Method for detecting activity of alga hematoxin and application of method

Abstract

The invention discloses a method for detecting the activity of the alga hematoxin and application of the method. The method comprises the following steps of measuring three-dimensional fluorescence of an algae cell sample by a fluorospectro photometer, thus obtaining three-dimensional fluorescence data of the algae cell sample; converting the three-dimensional fluorescence data of the algae cell sample into a TXT file format, and eliminating rayleigh scattering of an algae three-dimensional fluorescence spectrum according to a Delaunay triangular method; performing maximum normalization on the three-dimensional fluorescence spectrum, then performing Coif2 wavelet analysis on the three-dimensional fluorescence spectrum, and selecting a fluorescence characteristic spectrum; preliminarily judging that the algae cell sample is fish toxicity red tide algae or non fish toxicity red tide algae according to the fluorescence characteristic spectrum; and performing Fisher judgment on the fluorescence characteristic spectrum so as to determine the degree of the toxicity of the algae cell sample. Through adoption of the method, the problem of time lag in the conventional detection method is solved successfully, and the method is proposed for the first in China. The problems of the conventional detection method, such as time lag and passive predicament, are solved, and the method is favorable for field detection of the fish toxicity of red tide algae.

Description

technical field

[0001] The invention relates to a detection method, in particular to a method and application for detecting the activity of algae hemolytic toxin. Background technique

[0002] In recent years, with the rapid growth of the coastal economy and the strengthening of foreign trade, the construction of ports and the continuous enhancement of ocean transportation capacity have led to a sharp increase in the eutrophication of coastal waters. Toxic and harmful red tides are on the rise, causing significant economic losses in coastal aquaculture. The study of toxic and harmful red tides has also become an important topic and content in the field of international red tide and marine environment research. On-site observation found that the red tide of Phaeocystis globosa that occurred in Zhelin Bay, Raoping, Guangdong Province in October 1997 killed all the fish cultured in the bay, with a loss of 60 million yuan; The red tide of the same species in the breeding area ...

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