54 results about "Mutualistic symbiosis" patented technology

The invention relates to a microorganism bacterium agent for treating industrial wastewater pollution. The microorganism bacterium agent is prepared from pseudomonas putida, candida utilis, bacillus amyloliquefaciens, clostridium papyrosolvens, nitrosomonas europaea, paracoccus denitrificans and auxiliary materials. The microorganism bacterium agent has the advantages that various bacteria in mutualistic symbiosis are compatible reasonably without antagonism and are effective in degradation of phenyl amines degradation-resistant compounds when being thrown into a wastewater treatment system, thereby being suitable for industrial wastewater treatment and being capable of increasing treatment water quantity, improving treatment water quality, reducing operational cost and promoting up-to-standard discharge.

The invention discloses a preparation of compound rice dedicated bio-fertilizer based on arbuscular mycorrhizal fungi and an application thereof, and relates to the preparation and the application of bio-fertilizer. The compound rice dedicated bio-fertilizer aims to solve the problem that rice can not be infected by the arbuscular mycorrhizal fungi under the aquatic condition at present and is difficult to effectively form mutualistic symbiosis, and therefore the rice can not obtain mineral nutrition from an arbuscular mycorrhizal fungi-crop symbiotic system. The method comprises the following steps of: preparing a colonized reinforcer; pre-processing arbuscular mycorrhizal fungi fungicide; and evenly mixing the processed arbuscular mycorrhizal fungi fungicide, rice shoots growth promoterand the colonized reinforce. The compound rice dedicated bio-fertilizer can be prepared into a seedling bed, culturing the mycorrhiza seedling and managing a rice field. According to the compound rice dedicated bio-fertilizer disclosed by the invention, the arbuscular mycorrhizal fungi-crop symbiotic system with a high infection rate and stable performance can be built in the seedling stage of the rice, and the symbiotic system can successfully live through the transplanted field flooding environment. The biological bactericide activity loss problem brought by flooding intimidation can be effectively solved, mineral nutrition is continuously provided for the rice, the growth situation of the rice is improved, the fertilizer application amount in the field is reduced, and the rice production cost can be effectively lowered.

The invention discloses a biological straw decomposition agent and a preparation method thereof and particularly relates to a biological straw decomposition agent taking funguses and bacteria as raw materials and a production method of the biological straw decomposition agent. According to the biological straw decomposition agent, trichoderma longibrachiatum, aspergillus terreus, phanerochaete chrysosporium, paenibacillus amylolyticus and torulopsis candida are taken as compound stains; a cellulolytic enzyme, a semi-cellulolytic enzyme and a ligninolytic enzyme are mainly produced; the strains have good mutualistic symbiosis and metabiosis effects and have no antagonisms. When the biological straw decomposition agent is used, the decomposition agent is uniformly scattered into fields after crop straws (wheat, corns and rice) are crushed and dispersed on the ground; the dosage of the biological straw decomposition agent is 30kg per 1hm<2> and then the straws and the decomposition agent are ploughed under the ground.

The invention relates to an establishing method of symbiont of cyclobalanopsis glaucoides and bolete, belonging to the field of biotechnology (plant tissues and fungal culture). In the research of mycorrhizal fungi, a stable pure symbiont of plants and fungus is difficult to establish. At present, bolete can not be cultivated artificially easily, belongs to mycorrhizal fungi capable of realizing mutualistic symbiosis with the plant, and has symbiotic relationship with pinaceae plants, fagaceae plants and the like under natural condition. According to the establishing method, cyclobalanopsis glaucoides seed and generalized delicious beef liver mushroom entity collected in the Wuding lion rock are used as materials, so that callus and mycelium are respectively induced successfully and are cultured together in an innovation manner, and a simple and stable pure-symbiont system is established under the sterile state. Important basis is provided for the symbiotic relationship and the symbiotic mechanism of bolete, cyclobalanopsis glaucoides and the other plants and the molecular mechanism of the beef liver mushroom entity and the like, and the method use for reference is provided for research on other macro fungi and symbiotic plants.

