cells of pine seedlings to obtain the bacterial liquid, the soaking temperature is 2°C, and the soaking time is 12 hours; the enzyme liquid is fiber Sulfase, water, the concentration of described cellulase is 300U / ml.

[0027] (2) Infecting the host: Soak the pine seedlings in the bacterial solution before planting, and supplement the rhizosphere of the pine seedlings with 10 ml of the original enzyme solution every 10 days.

Embodiment 3

[0029] A method for promoting plants to form ectomycorrhizae, comprising the steps of:

[0030] (1) Preparation of bacterial liquid: after crushing the dry bacteria strain, soak it in the enzyme liquid that can decompose the cell wall of pine seedling rhizosphere cells to obtain the bacterial liquid, the soaking temperature is 4°C, and the soaking time is 10 hours; the enzyme liquid is lacquer Enzyme, water, the concentration of described laccase is 20U / L.

[0031] (2) Infecting the host: Soak the rhizosphere of the pine seedlings in the bacterial solution before planting, and supplement the rhizosphere of the pine seedlings with 10 ml of the original enzyme solution every 20 days.

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Abstract

The invention relates to the field of biology, in particular to a method for promoting the formation of ectomycorrhizae by plants, comprising the following steps: (1) preparing bacterial liquid: breaking the bacterial strain and soaking it in an enzyme liquid capable of decomposing the cell wall of the rhizosphere cell of the host plant (2) infecting the host: soaking the rhizosphere of the host plant in the bacterial solution and then planting, and the bacterial strain and the host have an ectomycorrhizal symbiotic relationship. The invention can significantly increase the quantity of ectomycorrhizae formed after the bacterial strain infects the host.

Description

technical field [0001] The invention relates to the field of biology, in particular to a method for promoting the formation of ectomycorrhizae in plants. Background technique [0002] In recent years, due to a large number of collections and the deterioration of the growth environment, the number of many large fungi has been decreasing day by day. People's predatory harvesting prevents the fruiting bodies from being retained until the stage of radiospores, and their normal life history is cut off, resulting in an increasing decline in production. obvious. Now, by artificially separating, purifying and screening excellent strains, the artificially cultivated mycelium is infected to the host, so that the mycelium and the host form ectomycorrhizae, and finally grow into fruiting bodies. The first half of this method is manual operation, and the second half is completed under natural conditions, that is, semi-artificial cultivation. Improving the infection rate of mycelium inf...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G18/10
CPCA01G18/10
Inventor 沙涛王丁卉张亚平沙小茗景跃波张林
Owner YUNNAN UNIV
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