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Method and device enabling isotope C-N marked endophytic fungi and host plants to be in mutualistic symbiosis

A technology of endophytic fungi and mutualism, applied in the field of scientific research on plant-microbe symbiosis, can solve problems such as restricting mutualism, destroying the positive role of beneficial microbial communities, and unreasonable soil microbial community structure.

Pending Publication Date: 2020-10-20
INST OF FORESTRY CHINESE ACAD OF FORESTRY
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Problems solved by technology

[0002] Mineral nutrients are the basis for plant growth and development. However, in traditional agriculture, growers excessively pursue yield, and unreasonable large-scale application of chemical fertilizers changes the structure of soil microbial communities and destroys the positive effect of beneficial microbial communities in the natural environment on host plants. Limits the study of mutualistic symbiotic relationships between root microbial communities and host plants

Method used

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  • Method and device enabling isotope C-N marked endophytic fungi and host plants to be in mutualistic symbiosis

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Embodiment Construction

[0057] The present invention is described below through specific embodiment and accompanying drawing, but the present invention is not limited thereto, any modification, equivalent replacement and improvement etc. that all do within the spirit and principle of the present invention, all should be included in the present invention within the scope of protection.

[0058] The experimental methods used in the following examples are conventional methods unless otherwise specified.

[0059] The materials and reagents used in the following examples can be obtained from commercial sources unless otherwise specified.

[0060] Phialocephala fortinii strain is an endophytic fungal resource of walnut root system isolated from walnut root system in the laboratory and preserved for a long time. The pCT74 plasmid has GFP gene, hygromycin resistance gene and ampicillin resistance gene. pCT74 plasmid: Wuhan Miaoling Biotechnology Co., Ltd.

[0061] Add 200ml PDB culture fluid in 500ml Erle...

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Abstract

The invention discloses a method and device enabling isotope <13>C-<15>N marked endophytic fungi and host plants to be in mutualistic symbiosis, relates to a method and device for researching plant-microorganism symbiotic relationship, and belongs to a method for quantitatively analyzing the effects of microorganisms for promoting hosts to absorb mineral elements through isotope marking tests. Anintegrated device assembled by nylon nets of different hole diameters and organic glass is used as a tool for marking isotope <13>C-<15>N to quantitatively analyze the mutualistic symbiosis relationship of the endophytic fungi and the host plants. The method sufficiently uses the characteristics of the integrated device being quick, accurate and high in controllability, simultaneous marking of various isotopes is performed in the same tree body, and interaction of the endophytic fungi and the host plants is quickly revealed. The integrated marking device for researching the mutualistic symbiosis relationship between the endophytic fungi and the host plants is realized, the problem of quantitatively analyzing the function characteristics of the endophytic fungi is solved, and the isotope marking efficiency and the marking precision are greatly improved.

Description

technical field [0001] The invention belongs to the technical field of scientific research on plant-microbial symbiosis, and specifically relates to a method for studying the mutualism between endophytic fungi and host plants, and also relates to a device for isotope-labeled mutualism between endophytic fungi and host plants. It is suitable for the transportation and distribution of isotope-labeled plant photosynthetic products and mineral elements absorbed by roots. Background technique [0002] Mineral nutrients are the basis for plant growth and development. However, in traditional agriculture, growers excessively pursue yield, and unreasonable large-scale application of chemical fertilizers changes the structure of soil microbial communities and destroys the positive effect of beneficial microbial communities in the natural environment on host plants. This limits the study of mutualistic symbiotic relationships between root microbial communities and host plants. Therefo...

Claims

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Application Information

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IPC IPC(8): A01G18/00A01G18/60A01G18/69A01G17/00A01G9/24A01G9/18A01G9/14A01G7/06G01N27/62C05G1/00C05G5/20
CPCA01G18/60A01G9/14A01G18/00A01G17/005A01G9/245A01G7/06A01G9/18A01G18/69G01N27/62C05B7/00C05G5/20C05C1/00C05D1/02C05D3/00C05D5/00C05D9/00C05D9/02Y02A40/25
Inventor 白永超裴冬张俊佩马庆国
Owner INST OF FORESTRY CHINESE ACAD OF FORESTRY
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