49 results about "Fungal inoculation" patented technology

The invention discloses a method for optimizing nursery-grown plant cultivation by using ectomycorrhizal fungi. The method comprises the main process including the following steps: production of an ectomycorrhizal fungi agent, cultivation of tree seedlings, inoculation with the ectomycorrhizal fungi, and nutrition management of the tree seedlings after inoculation. In actual use, a pre-experiment can be performed according to different tree species, and a best ectomycorrhizal fungi strain is selected. 6 kinds of ectomycorrhizal fungi are adopted as materials to inoculate root systems of the tree seedlings, and the growth amount and the transplanting survival rate of the tree seedlings are significantly improved.

A method for preparing forage from mulberry twigs includes such steps as pulverizing, mixing it with other raw materials, puffing, middle-or low-temp microbial fermenting, inoculating fungus, fungal fermenting, yeast fermenting, and conventional steps.

The invention relates to soil-phosphorus biological-activating fungi inoculant and a preparation method thereof; the preparation method comprises the following steps: taking a slant strain, cultivating the slant strain at 25 degrees centigrade for 1-2 days; eluting spores with 15mL of sterile water so as to prepare spore suspension, absorbing 10mL of spore suspension, inoculating the spore suspension into a conical flask containing 100mL of PD (potato dextrose) culture solution, carrying out shaking culture at 26 degrees centigrade for 3-5 days so as to obtain a liquid strain; inoculating the liquid strain in an amount of 15-20% into first-grade seed PD culture solution; carrying out cultivation at 27 degrees centigrade for 1-2 days; inoculating the first-grade seed PD culture solution in an amount of 15-20% into second-grade seed PD culture solution so as to obtain a second-grade liquid strain; and absorbing the second-liquid strain with sterile grass carbon according to a ratio of 1: 4, then, crushing so as to obtain spore powder.

The invention relates to an endophytic fungus promoting casuarina equisetifolia biomass growth. The endophytic fungus is aspergillus sp. casuarina equisetifolia rhizospheric fungus 17, which is registered and collected in China General Microbiological Culture Collection Center on June 28th, 2012. The collection number is CGMCC No. 6309. The casuarina equisetifolia rhizospheric fungus 17 is inoculated in casuarina equisetifolia hydroponic seedling. Comprehensive evaluation is carried out according to various indicators, and it is further proved that the fungus has a promotion effect upon biomass growth and an actual synergetic effect upon casuarina equisetifolia dry matters. When a functional endophytic fungus beneficial for promoting biomass growth is found, the endophytic fungus can be used in production.

A fungus (Aspergillus terreus FZC3) with the preservation number of CGMCC No.12072 can be applied to grade gentamycin, and the fungus is inoculated to a system containing gentamycin and is cultured to realize degradation of gentamycin. The fungus is cultured to remove gentamycin in environment, and can be used to process pharmaceutical wastes in order to reduce harms of gentamycin to environment, humans and animals.

The invention relates to the field of preparation of medicinal compounds, in particular to a method for efficiently preparing cyclic depsipeptide, the cyclic depsipeptide and application. The method comprises the following steps: inoculating a culture medium with the parasitic fungus Amphihorda guana LC5815 from the bat feces, and fermenting, so as to obtain a fermentation culture; and performing separating from the fermentation culture to obtain the cyclic depsipeptide compound.

Methods of production of edible filamentous fungal biomat formulations are provided as standalone protein sources and / or protein ingredients in foodstuffs as well as a one-time use or repeated use self-contained biofilm-biomat reactor comprising a container with at least one compartment and placed within the compartment(s), a feedstock, a fungal inoculum, a gas-permeable membrane, and optionally aliquid nutrient medium.

The invention provides a method for repairing heavy metal contaminated soil by combining plants and fungi, which comprises the following steps: a, immobilized culture of fungi: inoculating fungi ontodecomposed straws, and carrying out fermentation culture until the straws are overgrown with mycelia; b, fungus transplanting: transplanting the straws overgrown with the mycelia in the step a to thesurface layer of the soil, and covering the straws with a small amount of soil to enable the straws to be semi-buried in the soil; and c, planting of plants: planting plants with an enrichment effecton the heavy metals in the soil.

