Application of saturated acetylene aqueous solution in promoting flowering of clivia miniata

Abstract

The invention belongs to the technical field of flowering phase regulation and control of ornamental plants, and particularly relates to application of a saturated acetylene aqueous solution in promoting flowering of clivia miniata, and 200-600 mL of the saturated acetylene aqueous solution is applied to the clivia miniata every time in the growing period in winter. Calcium carbide is rapidly decomposed into acetylene and calcium hydroxide when encountering water, the reaction is violent, a large amount of acetylene generated by the reaction is dissolved in water, namely the calcium carbide aqueous solution can obtain an acetylene saturated solution, and the saturated acetylene aqueous solution does not have an inhibition effect on growth of pineapples, but does not have an obvious inhibition effect; the saturated acetylene aqueous solution can promote the flowering of the clivia miniata, so that the method for promoting the flowering of the clivia miniata by using the saturated acetylene aqueous solution is provided.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of ornamental plant flowering regulation, and particularly relates to application of saturated ethyne aqueous solution in promoting Clivia nobilis to bloom. BACKGROUND

[0002] Clivia nobilis Lindl. Clivia miniata Clivia nobilis Lindl. is a perennial evergreen plant of the genus Clivia in the Amaryllidaceae family. Clivia nobilis Lindl. has beautiful flower shape, erect leaf shape and evergreen leaf color. The inflorescence structure of Clivia nobilis Lindl. originates from a small group of cells in the stem apical meristem, which are transformed into the apical meristem. Secondly, during the flowering process, monopodial branching and sympodial branching are the two main modes of inflorescence structure. The first stage: the monopodial branching mode is followed before the 12th leaf is produced. The second stage: when Clivia nobilis Lindl. has four new leaves, it is sympodial branching, the terminal bud continues to elongate and new flower buds are produced.

[0004] Through observation and analysis of the flowering characteristics of Clivia nobilis Lindl. in Sanming, it is found that Clivia nobilis Lindl. flower bud differentiation is based on nutrient accumulation, and low temperature treatment is necessary to complete flowering induction, and the quality of flowering is closely related to flower bud differentiation. Our previous studies have also shown that Clivia nobilis Lindl. is a type of multiple flower bud differentiation, and more than 4-year-old Clivia nobilis Lindl. has 2 inflorescences, but usually only one peduncle can be extracted. There is a direct relationship between the number of leaves and flower buds. After 12 leaves of Clivia nobilis Lindl. grow, a flower bud will sprout after every 4-5 new leaves grow. The origin of flowering of Clivia nobilis Lindl. is the apical meristem of its flower stem, which undergoes limited division and finally differentiates into flower buds, thereby developing flower organs; secondly, the stems of the plants also begin to grow and develop, and finally the phenomenon of Clivia nobilis Lindl. arrow flowering occurs. To make Clivia nobilis Lindl. bloom before the Spring Festival, it needs to be treated at 10℃ in early September to stimulate Clivia nobilis Lindl. to extract the flower stem in advance. In addition to low temperature treatment, the use of plant exogenous hormones has always been a recognized effective means of regulating plant flowering.

[0005] As an analogue of ethylene (acetylene), acetylene is deeply involved in the hormone regulation process in plants, and thus profoundly affects the overall life course and morphological construction of plants. The prior art finds that treating pineapple with acetylene saturated aqueous solution has the effect of promoting the flowering period. It may achieve this by interfering with the hormone balance in the pineapple and promoting the synthesis and signal transduction of flowering-related hormones, thereby promoting pineapple flowering and advancing the flowering period. However, due to the differences in characteristics between plants and varieties, it is unknown whether acetylene has the effect of promoting the flowering of Clivia nobilis Lindl. SUMMARY

[0006] In order to solve the above technical problems, the application provides application of saturated acetylene aqueous solution in promoting flowering of Clivia.

[0007] Application of saturated acetylene aqueous solution in promoting flowering of Clivia.

[0008] Preferably, 200 mL-600 mL of saturated acetylene aqueous solution is applied to Clivia each time during the winter growth period; When applied, it is applied every 2 days, and after 7 times of spraying, it is applied once a week, and after 4 times, the application is stopped.

[0009] Preferably, the application is spraying to the leaves of Clivia and root irrigation of Clivia.

[0010] Preferably, the volume ratio of spraying to the leaves of Clivia to root irrigation of Clivia is 1-3:3-5.

[0011] Preferably, the volume ratio of spraying to the leaves of Clivia to root irrigation of Clivia is 2:3.

[0012] Preferably, the first application time is November.

