Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Tea tree in-regulating growth promoting agent and application method in expediting sprouts of field tea trees

A growth-promoting agent, tea tree technology, applied in application, biocide, horticultural methods, etc., can solve problems such as the application of endophytic spirochetes not involved in tea trees, and achieve the effects of improving disease resistance, easy operation, and promoting rapid growth.

Active Publication Date: 2015-11-11
HUBEI UNIV
View PDF3 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The literature "Microbiological characteristics of two tea tree endophytic Helicospirosis" (Wang Ting et al., Journal of Microbiology 2014 No. 4, 2014.04.04, P424-432) described in detail the screening and microbiological characteristics of tea tree endophytic Helicospirosis , but did not involve the application of endophytic Helicospira in tea trees

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Tea tree in-regulating growth promoting agent and application method in expediting sprouts of field tea trees
  • Tea tree in-regulating growth promoting agent and application method in expediting sprouts of field tea trees

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Inoculate 5 mL of nutrient broth medium (10 g of tryptone, 3 g of beef powder, 5 g of NaCl, 10 μg / mL of spectinomycin, 5 μg / mL of ampicillin, and add water to 1000 ml) with the endophytic Helicospirinus strains of tea trees preserved at -80 °C. , shake at 150 rpm, and incubate overnight at 37°C. Inject the activated bacterial solution into fresh LB liquid medium (10g tryptone, 5g yeast extract, 10gNaCl, 10μg / mL spectinomycin, 5μg / mL ampicillin at a ratio of 1:100, add water to 1000ml, pH7. 2-7.4) for scale-up cultivation, the cultivation conditions are as follows: the rotation speed of the shaker is 200 rpm, and the temperature is 28-37°C. After bacterial culture for 6-8 hours, OD 600 When the value is 1.0-1.2, collect all the bacterial culture solution, add 1% glycerol, store at 4°C for later use or use directly. When in use, dilute the amplified cultured tea tree endospira spirulina bacteria solution with water at a ratio of 0.5-2:1 to become a growth promoter.

Embodiment 2

[0025] Example 2: 3000mL tea tree internal regulation and growth promoting agent liquid diluted 1:2 was sprayed on 50 square meters of cut Echa No. 1 tea tree, and the new buds of the tea tree after 60 days grew well, the leaves were dark green, and the average length of new buds increased compared with the control group 3.6cm, and the weight of 100 new branches increased by 14.6 grams compared with the control group. (See figure 1 and 2 ).

Embodiment 3

[0026] Example 3: 3000mL of tea tree internal regulation and growth promoting agent liquid diluted 1:1 was sprayed on 50 square meters of cut Echa No. 1 tea tree, and after 60 days, the new buds of the tea tree grew well, the leaves were dark green, and the average length of new buds increased compared to the control group 5.1cm, and the weight of 100 new branches increased by 19.7 grams compared with the control group. (See figure 1 and 2 ).

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a preparation method and an application method for a tea tree in-regulating growth promoting agent. By virtue of the biological characteristics of endogenous herbaspirillum seropedicae of the tea trees, the endogenous herbaspirillum seropedicae is fermented to prepare a tea tree endogenous herbaspirillum seropedicae culture solution and is sprayed to trimmed tea tree branches after being diluted in a certain proportion. Bacteria in the growth promoting agent enter into the tea tree branches for mutualistic symbiosis and secretion and metabolism of active substances so as to promote rapid growth of tea tree sprouts. The treated tea tree sprouts grow vigorously, the tea leaves are dark green, and the length and weight of the sprouts are obviously increased. The technical method provided by the invention is low in cost, simple to operate, green and safe, multiple in function and durable in effect.

Description

technical field [0001] The invention relates to a growth-promoting agent for internal regulation of new shoots of plants, in particular to a method for promoting the growth of side buds of new shoots of field tea trees by using endophytic spirulina. Background technique [0002] Tea tree is an important economic crop in my country, and it is cultivated in various provinces in southern China. After the tea leaves are picked, they can be made into green tea, white tea, yellow tea, black tea and black tea through a certain process, and the tea branches can be made into stem tea. In addition to being used as a drink, the active ingredients of tea can also be used in medicine, food, chemical industry, etc. Tea tree is a perennial economic crop whose main object is to harvest young shoots and leaves. New green and nutritious young shoots can be picked from tea trees many times a year. According to the season of picking, there are usually spring tea, summer tea and autumn tea. P...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A01N63/02A01P21/00A01G7/06
Inventor 王行国占桂婷李亚东程伟丁坤明饶辉福
Owner HUBEI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com