The invention provides microorganism compound microbial inoculums capable of efficiently degrading straw lignin. Floras achieve mutualistic symbiosis and synergistic interaction. In a straw fermentation process, three enzymes relating to the degradation of the lignin can be secreted at the same time; the activities of laccase, lignin peroxidase and manganese peroxidase are respectively 23.56 U/g,23.02 U/g and 21.74 U/g biomass; the lignin content of the fermented corn straws is reduced by 60.21 percent, and loss of sugar such as celluloses and hemicelluloses is avoided; the conversion rate ofthe celluloses in the pretreated corn straws is greater than that of non-treated corn straws by 142.14 percent; a screened compound bacterial strain is high in lignin degradation capacity, and the industrial utilization efficiency of the straws pretreated by the bacterial strain is obviously improved. The research provides a new pretreatment way for enzymolysis saccharification of lignocellulosesin the straws.

The invention provides a method for producing a functional biological organic base fertilizer by using chicken manure. The method is characterized by taking the raw materials such as pig manure, chicken manure and humus as the base, adjusting a best carbon-nitrogen ratio, adopting a deep tank continuous high-temperature aerobic dynamic fermentation process, adding rotten bacteria inoculants capable of facilitating decomposition and rotting of materials and deodorizing into the rotting process, and adding functional bacteria after the materials are rotten to produce high-quality functional biological organic fertilizer. The method has the advantages that an efficient decomposition technology and aerobic fermentation technology system is built, the treatment time of manure of livestock and poultry is shortened, and the production efficiency is improved; the functional bacteria are not mutually antagonized with mutualistic symbiosis; all the microbes can grow and propagate in different temperature zones and mutually exert effects, so that the fermentation effect is ensured fundamentally; the odor of the manure can be effectively removed; the manure of the livestock and poultry can be quickly rotted in 7-10 days.

The invention discloses a grapery intercropping grass chicken raising ecologic planting and breeding technology. The technology comprises the steps of 1 land selection and grapery construction, 2 grape planting, 3 grass planting, 4 baby chick breeding and 5 environment control. According to the grapery intercropping grass chicken raising ecologic planting and breeding technology, the space resources of the grapery are sufficiently utilized, grass is intercropped, high-quality chickens are cultivated three-dimensionally, mutualistic symbiosis and mutual benefits of grapes, grass and chickens are formed, and the technology is a typical fruit-grass-herd planting and breeding combination ecologic operation mode. According to the grapery intercropping grass chicken raising ecologic planting andbreeding technology, the feed consumption is lowered, the chicken external packaging character and quality are improved, the market selling price is increased, and cultivation benefits are greatly improved.

The invention discloses a novel energy conservation and environment protection process for realizing the mutualistic symbiosis of a straw pulping and papermaking industry and a waste lead-acid cell recycling industry. According to the alkaline characteristic of the straw pulping sewage, the acidic characteristic of the waste lead-acid cell recycling sewage, and the condition of the coupling utilization space of the after heat, the energy conservation and environment protection process for realizing the mutualistic symbiosis for comprehensive coupling processing the acid-alkali wastewater and complementarily using the heat energy is provided. By adopting the process, the pollution abatement cost and the difficulty of the two industries can be greatly reduced, the energy complementary utilization is realized and the mutualistic symbiosis of two industries is obtaind. By adopting the process, the complementary utilization of the energy sources of two distant related industries is realized, the alkaline black liquid generated by the straw pulping industry and the leaded acidic wastewater generated by the regenerated lead industry can be completely recycled and utilized, so that the problem of high processing cost of the sewages in two industries can be solved and the economic benefits of two industries can be effectively increased.

A method for breeding lobsterlings in a mountainous area rice field comprises the following steps that S1, a rice field is selected; S2, the rice field is improved; S3, grass is planted with a fertilizer and water, wherein channels are disinfected before water injection and are injected with water after one week; S4, shrimp larvae are put; S5, breeding and management are performed; S6, fishing is performed. The method has the advantages that specific high-quality water resources in a mountainous area and mountain spring water micro-flow hot in winter and cold in summer are utilized to breed the lobsterlings in the rice field, a lobsterling growth environment is reasonably controlled, breeding water quality management is simple, and the lobsterling growth speed is improved, so that bred lobsterlings are bright in color and luster, delicious and high in nutrition value, meanwhile the quality and conditions of the lobsterlings are ensured, and the lobsterlings have a high market value. Lobsterling breeding and rice planting are organically combined, so that lobsterlings and rice produce mutualistic symbiosis, the productivity of farmland is fully played, the additional value of farmland management is improved, and the method has good economic, beneficial and ecological benefits.