The invention discloses a method for improving spore yield of fungi, which comprises: inoculating fungi onto a culture medium, culturing at a proper culture temperature for 5 to 10 days, spraying 500 microliters of strong oxidizer solution at a proper concentration onto the surface of a fungus nutrition body, and blowing the fungus nutrition body with sterile wind at a speed of 0.6m / s for 10 minutes; and continuing to culture the fungi at the same culture temperature for 3 days and collecting the spores generated by the fungi. In the method, a strong oxidizer is used to arouse certain stress in the fungi to promote the fungi to grow spores which are organs more adaptive to the environment, and thus, the spore yield of the fungi is improved.

The invention relates to an endophytic fungus increasing casuarina equisetifolia chlorophyll content. The endophytic fungus is fusarium sp. casuarina equisetifolia rhizospheric fungus 46, which is registered and collected in China General Microbiological Culture Collection Center on June 28th, 2012. The collection number is CGMCC No. 6310. The casuarina equisetifolia rhizospheric fungus 46 is inoculated in casuarina equisetifolia hydroponic seedling. Comprehensive evaluation is carried out according to various indicators of chlorophyll, and it is further proved that the fungus has a promotion effect in increasing casuarina equisetifolia chlorophyll content and an actual synergetic effect upon casuarina equisetifolia chlorophyll. When a functional endophytic fungus beneficial for increasing casuarina equisetifolia chlorophyll content is found, the endophytic fungus can be used in production.

The invention provides a compound for treating and preventing diabetes and a preparation method thereof, and belongs to the technical field of microbial drugs. A preparation method of a secondary metabolite comprises the following steps: separating and purifying enteromorpha-derived fungi; inoculating fermentation liquor containing TSA and glycyrrhetinic acid with the enteromorpha-derived fungi for fermentation; and extracting, separating and purifying the secondary metabolite of enteromorpha-derived fungi. A new use of the secondary metabolite of enteromorpha-derived fungi is a new application in the preparation of alpha-glucosidase inhibitors and / or medicaments for treating diabetes. The preparation method of the secondary metabolite can improve the yield of the secondary metabolite, promote the proliferation activity of the enteromorpha-derived fungi and improve the yield of secondary metabolite. The secondary metabolite has good inhibition effect on alpha-glucosidase, slows down the generation and absorption of glucose, prevents postprandial hyperglycemia, has good effect on preventing and treating diabetes, and is easy to be absorbed by organisms.

The invention relates to a method for cultivating oyster mushrooms by utilizing pleurotus nebrodensis waste material, belongs to the technical field of cultivation of edible fungi and aims at providing a method for high-efficient cultivation of oyster mushrooms by utilizing the pleurotus nebrodensis waste material. The adopted technical scheme is as follows: the cultivation of the oyster mushrooms is performed according to the following steps: the first step: manufacturing a cultivation formula of the oyster mushrooms, firstly smashing the pleurotus nebrodensis waste material, and then preparing the cultivation formula by use of the following raw materials in percentage by weight: 67% of pleurotus nebrodensis waste material, 10% of wheat bran, 10% of corncob, 10% of fresh cotton hull, 2% of lime and 1% of CaCO3; the second step: adding water into the cultivation formula of the oyster mushrooms, which is well prepared in the first step, and fully uniformly stirring to enable the material to water ratio to be 36:64; the third step: fermenting a culture medium which is well prepared in the second step in air for 3-7 days; the fourth step: inoculating the oyster mushrooms into the culture medium after the completion of fermentation, wherein the inoculation way is open-air open type fungal inoculation. The method provided by the invention is applied to cultivation of the oyster mushrooms.

The invention relates to an endophytic fungus promoting acacia confusa biomass growth under a low-phosphorous environment. The endophytic fungus is Penicillium sp. and is registered and preserved in Center of general microbiology of China Committee for Culture Collection of Microorganisms on January 27, 2016, and the preservation number is CGMCC No.11913. Acacia confusa normal plantlets are inoculated with an acacia confusa endophytic fungus I, by measuring the heights and the biomass of plants, it is concluded that the heights and the biomass of the treated plants inoculated with the endophytic fungus I under the low-phosphorous environment are much higher than those of contrasts, it shows that the endophytic fungus I has a very large effect on promoting the plant biomass growth under the low-phosphorous environment, and therefore it is further proved that the endophytic fungus can promote the acacia confusa biomass growth under the low-phosphorous environment.

