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Pinus massoniana ectomycorrhizal strain coating and preparation and use methods thereof

An ectomycorrhizal and masson pine technology, applied in the field of agriculture and forestry, can solve the problems of inability to form mycorrhizal seedlings, lack of bacteria and microorganisms, complicated operation process, etc., to achieve convenient raw material acquisition, simple and easy operation steps, and high success rate of cultivation Effect

Pending Publication Date: 2022-05-17
GUIZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the application of this invention can complete the cultivation of mycorrhizal seedlings, the seedling cultivation process needs to use certain professional equipment, resulting in complicated operation process and high cost
At the same time, the invention patent No. CN110754483A discloses the application of drought-resistant water-retaining agent, adhesive, soil improver, humus and growth regulator mixed evenly until viscous, as a coating agent for pine seeds, which can improve the germination rate and Drought resistance of seedlings, but only for the development of general seedlings
Due to the lack of bacteria and microorganisms necessary for cultivating mycorrhizal seedlings in its composition, it cannot form better mycorrhizal seedlings

Method used

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  • Pinus massoniana ectomycorrhizal strain coating and preparation and use methods thereof
  • Pinus massoniana ectomycorrhizal strain coating and preparation and use methods thereof
  • Pinus massoniana ectomycorrhizal strain coating and preparation and use methods thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0038] The masson pine seed coating agent of the present embodiment, formula is:

[0039] The mycelium liquid of boletus spp., colored puffball and pine milk mushroom is mixed according to the volume ratio of 1:1:1, and the mixed mycelium liquid is 10KG, the water-retaining agent is 5Kg, the binder is 5Kg, and the humus is 20Kg. Regulator 0.005Kg, water 50Kg.

[0040] Among them, the mycelial liquid of boletus spp., colored bean puffball and pine milk mushroom is collected from the mycelium of the female species of Pinus massoniana in the state-owned Longli Forest Farm of Longli County, Qiannan Prefecture, Guizhou Province. Pack into sterilized triangular flasks with 500ML culture solution respectively, and tie the mouth of the bottle with aseptic 6 layers of gauze. In an environment of 25°C with an amplitude of 8 cm and a frequency of 120 times / min, shake culture for 7 days, and the mycelia of three kinds of mixed fungi will grow densely in the liquid.

[0041] The water re...

Embodiment 1-6

[0067] The result of embodiment 1-6 masson pine seed germination test sees figure 1 ; The results of the masson pine seed germination test of comparative example 1-5 are shown in figure 2 .

[0068] figure 1 Embodiment 1-6 ectomycorrhizal fungus coating agent masson pine seed germination statistical chart By contrasting the result of the ectomycorrhizal fungus seed coating agent masson pine seed germination experiment of embodiment 1-6 (as figure 1 shown), the germination rate, average seedling height and mycorrhizal seedling ratio obtained in Example 2 are the highest; illustrate the formula of ectomycorrhizal liquid 20L+ water retaining agent 10kg+ sticky agent 8kg+ humus 40kg+ gibberellin 0.02Kg+ purified water 40Kg In contrast, the germination of P. massoniana seeds and the proportion of mycorrhizal seedlings after coating with ectomycorrhizal fungi were the best.

[0069] figure 2 Comparative example 1-5 Statistical diagram of ectomycorrhizal fungus coated masson pi...

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Abstract

The invention discloses a pinus massoniana ectomycorrhizal strain coating agent which comprises 5-30 parts of ectomycorrhizal liquid and 5-30 parts of a water-retaining agent, 5-20 parts of an adhesive; 4-8 parts of humus soil; 20 to 50 parts of a growth regulator, and 0.005 to 0.03 part of a growth regulator; and 30-55 parts of water. The invention discloses a preparation method of a pinus massoniana ectomycorrhizal strain coating. The preparation method comprises the following steps: uniformly mixing hypha liquid containing boletus mucilaginosus, colored puffball and lactarius deliciosus, humus soil, gibberellin and an adhesive, slowly adding purified water, continuously stirring, stopping adding water when the mixture is stirred to be sticky, and standing the mixture until the mixture is in a solidified state. The use method comprises the following steps: seed disinfection: preparing 2L of 600-fold liquid from 75% chlorothalonil wettable powder with good adhesion, broad-spectrum sterilization and low toxicity, soaking seeds for 1 hour, and washing the seeds with distilled water for 3 times for later use; seed coating: coating the disinfected seeds for later use and the mixed seed coating agent in a seed coating machine, wherein the mass ratio of the seed coating agent to the seeds is 2: 1. The method has the characteristics of improving seed germination rate, promoting seedling growth and improving seedling resistance.

Description

technical field [0001] The present invention relates to the field of agriculture and forestry, in particular to the ectomycorrhizal fungus seed coating agent of Pinus massoniana, its preparation and its application method. Background technique [0002] The ectomycorrhizal fungus seed coating agent is to mix active substances such as mycelia, fertilizers, growth regulators, humus and other active substances of ectomycorrhizal fungi in proportion, and evenly wrap the seeds with inactive substances such as adhesives, and solidify Finally, a wrapping film with certain permeability is formed. The ectomycorrhizal fungus seed coating agent not only has the advantages of long fresh-keeping time, slow release of fertilizer effect, good protective effect, environmental protection and safety, but also can promote seed germination and root cluster formation, improve seedling resistance, prevent pests and diseases, etc., and finally Form mycorrhizal shoots. The research on seed coating...

Claims

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Application Information

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IPC IPC(8): A01N65/00A01N61/00A01N43/12A01P21/00A01C1/06
CPCA01N65/00A01N61/00A01N43/12A01C1/06
Inventor 郭其强丁贵杰孙学广
Owner GUIZHOU UNIV
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