The invention discloses a potting culture medium for clivia miniata and a preparation method thereof, and belongs to the technical field of culture. The potting culture medium comprises the followingraw materials in parts by weight: 10-30 parts of corncob particles, 10-20 parts of crushed wood dust particles, 10-15 parts of Chinese medicinal residues, 5-10 parts of fish bone meal, 5-10 parts of vinasse, 8-16 parts of plant ash, 20-30 parts of livestock manure, 3-6 parts of glutinous rice, 1-3 parts of a tourmaline powder negative ion additive, 14-25 parts of attapulgite clay, 1-3 parts of potassium sulfate, 0.1-0.5 part of aspartic acid, 2-4 parts of urea, 0.2-0.5 part of glycine, 10-20 parts of perlite, 0.1-0.4 part of indoleacetic acid, 1-3 parts of boric acid, 0.1-0.4 part of gibberellin, 3-5 parts of a microbial fermentation bacterial agent and 1-3 parts of active carbon. The preparation method of the potting culture medium is simple; and the potting culture medium provided by theinvention has the characteristics of loose texture, ventilation, water permeability, fast rooting, shortened cultivation time and the like, and can be widely applied to potted flower cultivation.