The invention relates to an endophytic fungus promoting casuarina equisetifolia photosynthesis. The endophytic fungus is aspergillussp. casuarina equisetifolia rhizospheric fungus 7, which is registered and collected in China General Microbiological Culture Collection Center on June 28th, 2012. The collection number is CGMCC No. 6305. The casuarina equisetifolia rhizospheric fungus 7 is inoculated in casuarina equisetifolia hydroponic seedling. Comprehensive evaluation is carried out according to various indicators of photosynthesis, and it is further proved that the fungus has a promotion effect upon photosynthesis. When a functional endophytic fungus beneficial for promoting photosynthesis is found, the endophytic fungus can be used in production. Through a fungus solution watering method, the endophytic fungus is inoculated on casuarina equisetifolia seedlings. The endophytic fungus has substantial effects in promoting plant photosynthesis and increasing casuarina equisetifolia dry matter accumulation.

The invention relates to an aspergillus strain capable of promoting root growth of Casuarina. The strain is Casuarina rhizosphere fungi 63 of Aspergillus sp., and is registered and preserved in China General Microbiological Culture Collection Center on June 28, 2012, with an accession No. being CGMCC No. 6311. The Casuarina rhizosphere fungi 63 is inoculated on hydroponic seedlings of the Casuarina, and a comprehensively evaluation is carried out according to various indicators of root growth, and promotion effects of the fungi on root growth is further confirmed, thereby finding a functional endophytic fungi which is benefit to promote root growth and can be applied in production.

The invention relates to the technical field of soil repairing and discloses a method for adsorbing heavy metals in soil through plant-microorganism combination. The method is a plant-microorganism combined repairing technique with artemisia sacrorum as a plant material and VA (vesicular-arbuscular) fungi as a microorganism material. Soil of a part at a depth of 30cm under artemisia sacrorum is collected and screened to collect exposed VA fungal spores. A mixture of river mud and clay is added to a beef extract-peptone medium, and sterilization and disinfection are implemented. The obtained VAfungal spores are inoculated into the medium for culture. The artemisia sacrorum seeds are sterilized with a hydrogen peroxide solution and then soaked in warm water. The obtained VA fungi are inoculated into soil containing heavy metals, and are inoculated in multiple layers to ensure the inoculation amount of the spores, and the treated artemisia sacrorum seeds are put into the soil. As the artemisia sacrorum grows, the VA fungi can be formed. Compared with a conventional plant-microorganism combined repairing technique, the method has a good Cu adsorption effect which is up to 85%.

The invention discloses a method for inoculating fungi, an inoculation appliance and a culture medium. The inoculation appliance and the culture medium are applied to the method. The method for inoculating the fungi includes sterilizing the metal inoculation appliance; transferring the inoculation appliance to the culture medium with the target fungi in a sterile environment and then cultivating the culture medium; contaminating inoculums formed by wood or bamboo components on the inoculation appliance with the fungi; taking out the inoculation appliance and piecing an inoculation position of a host by the aid of a tip of the inoculation appliance; infecting the host with the fungi on the contaminated wood or bamboo inoculums on the inoculation appliance to finally implement inoculation operation. The tip is arranged at an end of the metal inoculation appliance, and the wood or bamboo components are arranged on the inoculation appliance. The method, the inoculation appliance and the culture medium have the advantages of accuracy, convenience, capability of saving time and labor and the like. Besides, the fungi can be inoculated on hard portions of woods and the like by the aid of the method, the inoculation appliance and the culture medium.

The present invention relates to an Aspergillus strain capable of promoting the growth of casuarina root system, which is Aspergillus sp. Casuarina rhizosphere fungus 63, which has been registered and preserved in the General Microorganism Center of China Microbiological Culture Collection Management Committee on June 28, 2012. The deposit number is CGMCCNo.6311. Casuarina rhizosphere fungus 63 was inoculated into casuarina hydroponic seedlings, and according to the comprehensive evaluation of various indicators of root growth, it was further confirmed that it can promote root growth. Finding functional endophytic fungi that are beneficial to root growth can be applied to Production.