The invention relates to a high-efficient low-cost intercropping and interplanting method of Chinese wolfberry and eucommia ulmoides at arid desert regions. The method comprises the following steps of (1) excavating Chinese wolfberry planting trenches; (2) excavating intercropped eucommia ulmoides planting trenches; (3) selecting seedlings: selecting annual juvenile Chinese wolfberry seedlings and annual juvenile eucommia ulmoides seedlings, which are free from diseases and insect pests; (4) performing planting: after soil is unfrozen in the next spring, planting the juvenile Chinese wolfberry seedlings and the juvenile eucommia ulmoides seedlings through a juvenile seedling planter in the manner that as soon as the juvenile Chinese wolfberry seedlings and the juvenile eucommia ulmoides seedlings are pulled up, the pulled-up juvenile Chinese wolfberry seedlings and the pulled-up juvenile eucommia ulmoides seedlings are planted; and (5) taking field management measures. According to the intercropping and interplanting method disclosed by the invention, the Chinese wolfberry and the eucommia ulmoides are intercropped and interplanted, so that the mutualistic symbiosis of the Chinese wolfberry and the eucommia ulmoides under arid desert conditions can be realized, the problems that when only the Chinese wolfberry is planted, the benefits are single and the survival rate of plants is low are solved, the organic unity of ecological benefits, economic benefits and social benefits of desert governance is achieved, and a technical support is provided for safe and high-efficient production of agriculture at the arid desert regions.

The invention relates to a construction method for planting rice and breeding crab, fish and goose in an inland arid region. The method comprises the following steps: selecting suitable rice and river crab varieties; selecting a rice field fit for breeding; digging an annular furrow and setting anti-escape facilities; cultivating rice; stocking juvenile crabs; managing the rice field cultivation; managing the river crab breeding; putting in chub; and stocking Sichuan white gooses. According to the method, the mutualistic symbiosis of rice, river crab and chub is supplied in a rice growth season; after the rice is harvested, the green organic cyclic part of mutualistic symbiosis of rice field and Sichuan white gooses can reduce the cost of planting and breeding in the rice field, can increase the additional value of the rice field and has the characteristics of capabilities of increasing the additional value of the rice field and fully utilizing the rich water resources in the rice planting area in the inland arid region, large planting area of the rice field, less per capita rice field and low per capita income; the manpower and water resources are fully utilized; the requirement of urban people for enjoying green organic food can be met; the cost of planting rice and breeding crab, fish and goose in the rice field can be lowered; the additional value of the rice field is increased; the income of local villager is increased; the local economy is developed.

The invention discloses a method for reducing cadmium accumulation amount and increasing yield of honeysuckle through glomus mosseae. According to the method, glomus mosseae and honeysuckle are cultivated in a mutualistic symbiosis mode. Specifically, firstly, after expanding propagation cultivation is conducted on the glomus mosseae through a host plant, a mixture containing host plant roots, hyphae, mycorrhiza fungal spores and cultivation soil, namely a glomus mosseae agent is obtained; secondly, a culture medium is inoculated with the glomus mosseae agent for honeysuckle cultivation. By means of the method, honeysuckle growing can be promoted, and the yield is increased; more importantly, the cadmium concentration of the overground part (stems, leaves and flowers) and underground part (roots) of the honeysuckle can be reduced. An approach is provided for safely producing the honeysuckle in cadmium polluted soil, the method has great significance in production and application of the honeysuckle, and the method is good in effect, free of secondary pollution and low in cost. Meanwhile, a new thought and a new direction are provided for development and utilization of glomus mosseae.

The invention provides a novel method for stably producing a steinernema carpocapsae F3 generation based on the principle of mutualistic symbiosis of xenorhabdus and nematode and through combination of non-in-vitro culture and in-vitro culture. Healthy galleria mellonella final instar larvae are selected and inoculated with steinernema carpocapsae, and the obtained nematode is the Fo generation; a nutrient agar culture dish with xenorhabdus cultured for two days is inoculated with the nematode Fo generation, and the F1 generation is obtained; finally, an amplification sponge culture medium is inoculated with the F1 generation, culture is carried out for 14 days, and a large quantity of F3 generation is obtained to serve as commercial nematode. The commercial nematode produced through the method is the F3-generation nematode, the production period is short, and the quality is stable.

