A method for establishing a symbiotic relationship between ectomycorrhizal fungi and Pinus plants

Abstract

The invention relates to a method for forming mycorrhizae after two types of ectotrophic mycorrhiza fungi, namely sparassis crispa and tricholoma matsutake infect seedlings of several kinds of pinus plants and belongs to the fields of artificial ectotrophic mycorrhiza fungus infection seedling-growing application technology and agricultural cultivation technology. The method comprises the steps of 1, acquisition and cultivation of sterile seeds of the pinus plants; 2, selection and treatment of a culture substrate; 3, selection and treatment of seedling-growing hole trays; 4, preparation before infection treatment; 5, infection treatment of the sparassis crispa and the tricholoma matsutake; 6, seedling stage management after infection treatment. The seedlings with the mycorrhizae successfully formed through infection grow stronger than the seedlings not subjected to infection, the leaf color is thick green, euphylla grows out in advance, disease resistance is enhanced, the symbiotic relationship between the sparassis crispa, the tricholoma matsutake and the several kinds of pinus plants under controllable conditions is creatively and successfully created, a study case can be provided for the symbiotic relationship between plants and fungi in the study of the ectotrophic mycorrhiza fungi, and a solid scientific foundation is laid for realizing artificial cultivation of the ectotrophic mycorrhiza fungi.

Description

Technical field [0001] The invention relates to a method for the formation of mycorrhizal by ectomycorrhizal fungi such as dry bacillus, matsutake and the like infecting seedlings of several pinus plants, and belongs to the field of artificial ectomycorrhizal fungus infection and seedling breeding application technology and agricultural cultivation technology. Background technique [0002] Dry Bacteria ( Thelephoraganbajun Zang) and Matsutake (also known as Matsutake)[ Tricholomamatsutake (lto et lmai) Singer] are two famous and delicious wild mushrooms. They have high nutritional value and medicinal value. They are deeply loved by people. With the improvement of people's living standards, the current market is in short supply, expensive and dry. Fresh bacteria weight is the most expensive up to 1,800 yuan per 1 Kg. Dry scallops are rich in fragrance, tender and sweet, tough and dry, with a mellow and long aftertaste, endless chewing, and have a strong aroma that resembles corne...

AI Technical Summary

Problems solved by technology

[0004] The two wild fungi, Ganba and matsutake, are typical ectomycorrhizal fungi, which cannot be cultivated artificially at present, and are difficult to develop and utilize. The growth and development of their fruiting bodies are closely related to the symbiotic plants of the genus Pinus.