The invention discloses a mycorrhizal light-medium container seedling raising method for tectorta grandis. The mycorrhizal light-medium container seedling raising method specifically includes the steps of expanding propagation of inoculation bacterial agents, seed collection, germination, light-medium cup preparation, cup inoculation, and seedling cultivation, management and protection. After seeds of the tectorta grandis are germinated, obtained buds and seedlings are planted in non-woven three-dimensional cups filled with light media, fungal inoculation is carried out to realize seedling mycorrhization, and high-quality container seedlings of the tectorta grandis are obtained through seedling cultivation, management and protection. The mycorrhizal light-medium container seedling raisingmethod has the advantages that breeding and cultivating of the seeds of the tectorta grandis are enhanced, the ability of root systems of the tectorta grandis to absorb moisture and mineral substancesis improved by mycorrhizas, and the drought resistance and afforestation survival rate of plants are increased.

The invention discloses a method for constructing coexistence of aerobic fungi and facultative or anaerobic microorganisms by utilizing 3D printing, which comprises the following steps: inoculating the aerobic fungi into a fermentation culture medium, and placing a supporting material with pores on the fermentation culture medium, so that the aerobic fungi form a compact biological membrane on the supporting material; facultative or anaerobic microorganisms are made into a living body material of a fence structure through 3D printing; and putting the living body material into a fermentation culture medium, coexisting with aerobic fungi, and carrying out fermentation reaction. The invention also provides a bioreactor constructed by using the 3D printed container and the living body material. According to the invention, oxygen consumption is realized through biological membranes formed by aerobic fungi on a support material and a living body material, proper growth and production conditions are created for facultative or anaerobic microorganisms in the living body material, and a bioreactor is designed through the living body material and 3D printing equipment; the oxygen gradient is generated in the bioreactor by utilizing the oxygen consumption of aerobic bacteria, and the oxygen requirement of chemical production based on a CBP system is met.

Methods of producing edible filamentous fungal biomat formulations as separate protein sources and / or protein components in food products and disposable or reusable self-contained biomat reactors are provided, the reactor includes a container having at least one compartment and a feedstock, a fungal inoculum, a gas permeable membrane, and optionally a liquid nutrient medium disposed within the compartment.

The invention discloses a corn naringenin methyltransferase gene ZmNOMT. The cDNA (complementary deoxyribonucleic acid) nucleotide sequence of the corn naringenin methyltransferase gene ZmNOMT is shown as SEQ ID No.1; the invention also discloses an application of improving the broad-spectrum disease resistance of plants and an application of improving the naringenin content by reducing the expression of the corn naringenin methyltransferase gene ZmNOMT. Experiments prove that through inoculation identification of pathogenic fungi of a ZmNOMT transgenic overexpression positive strain, it is found that the ZmNOMT overexpression positive strain shows a susceptible phenotype to various corn diseases, and it is proved that ZmNOMT is a new susceptible gene. Two target spots are designed for the ZmNOMT by further utilizing a CRISPR / CAS9 technology, gene editing is carried out on the ZmNOMT, and it is found that the naringenin content in a ZmNOMT editing strain is remarkably improved; the ZmNOMT edited strain is subjected to pathogenic bacterium inoculation identification, and the ZmNOMT edited strain shows broad-spectrum disease resistance to various corn fungal diseases. The invention provides a new gene, a new material and a new method for disease-resistant breeding of corn, and the application prospect is wide.

The invention discloses a teak mycorrhized light substrate container seedling raising method, the specific steps of which include inoculation bacteria agent propagation and cultivation, seed collection, germination, light substrate cup preparation, cup inoculation, nursery stock cultivation and management and protection, and teak seeds After accelerated germination, the obtained sprouts are planted in non-woven three-dimensional cups equipped with light substrates, and fungal inoculation is carried out at the same time to achieve mycorrhization of seedlings, and then high-quality teak container seedlings are obtained through seedling cultivation and management. The method is beneficial to strengthening the propagation and cultivation of the teak tree species, and the mycorrhizae are beneficial to enhancing the water and mineral absorption capacity of the teak root system, and improving the drought resistance of the plant and the survival rate of afforestation.