The invention relates to a mutual assistance symbiosis minimally-invasive inarching method for wild alpine rhododendron ancient stumps and modern cultivars. The method includes the following steps that A, the root systems of the wild alpine rhododendron ancient stumps are processed, and soil around the root systems of ancient trees is scarified and planed, and the roots are exposed; B, the seedlings of the modern cultivars are inarched on the peripheries of the ancient stumps in an encircling mode, and the root systems of inarched plants are made to make close contact with the root systems of the ancient trees as much as possible; C, after the inarching is completed, filling is performed by using the original soil or prepared growth matrix; D, the plants are watered thoroughly for the first time, the root bulbs are fully wetted and repaired. With the mutual assistance symbiosis minimally-invasive inarching method for wild alpine rhododendron ancient stumps and modern cultivars, the inarched plants have a mutual assistance symbiosis relationship, all the organs such as roots, stems and leaves of independent plants are reserved respectively, but the plants depend on one another and are symbiotic in a mutual assistance mode to form a whole.

The present invention relates to a kind of intercropping method suitable for Haloxylon and licorice in arid desert area, the method comprises the following steps: (1) Excavation of Haloxylon planting ditch; (2) Land arrangement of intercropping Glycyrrhiza; Haloxylon seedlings; (4) planting: after the soil thaws in the spring of the second year, use a seedling planter to pick up and plant the Haloxylon seedlings; (5) licorice seed treatment; (6) licorice seed direct seeding: on the cultivation surface between the furrows where Haloxylon is planted Sow the treated licorice seeds on the ground, flatten and suppress with a rake after sowing, and keep moisture; (7) Field management measures. The invention interplants Haloxylon and Glycyrrhiza interplanting, which can realize mutualistic symbiosis of Haloxylon and Glycyrrhiza under arid desert conditions, not only solves the problems of only planting Haloxylon and low plant survival rate, but also achieves ecological, economical and ecological benefits of desert control. The organic unity of social benefits provides technical support for safe and efficient agricultural output in arid desert areas.

The invention discloses a drip irrigation method for planting tree-planted dendrobium officinale. According to the drip irrigation method, during the planting of the tree-planted dendrobium officinale, a water storage device of a drip irrigation device is hung on a planting tree trunk. After that, a flow guide capillary tube is led out of the water storage device. The planting water in the water storage device is continuously led out to the tree foundation at the planting position of the dendrobium officinale by connecting a constant-flow needle with the flow guide capillary tube. According tothe method, the drip irrigation method is adopted. During the planting of the tree-planted dendrobium officinale, the root system of the dendrobium officinale is induced to be effectively combined with the tree body (effective parasitism) through keeping the tree bark to be moist. The survival rate and the yield of the dendrobium officinale are improved. In addition, due to the adoption of the drip irrigation method, the tree body does not need to be damaged during the planting of the tree-planted dendrobium officinale. The health of the planting tree body is not threatened at all. The mutualistic symbiosis of the tree-planted dendrobium officinale and the economic forest is realized. The economic value of the forest land in the unit planting area is effectively improved.

The invention provides a preparation method and an application method for a tea tree in-regulating growth promoting agent. By virtue of the biological characteristics of endogenous herbaspirillum seropedicae of the tea trees, the endogenous herbaspirillum seropedicae is fermented to prepare a tea tree endogenous herbaspirillum seropedicae culture solution and is sprayed to trimmed tea tree branches after being diluted in a certain proportion. Bacteria in the growth promoting agent enter into the tea tree branches for mutualistic symbiosis and secretion and metabolism of active substances so as to promote rapid growth of tea tree sprouts. The treated tea tree sprouts grow vigorously, the tea leaves are dark green, and the length and weight of the sprouts are obviously increased. The technical method provided by the invention is low in cost, simple to operate, green and safe, multiple in function and durable in effect.

The invention discloses a planting method and planting greenhouse for dendrobium officinale. The method comprises the following steps: preparation of a greenhouse, preparation of a cultivation bed, preparation of a substrate, planting, and pest prevention and control. According to the method disclosed by the invention, the overhead cultivation bed and an insect-proof device located on the cultivation bed are built under a certain facility environment, so that the pest prevention and control of the dendrobium officinale is effectively improved; the dendrobium officinale is planted by adopting the method, an incidence rate of pests and diseases of the dendrobium officinale are significantly reduced, and products obtained by the planting method are natural and pollution-free, and have higherquality; and fish can be bred in the process of planting the dendrobium officinale, a novel biological chain is formed, ''fish and dendrobium officinale mutualistic symbiosis'' is achieved, and new artistic conception is added.