[0013] Preferably, the first application time is from November 1 to November 10.

[0014] Preferably, after applying saturated acetylene aqueous solution during the winter growth period, saturated acetylene aqueous solution is applied during the summer dormancy period.

[0015] Preferably, the application starts from April to May, acetylene saturated aqueous solution is applied every 3 days, after 7 times of application, the application frequency is once a week, and the application is 8 times.

[0016] Compared with the prior art, the application has the beneficial effects that: Calcium carbide (calcium carbide) is rapidly decomposed into acetylene and calcium hydroxide when it comes into contact with water, and the reaction is violent. The acetylene produced in the reaction can be dissolved in water in large amounts, that is, calcium carbide aqueous solution can obtain acetylene saturated solution. The application finds that due to the differences in characteristics between plants and varieties, the flower-promoting effect of saturated acetylene aqueous solution on pineapple growth is not universal. When the flower-promoting effect of saturated acetylene aqueous solution on 5 pineapple varieties, namely, red songbird, jili red star, dennis, medium torch, and large torch, changes with different application conditions, there is no obvious flower-promoting effect on pinecone pineapple and red star pineapple. It is shown that acetylene saturated aqueous solution does not exert flower-promoting effect on all plants. The application finds that saturated acetylene aqueous solution can promote flowering of Clivia, and therefore proposes application of saturated acetylene aqueous solution in promoting flowering of Clivia. BRIEF DESCRIPTION OF DRAWINGS

[0017] Figure 1The number of new leaves of Clivia nobilis treated with acetylene during the growth period was observed.

[0018] Figure 2 The growth speed of leaves of Clivia nobilis during the dormant period was observed, A is the length of the tender leaf blade treated with acetylene; B is the number of new leaves treated with acetylene.

[0019] Figure 3 The flowering-related parameters of Clivia nobilis treated with acetylene and fertilizer, A is the flowering order and flowering time; B is the flowering rate.

[0020] Figure 4 The flowering-related parameters of Clivia nobilis treated with acetylene and fertilizer, A is the flowering order and flowering time; B is the flowering rate. DETAILED DESCRIPTION

[0021] The specific embodiments of the present application are described in detail below, but it should be understood that the protection scope of the present application is not limited by the specific embodiments. Based on the examples in the present application, all other examples obtained by those of ordinary skill in the art without making creative efforts fall within the scope of protection of the present application. The experimental methods described in the embodiments of the present application are conventional methods unless otherwise specified.

[0022] Although the prior art discloses that acetylene saturated aqueous solution has good flower induction effect on ornamental pineapple and other plants, there are still cases where the flower induction effect is poor due to improper treatment methods and other factors. Relevant examples are as follows: the heart of medium torch and large torch pineapple was irrigated 3 times without effect.

[0023] (1) According to the article “Effect of Acetylene Gas Saturated Aqueous Solution on Flowering Period of Pineapple” (DOI: 10.15889 / j.issn.1002-1302.2014.11.076), flower induction tests were conducted on 5 pineapple varieties including Hongyingge, Jilihongxing, Dennis, medium torch and large torch. It was found that acetylene gas saturated aqueous solution irrigated 3 times only had obvious effect on Hongyingge, Jilihongxing and Dennis, and had no effect on medium torch and large torch with long growth period. Irrigation 4 times also had general effect on medium torch and large torch, and irrigation 5 times was needed to have better flower induction effect.

[0024] (2) “Research on Flower Induction Technology of Ornamental Pineapple” (https: / / www.renrendoc.com / paper / 156134186.html) discloses the influence of different types and concentrations of flower induction agents on the flower induction effect of ornamental pineapple. The results show that: ethylene has inhibitory effect on the growth of 2 kinds of pineapple, and the flower induction effect of ethylene solution on pinecone pineapple is not obvious, and the flower induction effect on Hongxing pineapple is better, and the best treatment concentration is 400 mg / kg; calcium carbide aqueous solution does not produce inhibitory effect on the growth of 2 kinds of pineapple, but has no obvious flower induction effect.

[0025] (3) Zhang Ximin, Yi Yin, Yang Hua, Gao Wei, Wang Mingyong. Effects of exogenous hormones on the regulation of flowering period of Rhododendron dewdrop[J]. Northern Horticulture, 2009, (12): 170-173. Using Rhododendron dewdrop as the experimental material, the study was carried out by spraying different concentrations of ethephon. The results showed that ethephon treatment had no significant effect on the flowering period of Rhododendron dewdrop.

