Preparation and sowing method of ectomycorrhizal fungus granule strain

Abstract

The invention discloses preparation of an ectomycorrhizal fungus granule strain. The preparation comprises the following steps: (1) stirring and smashing ectomycorrhizal fungi to obtain mycelium, so as to obtain ectomycorrhizal fungus liquid; (2) adding a nutrient solution into the ectomycorrhizal fungus liquid obtained in the step (1), so as to obtain a mixed solution; and (3) slowly adding the mixed solution obtained in the step (2) into polyacrylamide, continuing to stir until the mixed solution is completely adsorbed, then adding wheat bran powder, and continuing to stir, so as to obtain the ectomycorrhizal fungus granule strain. A sowing method of the ectomycorrhizal fungus granule strain is carried out by adopting inclined-plane root breaking or cave-shaped root breaking. The ectomycorrhizal fungus granule strain disclosed by the invention has the advantages that the problems that the prior art is limited by distribution of target mycorrhizal fungi, the labor intensity of sowingis high and the influence of weather is larger are improved; and the used raw materials are simple and easy to obtain, the preparation method is simple, the operation is simple and convenient, the production cost is reduced, the economic benefit is increased, and the preparation is suitable for large-area popularization and application.

Description

technical field [0001] The invention belongs to the technical field of edible fungus strain seed production, in particular to a method for preparing and sowing ectomycorrhizal fungus granule strains. Background technique [0002] Many macrofungi form symbiotic relationships with the roots of higher plants, which we call ectomycorrhizae. There are extremely rich ectomycorrhizal fungi in China, and more than 600 species are known, such as Russula, Lactarum, Amanita, Boletus, and Scleroderma scleroderma in the agaric family Truffles of the genus Beanbag and Ascomycetes. The research and application of ectomycorrhizal fungi are of great significance for the development of forestry seedlings, the promotion of forest tree growth and development, and the greening of barren hills and mine waste land. Ectomycorrhizal fungi are mycorrhizal complexes formed by fungi and the roots of higher plants, and the metabolites of the host and mycorrhizal fungi operate bidirectionally through t...

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Problems solved by technology

[0003] However, ectomycorrhizal edible and medicinal fungi cannot be reproduced under purely artificial conditions. In the past, ectomycorrhizal fungus strains were sprayed on the host rhizosphere with a sprayer, which is not suitable for large-scale cultivation. There are two main factors for large-scale promotion and application: (1) high labor intensity, and the input-output ratio is not suitable for large-scale promotion and application; (2) in case of drought, the activity of bacteria will be greatly reduced

According to previous research data, they were all limited to small-scale experiments, and many factors such as strain application and management were not considered. The above two factors are a major bottleneck for the large-scale promotion and application of ectomycorrhizal edible and medicinal fungi.

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