The invention relates to the technical field of detection
reagent preparation, and discloses a stable oxidized low-density
lipoprotein cholesterol detection kit and a preparation method thereof.The kit is composed of a component R1 and a component R2, the component R1 at least comprises a
buffer solution, a sensitizer, a surfactant, a stabilizer, an anti-interference agent and a
preservative, and the component R2 at least comprises a
buffer solution, a sensitizer, a surfactant, a stabilizer, an anti-interference agent and a
preservative; the component R2 at least comprises an
ion buffer solution, a stabilizer, a sealing agent, an
antibody-carboxyl
microsphere conjugate and a
preservative; the
antibody-carboxyl
microsphere conjugate is a conjugate of an oxidized low-density
lipoprotein antibody and carboxyl microspheres. According to the formula of the oxidized low-density
lipoprotein reagent composition, the
reagent R1 and the reagent R2, the production cost is effectively reduced, the oxidized low-density lipoprotein reagent composition can be used on a full-automatic biochemical analyzer, the
automation degree is high, compared with similar products, the oxidized low-density lipoprotein reagent composition has higher accuracy,
repeatability and stability, and unexpected technical effects are achieved.