The invention discloses a method and device enabling isotope <13>C-<15>N marked endophytic fungi and host plants to be in mutualistic symbiosis, relates to a method and device for researching plant-microorganism symbiotic relationship, and belongs to a method for quantitatively analyzing the effects of microorganisms for promoting hosts to absorb mineral elements through isotope marking tests. Anintegrated device assembled by nylon nets of different hole diameters and organic glass is used as a tool for marking isotope <13>C-<15>N to quantitatively analyze the mutualistic symbiosis relationship of the endophytic fungi and the host plants. The method sufficiently uses the characteristics of the integrated device being quick, accurate and high in controllability, simultaneous marking of various isotopes is performed in the same tree body, and interaction of the endophytic fungi and the host plants is quickly revealed. The integrated marking device for researching the mutualistic symbiosis relationship between the endophytic fungi and the host plants is realized, the problem of quantitatively analyzing the function characteristics of the endophytic fungi is solved, and the isotope marking efficiency and the marking precision are greatly improved.

The invention discloses an environment-friendly enzyme starter and a preparation method thereof. The preparation method includes: screening and separating three kinds of strains of bacteria, fungus and actinomycetes from plant rhizosphere microorganisms; performing spreading cultivation of the strains, liquid state fermentation, centrifugal concentration and spray drying to obtain three kinds of solid bacteria powder, activating the solid strain powder prior to inoculating the same to a mixture medium for spreading cultivation, and after the three bacterial colonies are mutually nutrient and stable in state and subjected to spreading cultivation of the bacterial colonies, adding a protective agent prior to freeze drying to obtain the environment-friendly enzyme starter. The environment-friendly enzyme starter comprises, by weight, 50-70 parts of the solid bacterial powder, 10-20 parts of the solid fungus powder, 5-15 parts of the solid actinomycetes powder, 3-10 parts of the protective agent and 5-10 parts of a fermentation promoter. Mutualistic symbiosis of the different strains is broken through, the microbial colonies are mutually nutrient and stable in state due to composition of the different microbial strains, different microorganisms have different efficacies in environment-friendly enzyme, and function of the various microorganisms can be perfected.

The invention discloses a multi-effect sewage treatment agent and a preparation method thereof. The multi-effect sewage treatment agent is prepared from dry typha latifolia, dry iris wilsonii, dry scirpus tabernaemontani, dry hylocereus undatus, dry India canna, aloes, honey, oligosaccharides, dry yeast and water. The preparation method includes steps of grinding, decocting, extracting, separating, purifying, fermenting and the like. Raw materials of the multi-effect sewage treatment agent are easy to acquire, and the preparation method is simple. The multi-effect sewage treatment agent is capable of activating protozoa, microorganisms and aquatic plants, with a water purifying function, in water to quickly decompose organic matters in sewage under mutualistic symbiosis and synergistic actions, and consequently water purification is realized.

The invention relates to an intercropping and interplanting method of caragana microphylla and lonicera japonica at arid desert regions. The method comprises the following steps of (1) excavating caragana microphylla planting trenches; (2) excavating intercropped lonicera japonica planting trenches; (3) selecting seedlings: selecting annual-biennial juvenile caragana microphylla seedlings and annual juvenile lonicera japonica seedlings, which are free from diseases and insect pests; (4) performing planting: after soil is unfrozen in the next spring, planting the juvenile caragana microphylla seedlings and the juvenile lonicera japonica seedlings through a juvenile seedling planter in the manner that as soon as the juvenile caragana microphylla seedlings and the juvenile lonicera japonica seedlings are pulled up, the pulled-up juvenile caragana microphylla seedlings and the pulled-up juvenile lonicera japonica seedlings are planted; and (5) taking field management measures. According to the intercropping and interplanting method disclosed by the invention, the caragana microphylla and the lonicera japonica are intercropped and interplanted, so that the mutualistic symbiosis of the caragana microphylla and the lonicera japonica under arid desert conditions can be realized, the problems that when only the caragana microphylla is planted, the benefits are single and the survival rate of plants is low are solved, the organic unity of ecological benefits, economic benefits and social benefits of desert governance is achieved, and a technical support is provided for safe and high-efficient production of agriculture at the arid desert regions.