47 results about "Fungal inoculation" patented technology

The invention discloses a method for optimizing nursery-grown plant cultivation by using ectomycorrhizal fungi. The method comprises the main process including the following steps: production of an ectomycorrhizal fungi agent, cultivation of tree seedlings, inoculation with the ectomycorrhizal fungi, and nutrition management of the tree seedlings after inoculation. In actual use, a pre-experiment can be performed according to different tree species, and a best ectomycorrhizal fungi strain is selected. 6 kinds of ectomycorrhizal fungi are adopted as materials to inoculate root systems of the tree seedlings, and the growth amount and the transplanting survival rate of the tree seedlings are significantly improved.

The invention relates to soil-phosphorus biological-activating fungi inoculant and a preparation method thereof; the preparation method comprises the following steps: taking a slant strain, cultivating the slant strain at 25 degrees centigrade for 1-2 days; eluting spores with 15mL of sterile water so as to prepare spore suspension, absorbing 10mL of spore suspension, inoculating the spore suspension into a conical flask containing 100mL of PD (potato dextrose) culture solution, carrying out shaking culture at 26 degrees centigrade for 3-5 days so as to obtain a liquid strain; inoculating the liquid strain in an amount of 15-20% into first-grade seed PD culture solution; carrying out cultivation at 27 degrees centigrade for 1-2 days; inoculating the first-grade seed PD culture solution in an amount of 15-20% into second-grade seed PD culture solution so as to obtain a second-grade liquid strain; and absorbing the second-liquid strain with sterile grass carbon according to a ratio of 1: 4, then, crushing so as to obtain spore powder.

The invention relates to an endophytic fungus promoting casuarina equisetifolia biomass growth. The endophytic fungus is aspergillus sp. casuarina equisetifolia rhizospheric fungus 17, which is registered and collected in China General Microbiological Culture Collection Center on June 28th, 2012. The collection number is CGMCC No. 6309. The casuarina equisetifolia rhizospheric fungus 17 is inoculated in casuarina equisetifolia hydroponic seedling. Comprehensive evaluation is carried out according to various indicators, and it is further proved that the fungus has a promotion effect upon biomass growth and an actual synergetic effect upon casuarina equisetifolia dry matters. When a functional endophytic fungus beneficial for promoting biomass growth is found, the endophytic fungus can be used in production.

The invention relates to the field of preparation of medicinal compounds, in particular to a method for efficiently preparing cyclic depsipeptide, the cyclic depsipeptide and application. The method comprises the following steps: inoculating a culture medium with the parasitic fungus Amphihorda guana LC5815 from the bat feces, and fermenting, so as to obtain a fermentation culture; and performing separating from the fermentation culture to obtain the cyclic depsipeptide compound.

Methods of production of edible filamentous fungal biomat formulations are provided as standalone protein sources and/or protein ingredients in foodstuffs as well as a one-time use or repeated use self-contained biofilm-biomat reactor comprising a container with at least one compartment and placed within the compartment(s), a feedstock, a fungal inoculum, a gas-permeable membrane, and optionally aliquid nutrient medium.

The invention provides a method for repairing heavy metal contaminated soil by combining plants and fungi, which comprises the following steps: a, immobilized culture of fungi: inoculating fungi ontodecomposed straws, and carrying out fermentation culture until the straws are overgrown with mycelia; b, fungus transplanting: transplanting the straws overgrown with the mycelia in the step a to thesurface layer of the soil, and covering the straws with a small amount of soil to enable the straws to be semi-buried in the soil; and c, planting of plants: planting plants with an enrichment effecton the heavy metals in the soil.

The invention discloses a method for improving spore yield of fungi, which comprises: inoculating fungi onto a culture medium, culturing at a proper culture temperature for 5 to 10 days, spraying 500 microliters of strong oxidizer solution at a proper concentration onto the surface of a fungus nutrition body, and blowing the fungus nutrition body with sterile wind at a speed of 0.6m/s for 10 minutes; and continuing to culture the fungi at the same culture temperature for 3 days and collecting the spores generated by the fungi. In the method, a strong oxidizer is used to arouse certain stress in the fungi to promote the fungi to grow spores which are organs more adaptive to the environment, and thus, the spore yield of the fungi is improved.

The invention provides a compound for treating and preventing diabetes and a preparation method thereof, and belongs to the technical field of microbial drugs. A preparation method of a secondary metabolite comprises the following steps: separating and purifying enteromorpha-derived fungi; inoculating fermentation liquor containing TSA and glycyrrhetinic acid with the enteromorpha-derived fungi for fermentation; and extracting, separating and purifying the secondary metabolite of enteromorpha-derived fungi. A new use of the secondary metabolite of enteromorpha-derived fungi is a new application in the preparation of alpha-glucosidase inhibitors and/or medicaments for treating diabetes. The preparation method of the secondary metabolite can improve the yield of the secondary metabolite, promote the proliferation activity of the enteromorpha-derived fungi and improve the yield of secondary metabolite. The secondary metabolite has good inhibition effect on alpha-glucosidase, slows down the generation and absorption of glucose, prevents postprandial hyperglycemia, has good effect on preventing and treating diabetes, and is easy to be absorbed by organisms.

The invention relates to an endophytic fungus promoting acacia confusa biomass growth under a low-phosphorous environment. The endophytic fungus is Penicillium sp. and is registered and preserved in Center of general microbiology of China Committee for Culture Collection of Microorganisms on January 27, 2016, and the preservation number is CGMCC No.11913. Acacia confusa normal plantlets are inoculated with an acacia confusa endophytic fungus I, by measuring the heights and the biomass of plants, it is concluded that the heights and the biomass of the treated plants inoculated with the endophytic fungus I under the low-phosphorous environment are much higher than those of contrasts, it shows that the endophytic fungus I has a very large effect on promoting the plant biomass growth under the low-phosphorous environment, and therefore it is further proved that the endophytic fungus can promote the acacia confusa biomass growth under the low-phosphorous environment.