[0026] (4) Cheng H, Zhou M, Si YY, Li WJ, Wang LK, Chen SM, Chen FD, Jiang JF. Transcriptome analysis of ylene response in Chrysanthemummoriflolium Ramat. with an emphasis on flowering delay[J]. Horticulturae, 2023, 9 (4):428. Chrysanthemum was used as the experimental material and treated with ethephon. The results showed that ethephon inhibited flowering of chrysanthemum.

[0027] In summary, due to the varietal characteristics between species, saturated aqueous solutions of acetylene do not induce flowering in all plants. Similarly, ethephon, a similar compound to acetylene, does not induce flowering in all plants. Therefore, the effects of saturated aqueous solutions of acetylene and ethephon on other plants cannot be used to predict the flowering effect of Clivia miniata.

[0028] This invention involves applying 200 mL to 600 mL of saturated acetylene aqueous solution to Clivia miniata during its winter growing season. The application is repeated every two days for seven applications, then weekly for four applications, after which application is discontinued. Results showed that the acetylene treatment exhibited the earliest onset of flowering, on January 7, 2024, with an average onset date of January 18, 2024. Furthermore, the first plant in this treatment flowered on January 26, 2024, 15 days earlier than the control group (CK), and earlier than both the acetylene and fish protein treatment groups and the acetylene and potassium dihydrogen phosphate treatment groups.

[0029] Example 1 A method to promote flowering of Clivia miniata includes the following steps: Starting from November 8, 2023, spray each large-flowered clivia plant with 500 mL of saturated acetylene solution, of which 200 mL is used for foliar spraying and 300 mL is used for root irrigation. Spray once every 2 days for 7 times, then spray once a week, and stop spraying after 4 times.

[0030] From April 18, 2024 to May 12, 2024, a treatment solution of saturated acetylene was sprayed every 3 days (spraying method was the same as the winter treatment), for a total of 7 times. From May 19, 2024 to July 7, 2024, the spraying frequency was once a week, for a total of 8 times. After spraying with the saturated acetylene solution, the flowering status was observed.

[0031] Effect verification Acetylene treatment regulates the growth and related physiological indicators of Clivia miniata. 1. Observation of leaf growth rate From October 24, 2023 to February 2, 2024, the growth of Clivia miniata leaves and the number of new leaves were observed and recorded weekly during the winter growing season. Starting from November 8, 2023, each pot of Clivia miniata was sprayed with a treatment solution of 500 mL of saturated acetylene water, of which 200 mL was used for foliar spraying and 300 mL was used for root irrigation. Spraying was done once every 2 days (d) for 7 times, then once a week for 4 times, and then spraying was stopped. The growth and flowering status were observed.

[0032] Since the treatment of each large-flowered clivia with 500 mL of acetylene saturated aqueous solution on November 8, 2023, from August 1, 2024 to September 5, 2024, the growth status of the large-flowered clivia leaves in terms of length, width and number of new leaves was observed and recorded once a week during the summer dormancy period.

[0033] When measuring leaves, the two leaves closest to the flower stalk should be used as the reference, and the leaf length should be measured from the base to the tip. For recording leaf width, the widest part of the leaf should be selected. Furthermore, the calculation of new leaf growth is based on the number of visible leaves.

[0034] Results: During the winter growing season, the leaf growth rate of acetylene-treated Clivia miniata was significantly higher than that of the control group (CK group). Figure 1 (B) Compared to the control group (CK), the leaf width was wider in all groups, but the growth was slower and there was no significant difference. The number of new leaves was higher in all groups compared to the control group, and the highest growth rate of new leaves occurred from January 3rd to January 10th of the following year. Figure 1 (A in the middle).

[0035] During the summer dormancy period, the leaf growth ratio of acetylene-treated Clivia miniata was higher than that of the control group (CK group). Figure 2 (B) Compared to the control group (CK), the leaf width was wider in all groups, but the growth was slower and there was no significant difference. The number of new leaves was higher in all groups compared to the CK group, with a higher increase in the number of new leaves from August 15 to September 5. Figure 2 (A in the middle).

[0036] 2. Observation of flowering time (1) Under the conditions of spraying acetylene and adding KH2PO4 and fish protein respectively during the winter growing season, according to Figure 3 The following analysis will be conducted.

[0037] Forty potted Clivia miniata plants were used for winter growing season treatments. Ten pots were set as the control group (CK), ten pots were sprayed with a saturated aqueous solution of acetylene, ten pots were sprayed with a solution of acetylene and KH2PO4 (KH2PO4 final concentration was 0.005 g / L), and ten pots were sprayed with a solution of acetylene and fish protein (fish protein concentration was 1000 times).

