An endophytic fungus that promotes the uptake of nutrients in Acacia taiwanensis under low phosphorus environment
A technology of nutrient elements and endophytic fungi, applied in the direction of fungi, biocides, microorganisms, etc.
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[0021] 1. Application of rhizosphere soil watering and seedling direct inoculation
[0022] This experiment adopts soil culture pot experiment, and the material used in the experiment is Taiwan Acacia annual seedling provided by Forestry Bureau of Zhangzhou City, Fujian Province. Select Taiwan acacia seedlings with consistent growth to plant in diameter 15cm, high 10cm plastic pots. The soil used is yellow heart soil that has been strictly fumigated. After mixing and weighing, put an equal amount of 3kg of soil into each pot. After one month of restorative growth, the rhizosphere of Acacia Taiwan was applied at a concentration of 5.5×10 6 100mL bacterial solution of cfu / ml, use distilled water as blank control.
[0023] Bacterial liquid preparation: Inject the test strain into 40mL liquid medium, the medium is modified Martin's medium, in which peptone 5.0g / L, yeast extract powder 2.0g / L, glucose 20.0g / L, dipotassium hydrogen phosphate 1.0 g / L, magnesium sulfate 0.5g / L, an...
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