Pine or fir artificial forest stress-tolerant growth-promoting composite mycorrhizal fungi inoculant, and preparation and application methods thereof

An application method and technology of ectomycorrhizal fungi, applied in the directions of botanical equipment and methods, applications, biocides, etc., can solve the problems of difficulty in the production of endomycorrhizal fungi, high production costs, application limitations, etc.

Inactive Publication Date: 2015-05-13
CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, compared with ectomycorrhizal fungal agents, the production of endophytic mycorrhizal (AM) inoculants in China is more difficult, the production cost is high, and its application in actual production has been limited. There is no commercial product for the high-efficiency compound mycorrhizal fertilizer formed by the optimized combination of roots

Method used

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  • Pine or fir artificial forest stress-tolerant growth-promoting composite mycorrhizal fungi inoculant, and preparation and application methods thereof
  • Pine or fir artificial forest stress-tolerant growth-promoting composite mycorrhizal fungi inoculant, and preparation and application methods thereof
  • Pine or fir artificial forest stress-tolerant growth-promoting composite mycorrhizal fungi inoculant, and preparation and application methods thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Example 1: Screening of excellent exogenous strains

[0016] Ectomycorrhizal fungus Seleroderma polyrhizum Pers., Scleroderma cepa Pers., Lactarius hatsudake Tanaka, Lactarius akahatsu, Suillus Suillus luteus, Suillus bovines, Cantharellus cibarius Fr., Russula vesca Fr., Amanita phalloides, Scleroderma bovista Fr.10 seed entities were isolated from the comprehensive PDA by tissue Culture, culture conditions: medium is comprehensive PDA (20% potato juice 1000mL, glucose 18g, potassium dihydrogen phosphate 3.0g, magnesium sulfate 1.2g, agar 20g, vitamin B10.01g); culture temperature range is 25°C-28°C ; The pH range is 5-6; The culture medium is prepared for 7 days of first-class plate culture. Observe the growth of each strain.

[0017] After the first-class species were obtained, the back grafting experiment was used to detect the effect on the mycorrhizal synthesis ability of Pinus massoniana and Pine slash seedlings. 4 months after inoculation, from the results of...

Embodiment 2

[0022] Example 2: Excellent combination screening of endogenous and exogenous bacterial strains

[0023] The endophytic mycorrhizal fungi are Glomus mosseae or Glomus intraradices; the ectomycorrhizal fungi are multi-rooted hard puffballs and red milk mushrooms;

[0024] Among them: the endophytic mycorrhizal agent is a mixture containing infectious spores, mycorrhizae and culture substrate obtained by the multiplication and cultivation of the greenhouse pot culture method. Specifically, Glomus mosseae or Glomus intraradices use sorghum as the host plant, sand: vermiculite: soil = 2:1:1 as the culture substrate, and 20 plants / 5L pots as the cultivation density. At the same time, 30% Hoagland's nutrient solution was applied twice a week. After 3-4 months of culture, the spore densities in the culture medium can reach 103 / ml and 97 / ml respectively, and the mycorrhizal infection rates can reach 89.6% and 88.9% respectively.

[0025] The ectomycorrhizal agent is a secondary liquid...

Embodiment 3

[0033] Embodiment 3: Screening of different ratios of each bacterial strain in the composite mycorrhizal agent

[0034] Mix the endogenous and exogenous mycorrhizal agents in different proportions on the ultra-clean workbench, carry out pot experiments on pine fir seedlings, and comprehensively compare the inoculation results of different proportions. The appropriate ratio range of Russula russulae is 1-3:1-2:1; among them, the A2 treatment group has the best effect on the biomass and mycorrhizal infection rate of pine fir seedlings, that is, Glomus moesei:Scleroderma multiroot Pimabo:Red milk mushroom=3:2:1; followed by A1 treatment group. The results are shown in Table 3. (Unless otherwise specified, the ratio of bacterial strains occurring in the present invention all refers to the mass ratio of the culture after cultivation, such as: Glomus mosseae or Glomus intraradices are obtained by the expansion and cultivation of the greenhouse pot culture method Containing a mixtu...

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Abstract

The invention discloses a stress-tolerant growth-promoting composite mycorrhizal fungi inoculant for a pine or fir artificial forest, and a preparation and application methods thereof. The composite mycorrhizal fungi inoculant is compounded from an endomycorrhizal fungi inoculant and an exotrophic mycorrhiza fungi inoculant, wherein the endomycorrhizal fungi inoculant is obtained by culturing Glomus mosseae or Glomus intraradices in pots in a greenhouse; and the exotrophic mycorrhiza fungi inoculant is obtained by fermenting Lactarius hatsudake Tanaka and Scleroderma polyrhizum Pers. in a liquid fermentation medium. In the forestation process, the seedling root system is subjected to slurry dipping treatment or embedded in pine or fir forest soil, thereby promoting rootage and growth of pines or cedars, improving the tree vigor, enhancing the stress tolerance, increasing the absorptivity of P, and preventing the pine leaf cast, fir anthracnose, pine seedling blight and cataplexis.

Description

Technical field: [0001] The present invention relates to a pine and fir plantation anti-stress and growth-promoting compound mycorrhizal agent for promoting masson pine, slash pine and fir rooting and growth, improving water and nutrient metabolism, improving soil, anti-stress health care and improving productivity, and its preparation and application The application method has a good inhibitory effect on pine and fir plantation diseases, and improves the absorption and utilization efficiency of P. Background technique: [0002] my country's planted forest area accounts for nearly one-third of the world's total planted forest area, ranking first in the world. However, there are many major problems in my country's plantation forests, such as the increasing damage of diseases and insect pests, the serious decline of soil fertility, and the decline of productivity, which seriously threaten the construction of plantation forests and the sustainable management of forests. In ord...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N63/04A01P21/00A01G1/00
Inventor 周国英李琳刘君昂黄馨董文统杨菁刘成锋刘倩丽田媛媛
Owner CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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