8 results about "Lactarius deliciosus" patented technology

The invention discloses a method for culturing ectomycorrhiza of brilliant lactarius deliciosus, which comprises the following steps: S1, culturing brilliant lactarius deliciosus on a culture medium A to obtain a brilliant lactarius deliciosus colony; s2, disinfecting the seeds of the pinus massoniana; s3, the disinfected seeds are inoculated to a culture medium B to be cultured until the seeds germinate to form masson pine seedlings, after the masson pine seedlings grow lateral roots, the masson pine seedlings are moved to a culture medium C, the brilliant lactarius deliciosus colonies are inoculated to the edge of the roots, and ectomycorrhiza of the brilliant lactarius deliciosus on the masson pine root system is obtained after culture. Through the construction of a sterile in-vitro culture system, four defects of a traditional soil culture method are systematically solved, the research on a brilliant lactarius deliciosus-masson pine symbiosis mechanism can be accelerated, and efficient technical support is provided for mycorrhizal seedling production, forest ecological restoration and artificial domestication of edible mushrooms.

The application discloses a method for protecting the quantity of wild population of fresh Lactarius resimus in the field of mushroom conservation technology, which comprises four steps of conservation site selection, protective picking by using a mushroom protective picker to avoid damaging the mycorrhiza of the fresh Lactarius resimus during picking, seed conservation and pest control and propagation, wherein the mushroom protective picker comprises scissors, the upper surfaces of the front ends of the scissors arms of the scissors are hingedly connected with elastic sheets parallel to the scissors arms, the elastic sheets extend along the scissors arms, the ends of the two elastic sheets close to the scissors hinge are hingedly connected with a top hinge above the scissors hinge, a magnetic piece is arranged in the top hinge, an electromagnet is arranged in the scissors hinge, and the magnetic field direction of the electromagnet repels the magnetic piece. The application is simple and convenient, the elastic sheets on the scissors arms of the mushroom protective picker are used to tear the fresh Lactarius resimus from the mycorrhiza by the gap, so that the fresh-keeping time of the fresh Lactarius resimus is prolonged, the probability that the residual mycorrhiza on the ground is infected by pathogenic bacteria is reduced, and the regeneration efficiency of the wild fresh Lactarius resimus is improved.

The invention discloses an application of an ectomycorrhizal fungus inoculant in promoting growth of loblolly pine. The invention finds that ectomycorrhizal fungi lactarius deliciosus fungi can promote the growth of loblolly pine, by inoculating the lactarius deliciosus fungi on loblolly pine at a specific month age and regulating and controlling the lactarius deliciosus ectomycorrhizal fungi, the growth environment and nutritional status of the loblolly pine are improved, and the absorption of the loblolly pine on nutrients is promoted, so that the growth of the loblolly pine is effectively promoted; the mycorrhiza infection rate of the loblolly pine can be obviously improved, and the growth amount and the chlorophyll content of the loblolly pine can be obviously increased, so that a remarkable growth promoting effect on the loblolly pine is achieved.

The invention discloses an application of an ectomycorrhizal fungus inoculant in promoting the growth of slash pines. It is found that ectomycorrhizal fungi lactarius deliciosus fungi can promote growth of slash pines, the lactarius deliciosus fungi are inoculated on the slash pines at the specific month age, the growth environment and nutritional conditions of the slash pines are improved through regulation and control of the ectomycorrhizal fungi of the lactarius deliciosus, nutrient absorption of the slash pines is promoted, and then growth of the slash pines is effectively promoted; the mycorrhiza formation rate of the slash pines can be remarkably improved, and the growth amount and chlorophyll content of the slash pines are remarkably increased, so that a remarkable growth promoting effect on the slash pines is achieved.

The application relates to a Pseudomonas bacterial strain A18 and application thereof, the strain is preserved in the China Center for Type Culture Collection (CCTCC) and is classified and named as Pseudomonas glycinae A18. The application can increase pure culture Lactarius deliciosus fungal mycelium growth by adding A18 externally, promote pine-Lactarius deliciosus mycorrhizal symbiosis, increase the number of mycorrhizal tips, improve the height and biomass of mycorrhizal seedlings, and increase the nitrogen, potassium and iron absorption of the aboveground part of the mycorrhizal seedlings and the nitrogen absorption of the root system, so that the application can be applied to the synthesis and / or growth of a pine-Lactarius deliciosus symbiotic system.

The present application relates to a device for cultivating Lactarius deliciosus by using the bark of pine tree and its using method, belonging to the field of edible mushroom cultivation. The present application solves the problem that Lactarius deliciosus is rarely grown on the bark of pine tree under natural conditions due to lack of spore source, moisture environment, water source and ventilation condition. The device comprises a water bucket (1), a spore cloth (7), a water-absorbing cloth (11) and a water-repellent cloth (14). The water bucket (1) provides water source, the spore cloth (7) is inoculated with Lactarius deliciosus spores, the water-absorbing cloth (11) supplies water source and maintains humidity, and the water-repellent cloth (14) blocks wind and reduces water evaporation. The using method is as follows: preparing LBLC culture medium, i.e. 20 g of potato, 2 g of glucose, 1 g of KHPO4, 1 g of KH2PO4, 1 g of thiamine and 1000 ml of water; winding the spore cloth (7), the water-absorbing cloth (11) and the water-repellent cloth (14); and binding the water bucket (1). The present application is used for cultivating Lactarius deliciosus on the bark of pine tree.

The invention discloses a Guilin acinetobacter A59 and an application thereof. The collection number of the Guilin acinetobacter A59 is CCTCC (China Center For Type Culture Collection) NO: M 20252694. The acinetobacter Guilin A59 which is exogenously added can increase the hypha growth of pure culture lactarius deliciosus fungi, promote the symbiosis of pine-lactarius deliciosus mycorrhiza, namely increase the number of mycorrhizal tips, improve the plant height and biomass of mycorrhizal seedlings, increase the nitrogen, phosphorus, potassium and iron contents of overground parts of the mycorrhizal seedlings and the nitrogen content of root systems, and improve the growth of the pure culture lactarius deliciosus fungi. Therefore, the strain can be applied to promoting synthesis and / or growth of a pinus plant-lactarius deliciosus symbiotic system and increasing the mycorrhizal degree of mycorrhizal seedlings, so that growth and nutrient absorption of the pinus plant mycorrhizal seedlings are promoted, and the quality of ectotrophic mycorrhizal seedlings is improved.

The invention discloses a wood chip decaying device for lactarius deliciosus planting, and relates to the technical field of decaying substrate manufacturing, the wood chip decaying device comprises a decaying bin and a rotating roller which are arranged in a cylindrical shape, and the rotating roller located in the decaying bin is further sleeved with a rotating frame; the material mixing assembly is arranged at one end of the rotating frame, comprises a rotating shaft and a plurality of material turning rollers uniformly distributed on the surface of the rotating shaft, and is used for turning and mixing the stacked materials; and the material pushing assembly comprises a pushing plate slidably mounted at the other end of the rotating frame and is used for pushing the stacked materials to be discharged and cleaning the inner wall of the decaying bin. When the rotating roller drives the rotating frame to rotate, the material mixing assembly and the material pushing assembly on the rotating frame can alternately make contact with the stacked materials at the bottom of the decaying bin, and a driven gear of the material mixing assembly can be engaged and matched with an arc-shaped rack; the rotary shaft is driven to rotate, when the material turning rollers on the rotary shaft rotate, the stacked materials can be turned over, the stacked materials can be conveniently and evenly mixed, the stacked materials can be turned over regularly in the follow-up process, and aerobic respiration of bacteria in the stacked materials is promoted.