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Method for adsorbing heavy metals in soil through plant-microorganism combination

A heavy metal and microbial technology, applied in the field of soil remediation, can solve the problems of low cost, groundwater infestation, and obvious treatment effect, and achieve the effects of simple preparation process, beautifying the environment, and low price

Inactive Publication Date: 2020-02-07
LIAONING UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] At present, soil problems are gradually revealing hazards, especially the problem of excessive heavy metal content in soil is becoming more and more serious, and excessive heavy metals in soil will cause many hazards
Because heavy metals cannot be degraded by microorganisms, heavy metals will remain in the soil for a long time and are difficult to handle. Long-term retention in the soil will affect the development of plant roots and leaves, infect groundwater, and affect human survival and health.
However, the current treatment technology for heavy metals in soil is time-consuming, labor-intensive and expensive, and the method of using arbuscular fungi (VA fungi) to cooperate with plants to adsorb heavy metals in soil is low in cost and has obvious treatment effects on copper and lead.

Method used

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  • Method for adsorbing heavy metals in soil through plant-microorganism combination
  • Method for adsorbing heavy metals in soil through plant-microorganism combination
  • Method for adsorbing heavy metals in soil through plant-microorganism combination

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Effect test

Embodiment 1

[0023] Embodiment 1 The method of plant-microbe joint adsorption of heavy metals in soil

[0024] 1. Collection of VA fungal spores:

[0025] 1) Select the tall and healthy Artemisia japonica tree for excavation, and collect 50g of soil at a depth of 30cm from the ground.

[0026] 2) Sieve the collected soil with a 100-mesh sieve.

[0027] 3) Collect the exposed VA fungal spores.

[0028] 2. Preparation of culture medium

[0029] 1) Preparation of beef extract-peptone medium: 3 g of beef extract; 10 g of peptone; 5 g of sodium chloride; 15 g of agar;

[0030] 2) Add 10 g of river mud and soil to 50 g of beef extract peptone medium.

[0031] 3) Put the medium obtained in step 2) into a high-temperature steam sterilizer at 121° C. for 15-20 minutes to sterilize. Then use 15 g of 20% hydrogen peroxide solution for fumigation for 15 minutes.

[0032] 3. Cultivation of VA fungi:

[0033] The obtained arbuscular fungus spores were inoculated on the culture medium by plate coa...

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Abstract

The invention relates to the technical field of soil repairing and discloses a method for adsorbing heavy metals in soil through plant-microorganism combination. The method is a plant-microorganism combined repairing technique with artemisia sacrorum as a plant material and VA (vesicular-arbuscular) fungi as a microorganism material. Soil of a part at a depth of 30cm under artemisia sacrorum is collected and screened to collect exposed VA fungal spores. A mixture of river mud and clay is added to a beef extract-peptone medium, and sterilization and disinfection are implemented. The obtained VAfungal spores are inoculated into the medium for culture. The artemisia sacrorum seeds are sterilized with a hydrogen peroxide solution and then soaked in warm water. The obtained VA fungi are inoculated into soil containing heavy metals, and are inoculated in multiple layers to ensure the inoculation amount of the spores, and the treated artemisia sacrorum seeds are put into the soil. As the artemisia sacrorum grows, the VA fungi can be formed. Compared with a conventional plant-microorganism combined repairing technique, the method has a good Cu adsorption effect which is up to 85%.

Description

technical field [0001] The invention relates to the technical field of soil remediation, in particular to a plant-microorganism joint adsorption method for heavy metals in soil. Background technique [0002] At present, soil problems are gradually showing hazards, especially the problem of excessive heavy metal content in soil is becoming more and more serious, and excessive heavy metals in soil will cause many hazards. Since heavy metals cannot be degraded by microorganisms, heavy metals will remain in the soil for a long time and are difficult to handle. Long-term retention in the soil will affect the development of plant roots and leaves, infect groundwater, and affect human survival and health. However, the current treatment technology for heavy metals in soil is time-consuming, labor-intensive and expensive, while the method of using arbuscular fungi (VA fungi) in combination with plants to adsorb heavy metals in soil is low in cost and has obvious treatment effects on ...

Claims

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Application Information

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IPC IPC(8): B09C1/00B09C1/10
CPCB09C1/105
Inventor 包红旭刘斌李良玉吕志忠宋泽斌徐晓红
Owner LIAONING UNIVERSITY
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