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Edible composition with filamentous fungi and bioreactor system for the cultivation thereof

A filamentous fungus, biological technology, applied in special-purpose bioreactors/fermenters, microbial-based methods, microorganisms, etc., can solve the problem of inaccessible or limited access to sterile facilities or/or need to be sealed aseptic System and other issues

Pending Publication Date: 2020-03-24
SUSTAINABLE BIOPRODUCTS INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Often these types of environments have no or limited access to aseptic facilities or / or require sealed aseptic systems to fully contain waste streams and / or food, fuel and materials produced

Method used

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  • Edible composition with filamentous fungi and bioreactor system for the cultivation thereof
  • Edible composition with filamentous fungi and bioreactor system for the cultivation thereof
  • Edible composition with filamentous fungi and bioreactor system for the cultivation thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0150] Example 1: Growth of the strain Fusarium oxysporum strain MK7 and other fungi in static tray reactors.

[0151] Filamentous acidophilus Fusarium oxysporum strain MK 7, Ganoderma lucidum ( figure 1 A) Pleurotus officinale (Pearl oyster mushroom, figure 1 B: and blue oyster mushroom, figure 1 C), hydrangea (broccoli; figure 1 D), elm mushroom ( figure 1 E); big bald puffball ( figure 1 F) and Fusarium venenatum biomat, as described in PCT / US2017 / 020050.

Embodiment 2

[0152] Example 2. Growth of Fusarium oxysporum MK7 biomats on nutrient medium changed daily (semi-static conditions).

[0153] Compact biomats of Fusarium oxysporum strain MK7 were grown approximately 3 cm thick in nutrient medium changed daily for 21 days. Used in 12.7x 17.8cm Sterile MK7-1 broth (described in PCT / US2017 / 020050) containing 7.5% glycerol at pH 3.0 in glass trays generated biomats. To start the experiment, 200 mL of nutrient medium was inoculated with a 5% (v / v) culture of Fusarium oxysporum strain MK7 during the late exponential growth phase as previously described in PCT / US2017 / 020050. 200 mL of inoculated medium was added to each of three sterile trays lined with sterile coarse nylon mesh screens. The culture was incubated undisturbed at room temperature (approximately 22°C) for 4 days to form an initial biocushion that formed at the liquid surface. After 4 days of growth, gently lift the biomat out of the tray using a nylon mesh screen and tilt it at a ...

Embodiment 3

[0154] Example 3. Growth of biomats under continuous flow conditions.

[0155] Fabricate a continuous flow bioreactor system to demonstrate the growth of biomats on the surface of flowing liquid media. The system is made of a 2.44 m long transparent plastic cover sheet with a series of corrugations used as flow channels ( image 3 ). The ends of each channel were capped with silicon (100% silicone, DAP Products Inc., Baltimore, MD) to allow liquid to remain within the channel. Flow through the channel is facilitated by delivering liquid medium via a peristaltic pump to one end of the channel, and the liquid exits the other end of the channel through a hole in the bottom of the channel. The entire plastic cover plate system is sloped at an angle of 1 cm rise per 1 m run so that about 500 mL of liquid can be retained in each channel and the constant flow is a function of liquid volume and slope angle.

[0156] Sterilize the plate system and use A sample plastic wrap is used...

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Abstract

Methods of production of edible filamentous fungal biomat formulations are provided as standalone protein sources and / or protein ingredients in foodstuffs as well as a one-time use or repeated use self-contained biofilm-biomat reactor comprising a container with at least one compartment and placed within the compartment(s), a feedstock, a fungal inoculum, a gas-permeable membrane, and optionally aliquid nutrient medium.

Description

[0001] field of invention [0002] The present application relates to edible fungi and provides methods for the preparation of edible fungi for food, liquid and solid formulations of edible fungi and uses and methods related thereto, foodstuffs containing edible fungi and methods and uses thereof. [0003] Background of the invention [0004] The United Nations put the world population at 7.5 billion in August 2017 and predicted that number would grow to 8 billion in 2023 and 10 billion in 2056. In a related report, the Food and Agriculture Organization of the United Nations (FAO) estimated that if the global population reaches 9.1 billion by 2050, world food production will need to increase by 70%, and double in developing countries. Food production will need to increase despite rising energy costs, dwindling aquifer resources, loss of farmland to urban sprawl, and increasingly severe weather caused by climate change (e.g. higher temperatures, increased droughts, flooding, etc...

Claims

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Application Information

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IPC IPC(8): A23L33/195C12R1/77A23J3/20C12R1/645A23L31/00A01G18/64A01G18/66
CPCA23C9/1203A23J3/20A23L33/195A23L31/00A23L29/065C12N1/14C12R2001/77C12N1/145A23J3/227A23V2250/208C12M23/40C12M21/00A23G9/36C12M23/24A23V2002/00C12M23/34A23G3/366A23V2200/13A23V2300/38
Inventor M.A.科祖贝尔R.E.麦柯Y.C.阿维尼尔M.D.汉密尔顿
Owner SUSTAINABLE BIOPRODUCTS INC
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