Microorganism compound microbial inoculums capable of efficiently degrading straw lignin

A technology of straw lignin and compound bacterial agent, which is applied in the direction of microorganisms, microorganisms, and methods based on microorganisms, can solve the problems of low lignin degradation rate, high cost, and low activity, and achieve strong lignin degradation ability and industrial utilization The effect of improving efficiency

Active Publication Date: 2018-11-06
JILIN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Physical and chemical methods can reduce the wood content by about 50%, but these two methods are costly and cause secondary pollution
Microbial methods have become a research hotspot due to their simple operation, low cost and g

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Example 1 Mutagenesis screening of Mycobacterium verrucosus

[0017] (1) Morphological identification and screening

[0018] The soil in the forest area of ​​Changbai Mountain Nature Reserve was collected and cultured on PDA medium by conventional methods. The mycelium of the strain was white flocculent at first, and grew divergently towards the periphery. After 8 days of culture, the color of conidia continued to deepen, and glue dots appeared; after 10 days of culture, the colonies were in the shape of concentric rings, and the conidia showed black glue dots. Light brown radial folds appear on the back.

[0019] Use a puncher to get a 1cm-diameter bacterial block, inoculate it on an aniline blue selective medium, and culture it in an aerobic incubator at 30°C for 10 days, observe the fading of aniline blue, and select a strain with a fast fading rate and strong ability as candidate strains.

[0020] At the same time, in order to directional screen lignin-degrading ...

Embodiment 2

[0026] The preparation of embodiment 2 composite bacterial agents

[0027] A microbial composite bacterial agent for efficiently degrading straw lignin. The active ingredient of the bacterial agent is the following raw materials in mass percentage: 40% of laccase-producing Myrothecium verrucosus, 30% of lignin peroxidase-producing Bacillus colioids , Manganese peroxidase-producing Actinomycetes 30%, among the above bacterial agents:

[0028] The lignin peroxidase-producing colloid bacillus (Bacillus mucilaginosus Krassilnikov) GIM1.16;

[0029] The manganese peroxidase-producing Actinomyces Laceyella (Laceyella tengchongensis) CCTCC AA 208050Laceyella; the strains described in the present invention can be purchased from China Center for Type Culture Collection (CCTCC) and Guangdong Microbiology Collection Center (GIM) get.

[0030] The laccase-producing Myrothecium verrucaria T2901 (Myrothecium verrucaria) mutant strain T2901 has a preservation number of CCTCC NO: M2017413; ...

Embodiment 3

[0033] Example 3 Degradation of straw

[0034] The degradation effect of strains on common corn stalks was studied by the method of solid fermentation in a triangular flask. Each 300mL Erlenmeyer flask

[0035] 5g of straws that have been dried and shredded to a width of 0.5 cm and a length of 1-2 cm, (NH 4 ) 2 SO 4 0.2g, MgSO 4 ·7H 2 O0.05g, add 15mL of phosphate buffer solution of 5mM pH 7.0 to each bottle. Each bottle was inoculated with 0.25mL 2.0×10 8 A / mL concentration of the compound bacterial agent suspension, and at the same time take the same amount of sterile water in the control group, mix it and place it at a constant temperature of 20 ℃ for incubation. Regularly observe the morphological changes of the tobacco stalks, and wash them off after 7 days to measure the lignin degradation rate of the corn stalks.

[0036] Drawing of lignin standard curve: prepare 10g / L lignin solution, scan and select the measurement wavelength. Lignin solutions with concent...

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Abstract

The invention provides microorganism compound microbial inoculums capable of efficiently degrading straw lignin. Floras achieve mutualistic symbiosis and synergistic interaction. In a straw fermentation process, three enzymes relating to the degradation of the lignin can be secreted at the same time; the activities of laccase, lignin peroxidase and manganese peroxidase are respectively 23.56 U/g,23.02 U/g and 21.74 U/g biomass; the lignin content of the fermented corn straws is reduced by 60.21 percent, and loss of sugar such as celluloses and hemicelluloses is avoided; the conversion rate ofthe celluloses in the pretreated corn straws is greater than that of non-treated corn straws by 142.14 percent; a screened compound bacterial strain is high in lignin degradation capacity, and the industrial utilization efficiency of the straws pretreated by the bacterial strain is obviously improved. The research provides a new pretreatment way for enzymolysis saccharification of lignocellulosesin the straws.

Description

technical field [0001] The invention relates to a microbial compound bacterial agent for efficiently degrading straw lignin, which belongs to the intensive agricultural production technology, and is specially used for degrading waste corn straws to produce organic fertilizers and realize resource utilization of organic wastes. Background technique [0002] The shortage of chemical energy and the increasingly serious environmental pollution make people constantly seek renewable green energy. As a carbon-neutral green energy, lignocellulose has become a good green candidate resource because of its abundant, easy-to-obtain and low-cost advantages. Sugar platform-based biorefinery technology is an effective way to utilize lignocellulose. This process mainly converts corn stalks, wheat straw and other lignocellulosic resources into polysaccharides, and then continues to convert polysaccharides into other bio-based products through biological fermentation. Among them, the annual...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/14C12R1/07C12R1/01C12R1/645
CPCC12N1/14C12N1/20C12N1/145C12R2001/645
Inventor 陈光孙旸苏瑛杰苟泽昌于潇潇
Owner JILIN AGRICULTURAL UNIV
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