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Solid ectomycorrhizal agent and preparation method and application thereof

An ectomycorrhizal and mycorrhizal agent technology, applied in the directions of botanical equipment and methods, applications, and gardening methods, can solve problems such as affecting plant growth and plant death, and achieve a simple process, promote the growth of seedlings, and facilitate implementation. Effect

Inactive Publication Date: 2019-04-05
CHINA SHENHUA ENERGY CO LTD +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies have shown that trees with fully developed mycorrhizae are highly dependent on their symbiotic fungi. When transplanting large saplings like Pinus sylvestris sylvestris, these mycorrhizae with important functions are often destroyed, and the lack of mycorrhizal It will affect the growth of plants, and even cause the death of plants.

Method used

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  • Solid ectomycorrhizal agent and preparation method and application thereof
  • Solid ectomycorrhizal agent and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040]Embodiment 1, the preparation of solid ectomycorrhizal agent

[0041] 1. Recovery of Suillus collinitus: Take the tube containing the tested strain of Boletus collinitus out of the ultra-low temperature refrigerator, dissolve it at room temperature for 5 minutes, and transfer 10uL of the melted bacterial solution On the Pachlewski plate medium, place it in a constant temperature incubator for dark cultivation at 25°C. After mycelium grows, transfer it 2-3 times in a row, and place it in a constant temperature incubator for dark cultivation at 25°C for 7 days.

[0042] 2. Preparation of solid ectomycorrhizal agent: mix MMN liquid medium and vermiculite evenly at a volume ratio of 1:50. The humidity of the culture medium is preferably 60-70%. After mixing and bagging, sterilize at 121° C. for 20 minutes with damp heat, cool to obtain a solid culture, and set aside. In the ultra-clean workbench, inoculate the mycelium grown robustly on the Pachlewski plate obtained in step...

Embodiment 2

[0043] Embodiment 2, solid ectomycorrhizal agent inoculation experiment

[0044] 60 seedlings of Pinus sylvestris var.mongolica Litv. were reclaimed and planted at Harwusu Coal Mine Dump in Inner Mongolia Autonomous Region in March. 15cm. Carry out the following treatments respectively: wherein 30 strains adopt the solid ectomycorrhizal agent prepared by sowing method to inoculate embodiment 1 as the experimental group (+M), and the tree hole is a pit of 50cm * 50cm * 50cm (length * width * depth) (the Shuxue Pit is located in Harwusu Open-pit Coal Mine, Inner Mongolia Autonomous Region, at the open-air dump site 1km away from the mining area. The specific address is N 39°43'7.16", E111°13'7.31", altitude 1173m), The distance between the tree hole pits is 2.5m, before tree planting, evenly sprinkle the solid ectomycorrhizal agent 100g prepared by embodiment 1 at the bottom of the pit, and use the remaining 30 uninoculated Pinus sylvestris seedlings as a control group (CK ). ...

Embodiment 3

[0045] Embodiment 3, sylvestris sylvestris seedling transplanting experiment

[0046] After placing the Pinus sylvestris seedlings, cover the soil with 40cm and compact it, pour 10L of clear water into each plant to permeate the water, and cover the mulch to prevent the surface soil from drying out. In the first month, each plant was watered with 10L of water every 7 days, and in the second and third months, each plant was watered with 10L of water every half a month. After that, artificial watering was not required. The temperature and light intensity can be in accordance with the natural conditions (the daily average temperature of the site is 15°C, the daily average sunlight is 7.8 hours, and the daily average wind speed is 1.4m / s).

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Abstract

The invention discloses a solid ectomycorrhizal agent and a preparation method and application thereof. The disclosed solid ectomycorrhizal agent includes bolete hypha, vermiculite and an MMN liquid culture medium. The solid ectomycorrhizal agent improves the transplanting survival rate of pinus sylvestris nursery stocks, the growth of the nursery stocks is promoted after transplanting, and the agent plays an important role in ecological restoration of a mining area and land reclamation of the mining area.

Description

technical field [0001] The invention belongs to the fields of afforestation and soil restoration, and relates to a solid ectomycorrhizal agent and its preparation method and application. Background technique [0002] The coal mining process disturbs the natural ecosystem and destroys surface vegetation, which in turn affects the diversity of soil microorganisms and causes a series of ecological and environmental problems, such as vegetation destruction, soil erosion, and loss of biodiversity. At present, the use of artificial planting is the most common means of ecological restoration. Many areas in China use planting trees and grass to restore vegetation on the ground, especially the use of transplanting large saplings to achieve the purpose of rapid greening, such as transplanting Plant large saplings of sylvestris sylvestris, poplar, Chinese pine and other plants. However, due to the large root system of large saplings, capillary roots (functional roots, i.e. mycorrhizal...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G18/10A01G7/06A01G17/00
CPCA01G7/06A01G17/005A01G18/10
Inventor 郭洋楠贺安民潘金包玉英陈金
Owner CHINA SHENHUA ENERGY CO LTD
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