[0038] Starting from November 8, 2023, each group of large-flowered Clivia plants was sprayed with 500 mL of the corresponding treatment solution, of which 200 mL was used for foliar spraying and 300 mL was used for root irrigation. Spraying was done once every 2 days for 7 times, then once a week for 4 times, and then spraying was stopped. The flowering status was observed.

[0039] The results showed that the acetylene treatment had the earliest inflorescence date, January 7, 2024, and the first plant in this treatment flowered on January 26, 2024, 15 days earlier than the control group. The average inflorescence date for the acetylene treatment was January 18, 2024. Figure 3 (A in the middle).

[0040] Further observation of flowering rate ( Figure 3 The percentages of B in the treatment were 80%, 65%, and 55%, respectively. Overall, acetylene showed the best effect in terms of flowering performance. Moreover, when comparing the initiation time and flowering rate, all three treatments were superior to the CK group.

[0041] (2) Based on the experimental results obtained after spraying treatment during the winter growing season, it is clear that acetylene spraying has the best effect. Therefore, spraying experiment was conducted on Clivia miniata during its dormancy period.

[0042] From April 18, 2024 to May 12, 2024, a treatment solution of saturated acetylene aqueous solution was applied every 3 days (application method was the same as during the winter growing season), for a total of 7 times. From May 19, 2024 to July 7, 2024, the spraying frequency was once a week, for a total of 8 times. After spraying with the saturated acetylene solution, the flowering status was observed.

[0043] The results showed that the inflorescence time of the acetylene-treated group was May 13, 2024, and the first flowering time was May 24, 2024, which was 10 days earlier than that of the control group. The average inflorescence time was May 20, 2024. Figure 4 (A) Further observation of the flowering rate of each treatment ( Figure 4 (B in the text) The acetylene flowering rate is 20%.

[0044] 3. The effect of spraying time on promoting flowering Thirty potted Clivia miniata plants were treated with different spraying times, with 10 pots for each treatment. The specific spraying times were October 1, 2023, November 8, 2023, and December 1, 2023, and the spraying method was the same as above. The flowering status was observed.

[0045] Results: On November 8, 2023, the acetylene treatment showed the earliest flowering time, which was January 7, 2024, and the first plant of this treatment flowered on January 26, 2024; on December 1, 2023, the first plant flowered on February 12, 2024; and on October 1, 2023, the first plant flowered on February 18, 2024.

[0046] It should be noted that when numerical ranges are mentioned in the claims of this invention, it should be understood that the two endpoints of each numerical range and any value between the two endpoints can be selected. To avoid redundancy, the present invention describes preferred embodiments.

[0047] Although preferred embodiments of the invention have been described, those skilled in the art, upon learning the basic inventive concept, can make other changes and modifications to these embodiments. Therefore, the appended claims are intended to be interpreted as including both the preferred embodiments and all changes and modifications falling within the scope of the invention.

[0048] Obviously, those skilled in the art can make various modifications and variations to this invention without departing from its spirit and scope. Therefore, if these modifications and variations fall within the scope of the claims of this invention and their equivalents, this invention also intends to include these modifications and variations.

Claims

1. Application of saturated acetylene aqueous solution in promoting flowering of Clivia miniata.

2. The application according to claim 1, characterized in that, During the winter growing season, apply 200mL~600mL of saturated acetylene aqueous solution to the large-flowered clivia each time. When applying, apply once every 2 days. After 7 applications, apply once a week. After 4 applications, stop applying.

3. The application according to claim 2, characterized in that, The application refers to spraying the leaves of the Clivia miniata and drenching the roots of the Clivia miniata.

4. The application according to claim 3, characterized in that, The ratio of the volume of spray applied to the leaves of Clivia miniata to the volume of water applied to the roots of Clivia miniata is 1~3:3~5.

5. The application according to claim 4, characterized in that, The ratio of the volume of spray applied to the leaves of Clivia miniata to the volume of water applied to the roots of Clivia miniata is 2:

3.

6. The application according to claim 2, characterized in that, The first application was in November.

7. The application according to claim 6, characterized in that, The first application period is from November 1st to November 10th.

8. The application according to claim 1, characterized in that, After applying a saturated acetylene aqueous solution during the winter growing season, apply the same solution again during the summer dormancy period.

9. The application according to claim 8, characterized in that, Begin application in April or May, applying a saturated aqueous solution of acetylene every 3 days. After 7 applications, apply once a week for a total of 8 applications.

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