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Fast reproduction process of Ma Hali cherry stock

A technique for mahali and rootstocks, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the difficulty in rooting of mahali cherry rootstock seedlings and mohali cherry rootstock tissue culture seedlings that have not been seen in industrial production , unable to realize large-scale factory production and other problems, to achieve good multiplication and rooting effects, strong and tidy root system, and improved work efficiency

Inactive Publication Date: 2003-08-20
SHAANXI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] It is difficult for the rootstock tissue culture seedlings of Mahali cherry to take root, and the low survival rate of transplanting makes it impossible to achieve large-scale industrial production. So far, there has been no report of industrial production of Mahali cherry rootstock seedlings at home and abroad.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0075] (1) Preparation of tissue culture seedlings

[0076] Pretreatment of raw materials: Cut the healthy vegetative branches of excellent individual plants in the full fruit stage cultivated in the field without diseases and insect pests, cut off the young shoots, rinse them with running water, and dry them with clean filter paper; Disinfection of materials: rinse them on an ultra-clean workbench Soak the clean sprouts in 70% alcohol for 10-30 seconds, rinse with sterile water for 3 times, each time for 5 minutes, and then insert 0.1% HgCl 2 Sterilize in the solution for 6-8 minutes, wash the young shoots with sterile water for 3-5 times, 5 minutes each time, and finally blot them dry with sterile filter paper, insert them as explants into 1 / 2MS basic medium to make the shoots grow , transfer the buds into new 1 / 2MS basic medium every 3 to 5 days, repeat 2 to 3 times, and transfer the buds into the bud growth medium when the color of the medium no longer changes. The shoot ...

Embodiment 2

[0100] In the cultivation steps of the tissue culture seedlings of the present embodiment, the shoot growth medium used is made by adding the following raw materials and their proportioning in 1 liter of MS basic medium:

[0101] White sugar 15g

[0102] Agar powder 4g

[0103] 6-(Benzylamino)purine 0.2mg

[0104] In the cultivation steps of the tissue culture seedlings of this embodiment, the proliferation medium used is made by adding the following raw materials and their proportions in 1 liter of MS basic medium:

[0105] White sugar 15g

[0106] Agar powder 4g

[0107] 6-(Benzylamino)purine 0.3mg

[0108] Indoleacetic acid 0.3mg

[0109] Gibberellin 0.1mg

[0110] Casein 0.01g

[0111] In the rooting culture step of the tissue culture shoots, the rooting medium used is to add the following raw materials and the proportioning thereof in 1 liter of 1 / 2MS basic medium:

[0112] White sugar 15g

[0113] Agar powder 4g

[0114] Indole-3-butyric acid 0.1mg

[0115] Othe...

Embodiment 3

[0117] In the cultivation steps of the tissue culture seedlings of the present embodiment, the shoot growth medium used is made by adding the following raw materials and their proportioning in 1 liter of MS basic medium:

[0118] White sugar 80g

[0119] Agar powder 10g

[0120] 6-(Benzylamino)purine 0.6mg

[0121] In the cultivation steps of the tissue culture seedlings of this embodiment, the proliferation medium used is made by adding the following raw materials and their proportions in 1 liter of MS basic medium:

[0122] White sugar 80g

[0123] Agar powder 10g

[0124] 6-(Benzylamino)purine 4mg

[0125] Indoleacetic acid 0.9mg

[0126] Gibberellin 3mg

[0127] Casein 0.05g

[0128] In the rooting culture step of the tissue culture shoots, the rooting medium used is to add the following raw materials and the proportioning thereof in 1 liter of 1 / 2MS basic medium:

[0129] White sugar 80g

[0130] Agar powder 10g

[0131] Indole-3-butyric acid 0.9mg

[0132] Oth...

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Abstract

The fast reproduction process of Prunus mahaleb stock includes the preparation of tissue culture seedling, rooting culture of tissue culture seedling, seedling growth and hardening seeding, and transplanting. The tissue culture seedling and the rooting culture of tissue culture seedling are performed in a dark room; the seedling growth and hardening seeding is under natural lighting; and bud growing culture medium, multiplication culture medium and rooting culture medium suitable for the fast growth of Prunus mahaleb stock are used. The said process can produce robest stuggy Prunus mahaleb stock fast.

Description

technical field [0001] The invention belongs to the field of plant regeneration of plant tissue culture technology, and undifferentiated human, animal or plant cell technology, and in particular relates to a rapid propagation method of mahali cherry rootstock. Background technique [0002] Mahaleb cherry rootstock (Mahaleb) is native to central and southern Europe, and is a wild variety, mostly in the state of shrubs. The branches are taupe, the branches are thin and short, and the branching force is strong. The leaves are small, round or oval, shiny, with a smooth waxy layer on the surface. The flowers are small and white. The fruit is small, purple-black when fully ripe, and separated from the core. The pulp is inedible. The seeds are small, oval to round, and the number of seeds per kilogram is 12000-15000. After stratification treatment, the germination rate can reach more than 90%, and multi-purpose seed sowing is used for propagation. The emergence rate is high, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 王喆之肖娅萍张志勤黄馨慧
Owner SHAANXI NORMAL UNIV
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