Seedling mycorrhization method with mycorrhizal fungus mycelium culture medium as seedling culture medium and application of seedling mycorrhization method

A technology of mycelium culture and seedling substrate, applied in the fields of application, botany equipment and methods, cultivation, etc., can solve the problems of slow growth of mycorrhizal fungi, prolong the time of mycorrhizalization, restrict growth and reproduction, etc., and achieve the improvement of mycorrhizal The effect of increasing the intensity of mycorrhizalization, shortening the time of mycorrhizalization, and improving the survival rate of transplanting

Inactive Publication Date: 2021-08-06
SHANDONG FOREST SCI RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, mycorrhizal fungi grow slowly, are difficult to achieve pure culture, and have the characteristics of high symbiosis with plants and obligate nutrition. In addition, mycorrhizalization of seedlings is affected by various factors (fungal characteristics, host characteristics, and environmental factors, etc.), The new inoculation carrier (seedling-raising substrate) and seedling-raising environment have largely restricted the growth and reproduction of mycorrhizal fungus efficient propagules (mycelium), making the growth rate of mycelium closely related to that of seeds, cutting branches, transplanted seedlings or broken plants. The root growth rate of the root seedlings does not match, which reduces the contact area that

Method used

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  • Seedling mycorrhization method with mycorrhizal fungus mycelium culture medium as seedling culture medium and application of seedling mycorrhization method
  • Seedling mycorrhization method with mycorrhizal fungus mycelium culture medium as seedling culture medium and application of seedling mycorrhization method
  • Seedling mycorrhization method with mycorrhizal fungus mycelium culture medium as seedling culture medium and application of seedling mycorrhization method

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preparation example Construction

[0031] Wherein, the filler includes a mixture of peat, perlite and vermiculite. In a specific embodiment of the present invention, the preparation method of the filler comprises: in parts by mass, mixing 45-55 parts of peat, 20-30 parts of perlite and 20-30 parts of vermiculite, adding water to adjust The water content reaches 55-65% and mixed evenly to obtain the above-mentioned filler.

[0032] The second aspect of the present invention provides the application of the above seedling mycorrhization method in seedling cultivation. Through the above-mentioned mycorrhizal treatment, the survival rate of seeds can be effectively improved, and the growth of seeds and seedlings can be promoted.

Embodiment 1

[0034] Embodiment 1: the application of the mycorrhized seedling raising method that takes the mycelium growth substrate as the seedling raising substrate

[0035] In this example, the ectomycorrhizal fungus Xerocomus badius was inoculated with different formula substrates, and the growth of the fungus was observed, and the ryegrass was sown as a seedling substrate, and the germination of the seeds and the growth of the seedlings were observed. X.badius was purchased from the China Forestry Microorganism Collection and Management Center, and the preservation number is cfcc5946. The specific experimental steps are as follows:

[0036] 1. Inoculate the X.badius parent species on PDA solid medium (pH=6.5), carry out plate activation and proliferation culture, and culture under constant temperature and humidity (25±1°C, 60.00%) for 1 week under dark conditions. Use a sterile puncher with a diameter of 5mm to punch holes on the edge of the activated colony, pick the mycelium block...

Embodiment 2

[0046] Embodiment 2: the application of the mycorrhized seedling raising method that is the seedling raising substrate with mycelium growth substrate

[0047] In this example, ryegrass was taken as an example to compare the colonization of the ectomycorrhizal fungus Xerocomus badius in the root system of ryegrass under different inoculation methods. X.badius was purchased from the China Forestry Microorganism Collection and Management Center, and the preservation number is cfcc5946. The specific experimental steps are as follows:

[0048] 1. Inoculate the X.badius parent species on PDA solid medium (pH=6.5), carry out plate activation and proliferation culture, and culture under constant temperature and humidity (25±1°C, 60.00%) for 1 week under dark conditions. Use a sterile puncher with a diameter of 5mm to punch holes on the edge of the activated colony, pick the mycelium block and place it in the PDB liquid medium, and place it in a constant temperature and humidity rotar...

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Abstract

The invention provides a seedling mycorrhization method with a mycorrhizal fungus mycelium culture medium as a seedling culture medium and application of the seedling mycorrhization method, and belongs to the technical field of seedling mycorrhization. The method specifically comprises the following steps: inoculating ectomycorrhizal fungus liquid into a pretreated mycelium culture medium for culturing, carrying out crushing and uniform mixing, and then filling a seedling culture hole tray with the mixture for continuously culturing, so as to obtain a seedling culture medium containing active mycelia; and putting pretreated seeds into the seedling culture medium containing the active mycelia for culturing. The mycorrhization method provided by the invention is simpler, quicker and more efficient, and has important significance in popularization of application of mycorrhizal fungi and realization of mycorrhizal industrialized production of improved varieties of forest and flowers.

Description

technical field [0001] The invention belongs to the technical field of mycorrhizalization of seedlings, and in particular relates to a method for mycorrhizalization of seedlings using mycorrhizal fungus mycelium culture substrate as a seedling raising substrate and an application thereof. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] Mycorrhiza is a common plant symbiosis phenomenon in nature. It is a composite absorption organ formed by soil beneficial fungi (mycorrhizal fungi) and roots of higher plants, and is a mutualistic symbiosis. More than 85% of terrestrial plant species can establish mutualistic symbiotic relationships with various types of mycorrhizal fungi t...

Claims

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Application Information

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IPC IPC(8): A01G18/20A01G18/50
CPCA01G18/20A01G18/50
Inventor 刘丙花马海林刘方春刘幸红杜振宇马丙尧
Owner SHANDONG FOREST SCI RES INST
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