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Method for inoculating Fungi

A fungal, cutting-edge technology, applied in botany equipment and methods, horticulture, plant cultivation, etc., can solve the problems of complicated preparation methods, achieve the effects of tissue stabbing, precise fixed-point observation and statistics, and time saving

Inactive Publication Date: 2016-08-24
LANZHOU UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But this kind of wooden stick strain is not suitable for fungal inoculation, and its preparation method is too complicated

Method used

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  • Method for inoculating Fungi
  • Method for inoculating Fungi
  • Method for inoculating Fungi

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0034] The following are examples of the present invention.

[0035] An example of the inoculation tool of the present invention is figure 1 As shown, it is composed of a rod-shaped metal piece 1 and a wooden or bamboo inoculum 2 embedded in the rod-shaped metal piece. The front end of the rod-shaped metal piece 1 is a tip portion. In actual preparation, it can be as attachedfigure 1 Offer a through hole on the bar-shaped metal part 1 as shown, then inlay a piece of inoculum 2 by wood or bamboo material in the through hole, the two ends of inoculum 2 will be exposed to contact with cultured bacteria like this. The inoculation tool can also be made to provide a blind hole on the rod-shaped metal part 1, and inlay a piece of inoculum by wood or bamboo material in the blind hole. In this structure, the inoculum will only have one exposed outside. The top of the hole can be provided with a top wire, and the rotation of the top wire can push the inoculum inlaid into the blind hole...

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Abstract

The invention discloses a method for inoculating fungi, an inoculation appliance and a culture medium. The inoculation appliance and the culture medium are applied to the method. The method for inoculating the fungi includes sterilizing the metal inoculation appliance; transferring the inoculation appliance to the culture medium with the target fungi in a sterile environment and then cultivating the culture medium; contaminating inoculums formed by wood or bamboo components on the inoculation appliance with the fungi; taking out the inoculation appliance and piecing an inoculation position of a host by the aid of a tip of the inoculation appliance; infecting the host with the fungi on the contaminated wood or bamboo inoculums on the inoculation appliance to finally implement inoculation operation. The tip is arranged at an end of the metal inoculation appliance, and the wood or bamboo components are arranged on the inoculation appliance. The method, the inoculation appliance and the culture medium have the advantages of accuracy, convenience, capability of saving time and labor and the like. Besides, the fungi can be inoculated on hard portions of woods and the like by the aid of the method, the inoculation appliance and the culture medium.

Description

technical field [0001] The present invention relates to a fungal inoculation method, and the inoculation utensil and culture medium used in the method. Specifically, it is an inoculation method in which the target fungus is contacted with the inoculated part of the host plant by using the inoculation tool, and the fungus invades the host plant. Inoculation equipment and culture medium. Background technique [0002] The process of artificially contacting pathogenic fungi with the susceptible parts of the host plant to create conditions for the pathogen to invade and induce the disease of the host is called inoculation. Inoculation is an important step in the process of syndrome and disease. Inoculation is required when studying the pathogenesis of parasitic phenomena, measuring the disease resistance of varieties, and evaluating the effect of pesticides on disease prevention. Therefore, the inoculation method directly affects the test effect. [0003] The method of artifici...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04
CPCA01G18/00
Inventor 俞斌华李彦忠南志标韩海斌徐林波
Owner LANZHOU UNIVERSITY
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