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A microbial compound bacterial agent for efficiently degrading straw lignin

A technology of straw lignin and compound bacterial agent, which is applied in the direction of microorganisms, microorganisms, and methods based on microorganisms, can solve the problems of high cost, low lignin degradation rate, and less cellulose retention, and achieve industrialized utilization efficiency. Effect of Lignin Degradability

Active Publication Date: 2020-06-23
JILIN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Physical and chemical methods can reduce the wood content by about 50%, but these two methods are costly and cause secondary pollution
Microbial methods have become a research hotspot due to their simple operation, low cost and good environmental safety. However, many microorganisms can only secrete a few enzymes that degrade lignin or the enzyme activity is not high, resulting in a low lignin degradation rate and relatively low Less retained cellulose

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Example 1 Mutagenesis screening of Mycobacterium verrucosus

[0017] (1) Morphological identification and screening

[0018] The soil in the forest area of ​​Changbai Mountain Nature Reserve was collected and cultured on PDA medium by conventional methods. The mycelium of the strain was white flocculent at first, and grew divergently towards the periphery. After 8 days of culture, the color of conidia continued to deepen, and glue dots appeared; after 10 days of culture, the colonies were in the shape of concentric rings, and the conidia showed black glue dots. Light brown radial folds appear on the back.

[0019] Use a puncher to get a 1cm-diameter bacterial block, inoculate it on an aniline blue selective medium, and culture it in an aerobic incubator at 30°C for 10 days, observe the fading of aniline blue, and select a strain with a fast fading rate and strong ability as candidate strains.

[0020] At the same time, in order to directional screen lignin-degrading ...

Embodiment 2

[0026] The preparation of embodiment 2 composite bacterial agents

[0027] A microbial composite bacterial agent for efficiently degrading straw lignin. The active ingredients of the bacterial agent are the following raw materials in mass percentage: 40% of laccase-producing Myrothecium verrucosus, 30% of lignin peroxidase-producing Bacillus colioids , Manganese peroxidase-producing Actinomycetes 30%, among the above bacterial agents:

[0028] The lignin peroxidase-producing colloid bacillus (Bacillus mucilaginosus Krassilnikov) GIM 1.16;

[0029] The manganese peroxidase-producing actinomycetes (Laceyella tengchongensis) CCTCC AA208050 Laceyella; the strains described in the present invention can be purchased from China Center for Type Culture Collection (CCTCC) and Guangdong Microbiology Collection Center (GIM) get.

[0030] The laccase-producing Myrothecium verrucaria T2901 (Myrothecium verrucaria) mutant strain T2901 has a preservation number of CCTCC NO: M2017413;

[0...

Embodiment 3

[0033] Example 3 Degradation of straw

[0034] The degradation effect of strains on common corn stalks was studied by the method of solid fermentation in a triangular flask. Each 300mL Erlenmeyer flask

[0035] 5g of straws that have been dried and shredded to a width of 0.5 cm and a length of 1-2 cm, (NH 4 ) 2 SO 4 0.2g, MgSO 4 ·7H 2 O 0.05g, add 15mL of phosphate buffer solution of 5mM pH 7.0 to each bottle. Each bottle was inoculated with 0.25mL 2.0×10 8 A / mL concentration of the compound bacterial agent suspension, and at the same time take the same amount of sterile water in the control group, mix it and place it at a constant temperature of 20 ℃ for incubation. Regularly observe the morphological changes of the tobacco stalks, and wash them off after 7 days to measure the lignin degradation rate of the corn stalks.

[0036] Drawing of lignin standard curve: prepare 10g / L lignin solution, scan and select the measurement wavelength. Lignin solutions with concent...

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Abstract

The present invention provides a microbial compound microbial agent for efficiently degrading straw lignin. Mutual symbiosis and synergistic effect among bacterial groups can simultaneously secrete three enzymes related to lignin degradation in the process of fermenting straw. The laccase, lignin The activities of lignin peroxidase and manganese peroxidase were 23.56U / g, 23.02U / g and 21.74U / g biomass respectively. After fermentation, the lignin content in corn straw decreased by 60.21%, and there was no cellulose and loss of sugars such as hemicellulose. The conversion rate of cellulose in the pretreated corn stalks increased by 142.14% compared with the untreated corn stalks. The screened compound strains have strong lignin degradation ability, and the industrial utilization efficiency of the straw pretreated by the strains is significantly improved. This study provides a new pretreatment approach for the enzymatic hydrolysis and saccharification of lignocellulose in straw.

Description

technical field [0001] The invention relates to a microbial compound bacterial agent for efficiently degrading straw lignin, which belongs to the intensive agricultural production technology, and is specially used for degrading waste corn straws to produce organic fertilizers and realize resource utilization of organic wastes. Background technique [0002] The shortage of chemical energy and the increasingly serious environmental pollution make people constantly seek renewable green energy. As a carbon-neutral green energy, lignocellulose has become a good green candidate resource because of its abundant, easy-to-obtain and low-cost advantages. Sugar platform-based biorefinery technology is an effective way to utilize lignocellulose. This process mainly converts corn stalks, wheat straw and other lignocellulosic resources into polysaccharides, and then continues to convert polysaccharides into other bio-based products through biological fermentation. Among them, the annual...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/14C12R1/07C12R1/01C12R1/645
CPCC12N1/14C12N1/20C12N1/145C12R2001/645
Inventor 陈光孙旸苏瑛杰苟泽昌于潇潇
Owner JILIN AGRICULTURAL UNIV
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