Quick mycorrhiza seedling cultivation method for ectotrophic mycorrhiza fungi

A technology of ectomycorrhizae and cultivation methods, applied in the direction of botany equipment and methods, cultivation, plant cultivation, etc., to achieve the effect of simple operation, high efficiency and short time consumption

Inactive Publication Date: 2020-07-03
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In general, mycorrhizal seedlings can be obtained through spore infection or mycelium inoculation, but currently only a few ectomycorrhizal fungi can be purified and cultured, so how to efficiently and quickly obtain various ectomycorrhizal fungi Mycorrhizal seedlings have become a bottleneck in the application of mycorrhizal seedlings in adversity improvement

Method used

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  • Quick mycorrhiza seedling cultivation method for ectotrophic mycorrhiza fungi
  • Quick mycorrhiza seedling cultivation method for ectotrophic mycorrhiza fungi
  • Quick mycorrhiza seedling cultivation method for ectotrophic mycorrhiza fungi

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0041]The preparation of above-mentioned mycorrhizal seedling cultivation substrate: excavate the forest topsoil of the ectomycorrhizal fungus of field purpose, soil is crushed the sieve of 1cm aperture, then add perlite or calcined core soil and mix (calcined core soil: forest top soil=2: 1); wherein the calcined core soil is a non-nutrient soil with a pH of 5.5-6.0, which is calcined at a high temperature into particles (1.5-3 mm in diameter).

[0042] Preparation of the mycorrhizal seedling cultivation substrate sterilized by high temperature and high pressure: After adding a small amount of water to the mycorrhizal seedling cultivation substrate and mixing, carry out 121°C, 1.2pa, 3h high temperature and high pressure steam sterilization.

Embodiment 1

[0044] The mycorrhizal seedlings of Russula cyanoxantha were inoculated with aseptic C.

[0045] The Stropharia mycorrhizae used in the experiment were collected from the Tianwu practice forest of the University of Tokyo, Midoricho, West Tokyo City, Tokyo, Japan (such as figure 2 shown), the host is Quercus myrsinifolia, and the host seedlings used in this inoculation experiment are 6-month-old sterile seedlings of the broad-leaved species of Fagaceae Miracle.

[0046] (1) Sterilize the aseptic seedling cultivation soil with steam under high temperature and high pressure at 121° C., 1.2 Pa, for 3 hours to obtain a sterile cultivation substrate, wherein the sterile seedling cultivation soil is selected from vermiculite or mycorrhizal seedling cultivation substrate.

[0047] (2) Disinfection of host seeds: use 30% H 2 o 2 The solution was soaked for 1 hour, then cleaned with tap water, and finally soaked in tap water overnight for later use.

[0048] (3) Cultivation and trea...

Embodiment 2

[0053] Mycorrhizal seedlings formed by inoculating aseptic rice seedlings with Russula mariae mycorrhizae

[0054] The mycorrhizae of Russula japonica used in the experiment were collected from the field training forest of Tokyo University, Midoricho, Nishitokyo City, Tokyo, Japan (such as Figure 4 shown), and its host is Castanopsis militaris. The host seedlings used in this inoculation experiment were 6-month-old sterile seedlings of Castanopsis oryzae.

[0055] (1)-(5) are the same as embodiment 1

[0056] (6) Formation of mycorrhizae of Russula japonica: the aseptic seedlings inoculated with mycorrhizae formed mycorrhizae during the infection process of 3 months, and successfully infected the roots of aseptic seedlings to form a large number of mycorrhizal seedlings (as shown in the attached Figure 4-5 shown).

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Abstract

The present invention relates to the technical field of forestry mycorrhiza seedling cultivation, and particularly relates to a quick mycorrhiza seedling cultivation method for ectotrophic mycorrhizafungi. The method comprises the following steps of a, performing high-temperature and high-pressure sterilization on aseptic seedling cultivation soil; b, performing surface disinfection on host plantseeds by a disinfectant, sowing the host plant seeds in the sterilized aseptic seedling cultivation soil, continuing cultivation for 30-45 days after germination, and transplanting the host plant seeds into an aseptic container loaded with a mycorrhiza seedling cultivation matrix subjected to high-temperature and high-pressure sterilization; c, selecting high-activity mycorrhizae, removing surface soil, washing the mycorrhizae with tap water, and finally washing the mycorrhizae with aseptic water; d, attaching the washed mycorrhizae to a host seedling root system, and performing cultivation in a greenhouse or a cultivation box to form mycorrhiza seedlings; and e, selecting the mycorrhiza seedlings with good mycorrhiza formation as infected parent seedlings, and performing co-cultivation on the infected parent seedlings and aseptic seedlings or the disinfected host plant seeds in the aseptic mycorrhiza seedling cultivation matrix to obtain mycorrhiza seedlings of a large amount of theectotrophic mycorrhiza fungi.

Description

technical field [0001] The invention relates to the technical field of forestry mycorrhizal seedling cultivation, in particular to a method for rapidly cultivating mycorrhizal seedlings of ectomycorrhizal fungi. Background technique [0002] Ectomycorrhizal fungi generally form a rhizosphere symbiosis system with higher host plants. In the huge forest rhizosphere network system, host plants provide carbohydrates to ectomycorrhizal fungi, which in turn help host plants Absorb water and mineral elements in the soil and promote the secretion of secondary metabolites that are beneficial to the growth and development of host plants. Many ectomycorrhizal fungi can not only promote the growth of host plants and improve their ability to resist adversity, but also their fruiting bodies have high edible and medical value, such as truffles (Tuber spp.) of the genus Truffles, and truffles of the genus Boletus. Boletusedulis, Tricholoma matsutake, Russula griseocarnosa, Russula altacea,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G18/10A01G17/00
CPCA01G17/005A01G18/10
Inventor 练春兰余伟满家银张桃香吴松青胡霞
Owner FUJIAN AGRI & FORESTRY UNIV
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