The invention relates to an endophytic fungus promoting casuarina equisetifolia photosynthesis. The endophytic fungus is aspergillussp. casuarina equisetifolia rhizospheric fungus 7, which is registered and collected in China General Microbiological Culture Collection Center on June 28th, 2012. The collection number is CGMCC No. 6305. The casuarina equisetifolia rhizospheric fungus 7 is inoculated in casuarina equisetifolia hydroponic seedling. Comprehensive evaluation is carried out according to various indicators of photosynthesis, and it is further proved that the fungus has a promotion effect upon photosynthesis. When a functional endophytic fungus beneficial for promoting photosynthesis is found, the endophytic fungus can be used in production. Through a fungus solution watering method, the endophytic fungus is inoculated on casuarina equisetifolia seedlings. The endophytic fungus has substantial effects in promoting plant photosynthesis and increasing casuarina equisetifolia dry matter accumulation.

The invention relates to the technical field of soil repairing and discloses a method for adsorbing heavy metals in soil through plant-microorganism combination. The method is a plant-microorganism combined repairing technique with artemisia sacrorum as a plant material and VA (vesicular-arbuscular) fungi as a microorganism material. Soil of a part at a depth of 30cm under artemisia sacrorum is collected and screened to collect exposed VA fungal spores. A mixture of river mud and clay is added to a beef extract-peptone medium, and sterilization and disinfection are implemented. The obtained VAfungal spores are inoculated into the medium for culture. The artemisia sacrorum seeds are sterilized with a hydrogen peroxide solution and then soaked in warm water. The obtained VA fungi are inoculated into soil containing heavy metals, and are inoculated in multiple layers to ensure the inoculation amount of the spores, and the treated artemisia sacrorum seeds are put into the soil. As the artemisia sacrorum grows, the VA fungi can be formed. Compared with a conventional plant-microorganism combined repairing technique, the method has a good Cu adsorption effect which is up to 85%.

The invention discloses a mycorrhizal light-medium container seedling raising method for tectorta grandis. The mycorrhizal light-medium container seedling raising method specifically includes the steps of expanding propagation of inoculation bacterial agents, seed collection, germination, light-medium cup preparation, cup inoculation, and seedling cultivation, management and protection. After seeds of the tectorta grandis are germinated, obtained buds and seedlings are planted in non-woven three-dimensional cups filled with light media, fungal inoculation is carried out to realize seedling mycorrhization, and high-quality container seedlings of the tectorta grandis are obtained through seedling cultivation, management and protection. The mycorrhizal light-medium container seedling raisingmethod has the advantages that breeding and cultivating of the seeds of the tectorta grandis are enhanced, the ability of root systems of the tectorta grandis to absorb moisture and mineral substancesis improved by mycorrhizas, and the drought resistance and afforestation survival rate of plants are increased.

The present invention relates to fungi of Acremonium spp, wherein said fungi are purified or isolated from plants of the Brachiaria-Urochloa complex and wherein, when said fungi are inoculated into a plant, said plant has improved resistance to diseases and/or pests relative to an uninocualated control plant. The present invention also relates to plants inoculated with such fungi, products produced by the fungi and related genes, proteins and methods.

The invention discloses a method for treating sludge hydrolysate by fungi and recovering mycothalli. The method comprises the following steps: (1) preparing the sludge hydrolysate: carrying out pyrohydrolysis on sludge, carrying out solid-liquid separation, and taking a liquid part to obtain the sludge hydrolysate; (2) treatment of the sludge hydrolysate: sterilizing the sludge hydrolysate; (3) inoculation with fungi: inoculating the fungi into the solution sterilized in the step (2), and culturing; and (4) recycling of the mycothalli: after the culture is finished, separating the mycothalli and recycling the mycothalli. High-concentration organic matters in the sludge hydrolysate are removed by the decomposition effect of the fungi on the organic matters; meanwhile, the fungus strain takes the sludge hydrolysate as a culture medium, and mycelia are formed by growth and enrichment of the organic matters in the sludge hydrolysate, so that obtaining of the high-purity mycothalli and recycling of the subsequent mycelia can be facilitated, a recycling effect is achieved, and the method has a wide application prospect in the field of sludge treatment.

The invention discloses a traditional Chinese medicine isatis root residue water treatment agent, and a preparation method and application thereof, and belongs to the technical field of water treatment agents. The problem that existing wastewater and traditional Chinese medicine residues urgently need to be treated is effectively solved. The traditional Chinese medicine isatis root residue water treatment agent is prepared by sequentially carrying out NaOH modification and white-rot fungus fermentation treatment on isatis roots which are decocted with water. The method comprises the followingspecific steps: firstly, decolorizing the traditional Chinese medicine isatis root residues; then adding NaOH into the decolorized isatis root residues to carry out chemical modification; mixing the chemically modified isatis root residues with carbon source and water, cooling, sterilizing, adding white-rot fungi to inoculate, and fermenting; and finally, filtering out a solid from a fermentationproduct, and drying the solid to obtain the water treatment agent. The water treatment agent has the following beneficial effects: the isatis root residues are used as a raw material to prepare a biological adsorbent through a chemical and biological double-treatment method, and the isatis root residues which are Chinese herbal medicine wastes are used for treating wastewater, so that the utilization rate of resources can be effectively improved, and the environmental pollution is reduced.