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A kind of ectomycorrhizal fungus with cr(vi) tolerance and reducing ability and its application

An ectomycorrhizal and capacity technology, applied in the field of environmental pollution control, can solve the problems of secondary pollution, incomplete removal, affecting the restoration efficiency, etc., and achieve the effect of high reduction efficiency and improvement of restoration efficiency.

Active Publication Date: 2021-12-21
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Based on these two ideas, the following traditional remediation technologies for chromium-contaminated soils have been derived: (1) Immobilization and stabilization technologies: high cost, insufficient treatment effect, and incomplete removal
(2) Chemical reduction technology: causing secondary pollution, lack of technical support
(4) Electric repair technology: harsh conditions and high cost
(5) Phytoremediation technology: the plant type is small, the root system is shallow, and the growth is slow, which affects the restoration efficiency
However, there is no research report on the application of ectomycorrhizal fungi to the remediation of chromium-contaminated soils.

Method used

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  • A kind of ectomycorrhizal fungus with cr(vi) tolerance and reducing ability and its application
  • A kind of ectomycorrhizal fungus with cr(vi) tolerance and reducing ability and its application
  • A kind of ectomycorrhizal fungus with cr(vi) tolerance and reducing ability and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Example 1 Bacterial Colony Characteristics and Physiological and Biochemical Characteristics

[0048] Strain Isolation: Pisolithus sp.T Cr -1 (CCTCC NO: M2018087) was isolated from the fruiting body collected in Sanqingshan (28.54N118.03E) in Jiangxi Province, NCBI Genbank accession number: KY075875, and the ITS sequence is shown in SEQ ID No.1. Bacteria, colony morphology and physiological and biochemical characteristics: ectomycorrhizal fungi, non-spore-forming filamentous fungi, mycelium yellowish yellow, aerobic, plant symbiotic bacteria.

Embodiment 2

[0049] Example 2 Pisolithus sp.T Cr -1 (CCTCC NO:M2018087) Cr(VI) resistance test

[0050] Pisolithus sp.T activated in the dark at 25°C for 20 days Cr -1 (CCTCC NO: M2018087) use a 0.65cm diameter hole puncher to make a plaque along the edge and inoculate it in the center of the solid medium with a final concentration of Cr(VI) of 0, 2, 5, 10, 50, 55, 60ppm , Measure the plaque diameter every 3 days with the cross method, and finish the measurement after 18 days, and calculate its half-inhibitory concentration to Cr(VI). The experimental results can be concluded that low concentration (0-10mg / L) Cr(VI) treatment inhibits Pisolithus sp.T Cr -1 (CCTCC NO:M2018087) growth but not significantly; high concentration Cr(VI) treatment (50mg / L) significantly inhibited Pisolithussp.T Cr -1 (CCTCC NO:M2018087) growth, but still alive, 55mg / L for Cr(VI) on Pisolithus sp.T Cr -1 (CCTCC NO:M2018087) lethal final concentration, after calculation, Cr(VI) to Pisolithus sp.T Cr The half-i...

Embodiment 3

[0051] Example 3 Pisolithus sp.T Cr -1 (CCTCC NO:M2018087) Physiological Mechanism of Hexavalent Chromium Removal

[0052] The ectomycorrhizal fungus Pisolithus sp.T purely cultivated by laboratory solid Cr -1 (CCTCC NO:M2018087) to study the physiological mechanism of hexavalent chromium removal. The ectomycorrhizal fungus Pisolithus sp.T activated in the dark at 25°C for 20 days Cr -1 (CCTCC NO: M2018087) Use a hole puncher with a diameter of 0.80cm to make two plaques along the edge and inoculate it in a 150ml Erlenmeyer flask with 100ml Cr(VI) final concentration of 0,25,50ppm liquid medium, Shake at 150rpm at 25°C for 12 days in the dark, and take samples on days 0, 4, 8, and 12 to determine the contents of hexavalent chromium and total chromium in the medium. The content of hexavalent chromium was measured by diphenylcarbonyl spectrophotometry, and the removal rate of Cr(VI) in the medium was calculated, and the content of trivalent chromium was calculated by differen...

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Abstract

The invention provides an ectomycorrhizal fungus with Cr(VI) tolerance and reducing ability, which is classified as Pisolithus sp. and has a preservation number of CCTCC No: M2018087. The application of hexavalent chromium in contaminated soil, and the application of plant-microbe joint remediation of chromium-contaminated soil. The invention also provides a method for using the fungus to jointly restore chromium-contaminated soil by plants and microbes. The ectomycorrhizal fungus Pisolithus sp. obtained by the separation and screening of the present invention is resistant to Cr(VI) and has the ability to remove it, which is of great significance to the removal of hexavalent chromium in chromium-contaminated soil. On this basis, a plant-microbial joint The new remediation system for remediation can give full play to the advantages of both, and greatly improve the remediation efficiency of heavy metal Cr(VI) contaminated soil.

Description

technical field [0001] The invention relates to the technical field of environmental pollution control, and relates to biological materials for remediation of heavy metal polluted soil. Background technique [0002] At present, the harm of heavy metal pollution to the environment and human health has attracted much attention. Among them, chromium pollution mainly comes from wastewater and waste residues in industries such as electroplating, tanning, petroleum, paint, printing and dyeing, and is also one of the common migratory pollutants in nature. The environmental chromium pollution caused by the landfill and stockpiling of chromium slag has become a serious problem. Chromium usually exists in two stable valence states of Cr(VI) and Cr(III) in natural water-soil systems. Cr(VI) is a thermodynamically stable valence state with high chemical activity and strong toxicity. HCrO 4 - and CrO 4 2- It exists in two forms and is one of the main pollutants causing environmenta...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01H15/00B09C1/10C12R1/645
CPCA01H15/00B09C1/105B09C2101/00C12R2001/645C12N1/145
Inventor 陈亚华史良汤阳泽王杰蒋明里刘镔皞夏妍陈晨沈振国
Owner NANJING AGRICULTURAL UNIVERSITY
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