Seedling mycorrhization method with mycorrhizal fungus mycelium culture medium as seedling culture medium and application of seedling mycorrhization method

Abstract

The invention provides a seedling mycorrhization method with a mycorrhizal fungus mycelium culture medium as a seedling culture medium and application of the seedling mycorrhization method, and belongs to the technical field of seedling mycorrhization. The method specifically comprises the following steps: inoculating ectomycorrhizal fungus liquid into a pretreated mycelium culture medium for culturing, carrying out crushing and uniform mixing, and then filling a seedling culture hole tray with the mixture for continuously culturing, so as to obtain a seedling culture medium containing active mycelia; and putting pretreated seeds into the seedling culture medium containing the active mycelia for culturing. The mycorrhization method provided by the invention is simpler, quicker and more efficient, and has important significance in popularization of application of mycorrhizal fungi and realization of mycorrhizal industrialized production of improved varieties of forest and flowers.

Description

technical field [0001] The invention belongs to the technical field of mycorrhizalization of seedlings, and in particular relates to a method for mycorrhizalization of seedlings using mycorrhizal fungus mycelium culture substrate as a seedling raising substrate and an application thereof. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] Mycorrhiza is a common plant symbiosis phenomenon in nature. It is a composite absorption organ formed by soil beneficial fungi (mycorrhizal fungi) and roots of higher plants, and is a mutualistic symbiosis. More than 85% of terrestrial plant species can establish mutualistic symbiotic relationships with various types of mycorrhizal fungi t...

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Problems solved by technology

However, mycorrhizal fungi grow slowly, are difficult to achieve pure culture, and have the characteristics of high symbiosis with plants and obligate nutrition. In addition, mycorrhizalization of seedlings is affected by various factors (fungal characteristics, host characteristics, and environmental factors, etc.), The new inoculation carrier (seedling-raising substrate) and seedling-raising environment have largely restricted the growth and reproduction of mycorrhizal fungus efficient propagules (mycelium), making the growth rate of mycelium closely related to that of seeds, cutting branches, transplanted seedlings or broken plants. The root growth rate of the root seedlings does not match, which reduces the contact area that can be infected, prolongs the mycorrhization time, affects the mycorrhization effect, and even leads to the failure of mycorrhization

The rhizotomy mycorrhizal seedling cultivation method slows down the growth rate of primary roots through root cutting, so that the fungal invasion mode is mainly based on cross-sectional wounds, expanding the colonization area, making colonization and infection occur at the same time, improving the colonization intensity, and achieving However, this method needs to pull out the seedlings, cut the roots, return to the bed to mycorrhizate, and transplant. The process is cumbersome, time-consuming and labor-intensive, and it is easy to cause mechanical damage to the seedlings, and the cost is too high

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