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Environment-friendly enzyme starter and preparation method thereof

An environmentally friendly enzyme and starter technology, applied in the direction of fungi, bacteria, etc., can solve single problems

Active Publication Date: 2017-10-24
贵州天保生态股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In summary, the environmentally friendly enzymes in the prior art are all naturally fermented, or fermented with a single strain, or fermented in stages with different strains. At present, there is no environmentally friendly enzyme prepared by fermentation with a flora starter

Method used

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  • Environment-friendly enzyme starter and preparation method thereof

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Effect test

Embodiment 1

[0027]Weigh 50g of plant roots, add it to a triangular flask containing 200mL of sterile water, shake for 5 minutes, so that the flora is evenly dispersed in the sterile water, which is the microbial flora suspension, and take 0.5mL of microbial flora respectively. The suspension was inoculated into beef extract peptone solid plate medium, starch agar solid plate medium and Martin's agar plate medium, and cultivated in a 28°C incubator until colonies grew. The microorganisms were expanded by using the corresponding liquid medium, inoculated into the fruit peel and vegetable mixture for liquid fermentation, fermented under aerobic conditions for 3 days and then centrifuged and concentrated, and spray-dried to obtain three kinds of solid bacteria powders after adding resistant starch; According to the formula ratio, 50 parts of solid bacterial powder, 20 parts of solid fungus powder, and 5 parts of solid bacteria powder of actinomycetes were added to the mixed medium after being ...

Embodiment 2

[0035] Weigh 100g of plant roots, add it to a triangular flask containing 400mL of sterile water, shake for 8 minutes, so that the flora is evenly dispersed in the sterile water, that is, the microbial flora suspension, take 1mL of microbial flora suspension The liquid was inoculated into beef extract peptone solid plate medium, starch agar solid plate medium and Martin's agar plate medium, and cultivated in a 30°C incubator until colonies grew, and picked Microorganisms, using the corresponding liquid medium to carry out bacterial culture expansion, inoculated into the fruit peel and vegetable mixture for liquid fermentation, fermented for 4 days under aerobic conditions, centrifuged and concentrated, and spray-dried after adding resistant starch to obtain three kinds of solid bacteria powder; In the formula ratio, 60 parts of solid bacterial powder, 15 parts of solid fungus powder, and 10 parts of solid bacteria powder of actinomycetes were added to the mixed medium after bei...

Embodiment 3

[0043] Weigh 100g of plant roots, add it to a triangular flask containing 400mL of sterile water, shake for 10 minutes, so that the flora is evenly dispersed in the sterile water, that is, the microbial flora suspension, take 1mL of microbial flora suspension The liquid was inoculated into beef extract peptone solid plate medium, starch agar solid plate medium and Martin's agar plate medium, and cultivated in a 30°C incubator until colonies grew, and picked Microorganisms, using the corresponding liquid medium to carry out bacterial culture expansion, inoculated into the fruit peel and vegetable mixture for liquid fermentation, fermented for 5 days under aerobic conditions, centrifuged and concentrated, and spray-dried after adding resistant starch to obtain three kinds of solid bacteria powder; In the formula ratio, 70 parts of solid bacterial powder, 10 parts of solid fungus powder, and 15 parts of solid bacteria powder of actinomycetes were added to the mixed medium after be...

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Abstract

The invention discloses an environment-friendly enzyme starter and a preparation method thereof. The preparation method includes: screening and separating three kinds of strains of bacteria, fungus and actinomycetes from plant rhizosphere microorganisms; performing spreading cultivation of the strains, liquid state fermentation, centrifugal concentration and spray drying to obtain three kinds of solid bacteria powder, activating the solid strain powder prior to inoculating the same to a mixture medium for spreading cultivation, and after the three bacterial colonies are mutually nutrient and stable in state and subjected to spreading cultivation of the bacterial colonies, adding a protective agent prior to freeze drying to obtain the environment-friendly enzyme starter. The environment-friendly enzyme starter comprises, by weight, 50-70 parts of the solid bacterial powder, 10-20 parts of the solid fungus powder, 5-15 parts of the solid actinomycetes powder, 3-10 parts of the protective agent and 5-10 parts of a fermentation promoter. Mutualistic symbiosis of the different strains is broken through, the microbial colonies are mutually nutrient and stable in state due to composition of the different microbial strains, different microorganisms have different efficacies in environment-friendly enzyme, and function of the various microorganisms can be perfected.

Description

technical field [0001] The invention belongs to the field of microbial starter, and in particular relates to a starter for environment-friendly enzymes and a preparation method thereof. Background technique [0002] At present, the environmentally friendly enzymes on the market are the common name for the brown liquid produced by natural fermentation, such as sugar, water, fruit peel, and kitchen waste. Enzyme, English name enzyme . Enzyme is another name for enzyme in Japan and Taiwan, China, and refers to a polymer substance with biocatalytic function. Almost all cellular processes require the participation of enzymes to improve efficiency. Similar to other non-biological catalysts, enzymes speed up reactions by lowering the activation energy of chemical reactions. Most enzymes can increase the rate of the reactions they catalyze by millions of times; in fact, enzymes provide another pathway with lower activation energy requirements, allowing more reactive particles to ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/14
CPCC12N1/14C12N1/20
Inventor 王兴刘先勇朱波曹彤罗敏陈旭赵伦
Owner 贵州天保生态股份有限公司
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