The present invention relates to fungi of Acremonium spp, wherein said fungi are purified or isolated from plants of the Brachiaria-Urochloa complex and wherein, when said fungi are inoculated into a plant, said plant has improved resistance to diseases and / or pests relative to an uninocualated control plant. The present invention also relates to plants inoculated with such fungi, products produced by the fungi and related genes, proteins and methods.

The invention discloses a method for treating sludge hydrolysate by fungi and recovering mycothalli. The method comprises the following steps: (1) preparing the sludge hydrolysate: carrying out pyrohydrolysis on sludge, carrying out solid-liquid separation, and taking a liquid part to obtain the sludge hydrolysate; (2) treatment of the sludge hydrolysate: sterilizing the sludge hydrolysate; (3) inoculation with fungi: inoculating the fungi into the solution sterilized in the step (2), and culturing; and (4) recycling of the mycothalli: after the culture is finished, separating the mycothalli and recycling the mycothalli. High-concentration organic matters in the sludge hydrolysate are removed by the decomposition effect of the fungi on the organic matters; meanwhile, the fungus strain takes the sludge hydrolysate as a culture medium, and mycelia are formed by growth and enrichment of the organic matters in the sludge hydrolysate, so that obtaining of the high-purity mycothalli and recycling of the subsequent mycelia can be facilitated, a recycling effect is achieved, and the method has a wide application prospect in the field of sludge treatment.

The invention relates to an endophytic fungus promoting casuarina equisetifolia photosynthesis. The endophytic fungus is aspergillussp. casuarina equisetifolia rhizospheric fungus 7, which is registered and collected in China General Microbiological Culture Collection Center on June 28th, 2012. The collection number is CGMCC No. 6305. The casuarina equisetifolia rhizospheric fungus 7 is inoculated in casuarina equisetifolia hydroponic seedling. Comprehensive evaluation is carried out according to various indicators of photosynthesis, and it is further proved that the fungus has a promotion effect upon photosynthesis. When a functional endophytic fungus beneficial for promoting photosynthesis is found, the endophytic fungus can be used in production. Through a fungus solution watering method, the endophytic fungus is inoculated on casuarina equisetifolia seedlings. The endophytic fungus has substantial effects in promoting plant photosynthesis and increasing casuarina equisetifolia dry matter accumulation.

The invention discloses a traditional Chinese medicine isatis root residue water treatment agent, and a preparation method and application thereof, and belongs to the technical field of water treatment agents. The problem that existing wastewater and traditional Chinese medicine residues urgently need to be treated is effectively solved. The traditional Chinese medicine isatis root residue water treatment agent is prepared by sequentially carrying out NaOH modification and white-rot fungus fermentation treatment on isatis roots which are decocted with water. The method comprises the followingspecific steps: firstly, decolorizing the traditional Chinese medicine isatis root residues; then adding NaOH into the decolorized isatis root residues to carry out chemical modification; mixing the chemically modified isatis root residues with carbon source and water, cooling, sterilizing, adding white-rot fungi to inoculate, and fermenting; and finally, filtering out a solid from a fermentationproduct, and drying the solid to obtain the water treatment agent. The water treatment agent has the following beneficial effects: the isatis root residues are used as a raw material to prepare a biological adsorbent through a chemical and biological double-treatment method, and the isatis root residues which are Chinese herbal medicine wastes are used for treating wastewater, so that the utilization rate of resources can be effectively improved, and the environmental pollution is reduced.

The invention relates to a phyllosticta strain promoting casuarina equisetifolia photosynthesis. The strain is phyllosticta sp. casuarina equisetifolia rhizospheric fungus 16, which is registered and collected in China General Microbiological Culture Collection Center on June 28th, 2012. The collection number is CGMCC No. 6308. The casuarina equisetifolia rhizospheric fungus 16 is inoculated in casuarina equisetifolia hydroponic seedling. Comprehensive evaluation is carried out according to various indicators of photosynthesis, and it is further proved that the strain has a promotion effect upon photosynthesis and an actual synergetic effect upon casuarina equisetifolia dry matters. When a functional endophytic fungus beneficial for promoting photosynthesis is found, the strain can be used in production.